The invention discloses a method for manufacturing medical nanometer fiber protective clothing fabric based on the electrospinning technology. According to the method, spunbond, or traditional fabricor other textile fiber materials is used as base fabric, by utilizing the electrospinning technology, in combination with a doping and modifying technology and a long-acting electret technology, nanometer fiber membranes with different functionality are sprayed to the surface of the base fabric, and then the medical nanometer fiber protective clothing fabric integrating a protective function, a comfort function, an anti-microbial function, an anti-static function, a heat conducting function and the like and combining thermal-wet comfort and protective safety is manufactured and obtained. The method mainly solves the problem that an existing protective clothing fabric is large in filtration resistance, and poor in air permeability and comfort, and the method has the technological features that the manufacturing technology is simple, cost is low, and functional finishing combination is easy. Compared with a traditional process of manufacturing the protective clothing fabric, such as a compact fabric technology, a coating technology and a composite microporous membrane technology, the method omits the procedures of spinning, machine weaving, coating and the like, greatly simplifies the technological process of forming the protective clothing fabric, and enables industrialized production to be easily achieved.