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Visible-light-driven photocatalyst Ag-AgCl-BiOCl and preparation method and application thereof
InactiveCN105251515AIncrease profitHigh catalytic activityPhysical/chemical process catalystsWater/sewage treatment by irradiationCoprecipitationHigh electron
The invention belongs to the technical fields of water pollution treatment and photocatalytic materials, and particularly relates to a visible-light-driven photocatalyst Ag-AgCl-BiOCl and a preparation method and application thereof. The visible-light-driven photocatalyst is a composite photocatalyst which is obtained from AgCl and BiOCl through a coprecipitation method and denoted as Ag-AgCl-BiOCl. The preparation method comprises the steps that ethanediol and water are taken as solvent, the BiOCl-AgCl are subjected to coprecipitation in the solvent, precipitation is irradiated through a xenon lamp, part AgCl is resolved into Ag, and the Ag-AgCl-BiOCl composite photocatalyst is finally formed. The composite photocatalyst effectively increases absorption of the BiOCl within a visible light range, a larger specific surface area and the higher electron-hole separation efficiency are showed, and compared with pure BiOCl and AgCl, better adsorbability and photocatalytic activity are achieved. The visible-light-driven photocatalyst Ag-AgCl-BiOCl can be widely used for dye and pharmaceutical wastewater treatment.
Owner:FUDAN UNIV
Solenoid valve with multiple-part armature without armature guide
InactiveCN101990597AShort intervalSmall rebound tendencyMachines/enginesFuel injecting pumpsSolenoid valveEngineering
The invention relates to a solenoid valve (16, 36) for actuating a fuel injector (10). The latter comprises a valve piece (14), in which a control chamber (86) is formed. Its outlet (82) can be opened or closed by way of a closing element (22) which can be actuated via the solenoid valve (16, 36). An armature plate (18) is received in a hydraulically damped manner (72, 56, 73) on an armature pin (20) which has a central outlet (62).
Owner:ROBERT BOSCH GMBH
Intermediate image receiving sheet for a thermal transfer recording method
InactiveUS6475695B2Enhance the imageQuality improvementDiffusion transfer processesPhotography auxillary processesRough surfaceIntermediate image
Disclosed is provide an intermediate image receiving sheet for a laser thermal transfer recording method, which can easily transfer an image to even a rough surface paper with high quality and stable repeatablility, and can use a variety of papers to produce and image proof which yields results similar to a print made in a production run.
Owner:KONICA CORP
Three-arm polyethylene glycol derivative and preparation method thereof
ActiveCN104744685ASimple structureEasy to separateAntibody ingredientsPharmaceutical non-active ingredientsEpoxyEthylene oxide
The invention provides a three-arm polyethylene glycol derivative with a structure as shown in formula I. The three-arm polyethylene glycol derivative has a single functional group which is positioned on the center of a high polymer framework, and a product obtained by modifying a protein drug with the three-arm polyethylene glycol derivative is simple in structure, and convenient to separate and purify in the later stage. Besides, the three-arm polyethylene glycol derivative provided by the invention can perform site-specific modification on biological active molecules under a relatively gentle condition, is convenient to transform the functional group, relatively high in modification rate and capable of improving the activity of the biological drug and the in-vitro stability of the biological drug. Meanwhile, the three-arm polyethylene glycol derivative is stable in quality, and has stable repeatability on the modified biological drug. The invention further provides a preparation method of the three-arm polyethylene glycol derivative; an initiator with the structure as shown in formula II is adopted to process after performing ring opening polymerization on epoxy ethane to obtain the three-arm polyethylene glycol derivative.
Owner:吕常海
Arc end tooth addendum measuring method
InactiveCN105651225AImprove detection accuracyStable measurement data and repeatabilityMeasurement devicesPhysicsRectangular coordinates
An arc end tooth addendum measuring method comprises the following steps: using the scanning probe of a three-coordinate measuring machine to measure the addendum of an arc end tooth, and acquiring actual contour data points of a standard concave tooth at the reference circle of the end tooth through a cylindrical scanning function; establishing a rectangular coordinate system, measuring a datum plane A, determining a z-axis direction and an oxy plane, measuring a benchmark hole, setting the center of the hole as the origin of coordinates o, and taking any direction as an x-axis direction; and setting end tooth measurement parameters, selecting Edit scanning data in the three-coordinate measuring machine, and setting selected unknown circumferential scanning. By using the arc end tooth addendum measuring method, the detection precision of standard arc end tooth addendum is improved.
Owner:SHENYANG LIMING AERO-ENGINE GROUP CORPORATION
Super-hydrophobic chitosan oil-absorbing sponge and preparation method thereof
InactiveCN109485911ATightly boundCombined with automaticOther chemical processesAlcoholFreeze-drying
The invention relates to a preparation method for super-hydrophobic chitosan oil-absorbing sponge. The preparation method comprises the following steps: (1) preparing chitosan-based sponge with a three-dimensional porous structure by using a vacuum freeze-drying method; (2) preparing modified Fe3O4 particles, and enabling the modified Fe3O4 particles and nano zinc oxide particles to be loaded in the three-dimensional porous structure of the chitosan-based sponge; and (3) soaking the obtained chitosan-based sponge in octadecanethiol-absolute ethyl alcohol solution, and repeatedly compressing and expanding, after curing, to obtain a product. Compared with existing chitosan-based sponge, the product has excellent hydrophobic oil-absorbing properties, and a high repeated utilization ratio.
Owner:湖北彩砼新材料有限公司
Method for realizing efficient differentiation of induced pluripotent stem cells towards mature endothelial cells
ActiveCN108384746ASignificant in vitro tube forming abilityReduce distractionsArtificial cell constructsArtificially induced pluripotent cellsGerm layerProgenitor
The invention discloses a method for realizing efficient differentiation of induced pluripotent stem cells towards mature endothelial cells, belonging to the technical field of cell engineering. The method comprises the following steps: carrying out pretreatment culturing on the induced pluripotent stem cells; carrying out induction to form side plate mesoblastic cells; carrying out inducing to form endothelial progenitor cells tending to the endothelial cells; and carrying out inducing to form the mature endothelial cells. According to the method, various cell inducing factors are matched through increase and decrease, meanwhile, the reasonable upgrading is carried out on the inducing method, thus the directional differentiation of the induced pluripotent stem cells towards the endothelial cells can be efficiently induced, under the condition that a small number of induced pluripotent stem cells are adopted and the purifying operation is not needed, a large number of mature endothelial cells with high purity and complete functions are obtained.
Owner:ALLIFE MEDICAL SCI & TECH CO LTD
Separating and purifying process of isochrysis galbana exopolysaccharide
The invention provides a separating and purifying process of isochrysis galbana exopolysaccharide; and the process comprises the following steps of: preparing crude isochrysis galbana exopolysaccharide by using isochrysis galbana culturing solution as a raw material; separating the crude isochrysis galbana exopolysaccharide ECPS by ion exchange chromatography, so as to obtain neutral polysaccharide and acidic polysaccharide components; loading the neutral polysaccharide and acidic polysaccharide components on a gel column for chromatography, so as to obtain eight components, wherein except for an ECPSII-A component, the quantities of the rest polysaccharide components are small; purifying the ECPSII-A component by using the gel column chromatography, so as to obtain a single polysaccharide component; and depositing, freezing and drying the single polysaccharide component by ethanol so as to prepare pure exopolysaccharide ECPSIII. According to the invention, the separating and purifying process of the isochrysis galbana exopolysaccharide is established; and the process is simple and reasonable and has the advantages of stable reproducibility and good operability. A good experiment basis is laid for follow-up study on biological activity of the polysaccharide; moreover, the microalgae polysaccharide can be applied to the medicine and medical field like other polysaccharide substances.
Owner:HUAIHAI INST OF TECH
Preparation of precise resistance/strain sensitive conductive silicon rubber
InactiveCN101412852AGood electrical conductivity and ductilityEasy to prepareForce measurementElectrical/magnetic solid deformation measurementHigh conductivityRubber material
The invention discloses a method for preparing a strain sensitivity-conductivity silicone rubber for a precise resistor and relates to a conductivity-strain sensitivity rubber material with low modulus and high repeatability. The preparation method comprises the following steps: the surface of a high-conductivity nanometer ruthenium oxide powder body is subjected to hydrophilization treatment; the surface of the nanometer ruthenium oxide powder body is modified through a silane coupling agent of which a terminal group is an ethylene group to form the conductive ruthenium oxide powder body containing the ethylene group on the surface; the conductive ruthenium oxide powder body is mixed with silicone rubber; the volume percentage of the ruthenium oxide powder body accounts for 17 to 30 percent of the total volume of the mixture; the mixture is added with a vulcanizing agent and a strengthening agent, is mixed, is kept stand for 30 minutes, is put to a mould, is pressurized to a pressure of between 10 and 15 Mpa, is kept at a temperature of between 172 and 178 DEG C for 15 to 30 minutes to obtain the vulcanized silicone rubber composite material; and the conductivity of the vulcanized silicone rubber composite material has the characteristic of repeated change along strain.
Owner:SOUTHEAST UNIV
Apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips
InactiveCN104513866AStrong specificityIncreased sensitivityMicrobiological testing/measurementMicroorganism based processesDiseasePrunus
The present invention discloses an apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips. The nucleotide sequences of the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primer pair are shown as SEQ ID No.1 and SEQ ID No.2, and the nucleotide sequences of the probe is shown as SEQ ID No.3. The nucleotide sequences of the prunus necrotic ringspot ilarvirus detection specific primer pair are shown as SEQ ID No.4 and SEQ ID No.5, and the nucleotide sequences of the probe is shown as SEQ ID No.6. By combination of RT-PCR (reverse transcription-polymerase chain reaction) detection, the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection gene chips prepared by use of the probes are tested, results show good specificity, strong sensitivity, stable reproducibility, short operation time and simple operation, and the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection gene chips can be widely used in early diagnosis and prevention and control of plant virus disease caused by the apple stem grooving virus and the prunus necrotic ringspot ilarvirus.
Owner:BEIJING UNIV OF AGRI
Quantitative analysis method capable of reducing analytical apparatus spectrogram relative deviation RSD
InactiveCN101509885AReduce the relative deviation RSD valueReproducible and stableRadiation pyrometryComponent separationInternal standardPeak area
The invention discloses a quantitative analysis method which is capable of reducing the relative spectrogram deviation (RSD) of an analytical instrument. The method comprises the steps of preparation of a measuring solution, drawing of a measure curve and calculation of a measured value, wherein besides components to be measured, internal standard substance components are quantitatively added in the preparation of the solution to be measured; after measurement, a series of discrete signal points are obtained on a new coordinate system, and continuous curves of different types of equipment and corresponding methods are further obtained; and an internal standard normalized spectrogram defined in the method is obtained by calculating the spectrogram, and the calculated peak height or peak area is called relative peak height or relative peak area. The quantitative analysis method has the beneficial effects that as the new spectrogram expression method of internal standard normalized spectrogram is creatively designed and adopted for the first time, the RSD value of the spectrogram in the existing analytical instrument is greatly reduced, namely the influence of operating conditions on the peak height is obviously reduced; and proved by experiments, remarkable improvement effects are achieved in the existing various instrument analysis methods.
Owner:QIQIHAR MEDICAL UNIVERSITY
Quality control method of pummelo pee medicinal material
InactiveCN101559123AFast wayReproducible and stableMicrobiological testing/measurementPlant ingredientsQuality controlReal-time polymerase chain reaction
The invention provides a quality control method of a pummelo pee medicinal material based on gene level. The quality control method comprises the following steps: (1) extracting genome DNAs of a genuine pummelo pee medicinal material and a medicinal material to be detected; (2) taking the extracted genome DNAs as primers in sequence numbers of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 respectively to perform polymerase chain reaction; (3) respectively performing electrophoretic separation on products from the polymerase chain reaction by agarose gel electrophoresis; and (4) comparing electrophoretograms of the sample to be detected and the genuine pummelo pee medicinal material. The method has the advantages of good stability and repeatability, and helps obtain the genetic relation between the medicinal material to be detected and the genuine medicinal material, can help ensure selection of fine varieties based on the gene level and helps ensure the quality of medicinal materials from the source.
Owner:GUANGZHOU GREEN LIFE PHARMA
Epitaxial growth method of high-resistance GaN thin film
ActiveCN107887255AFree installationImprove economySemiconductor/solid-state device manufacturingHigh resistanceImpurity doping
The invention relates to an epitaxial growth method of a high-resistance GaN thin film. The method is performed in MOCVD equipment and includes a substrate baking stage, a nucleation stage and an epitaxial growth stage. The method is characterized in that a metal organic matter trimethylindium is used as a C impurity doping source during the epitaxial growth stage. With the method adopted, the high resistance of the GaN thin film can be achieved; the TMIn is unlikely to form In-N bonds in crystal lattices under high temperature, and therefore, high growth temperature can assist in avoiding theformation of InGaN alloys and ensuring the integrity of the lattice structure of the GaN thin film; the concentration of C impurities in a GaN epitaxial layer can be effectively controlled by changing the flow rate of the dopant TMIn, and therefore, doping efficiency is high, and repeatability is stable; and a doping source pipeline is not required to be added into an MOCVD system, other C dopingsources are not required to be installed, and therefore, the resources of existing device can be fully utilized. The method is of simplicity and easy to operate. The epitaxial material has good performance. The high-quality and low-cost growth of the high-resistance GaN thin film can be realized.
Owner:NO 55 INST CHINA ELECTRONIC SCI & TECHNOLOGYGROUP CO LTD
Lily virus gene chip and preparation method thereof as well as method for detecting lily viruses using lily virus gene chip
InactiveCN104451893AIncreased sensitivityStrong specificityNucleotide librariesMicrobiological testing/measurementGel electrophoresisVirus
The invention discloses a lily virus gene chip and a preparation method thereof as well as a method for detecting lily viruses using the lily virus gene chip. The lily virus gene chip includes a solid phase carrier and an oligonucleotide probe fixed on the carrier, wherein the probe is selected from nucleotide segments corresponding to one or more virus genes of lily symptomless virus (LSV), cucumber mosaic virus (CMV) or lily mottle virus (LMoV). The viruses detected by the lily virus gene chip provided by the invention include three types of viruses which have severe impact and damage on cut lily flowers. The method for detecting lily viruses using the lily virus gene chip, provided by the invention has the advantages that specific primers are used for PCR amplification; amplification products are fluorescence-labeled, with high accuracy; the gene chip is high in sensitivity; a probe with specificity is selected to hybridize with the amplification products, with high specificity; the defect that a solution is false positive when the PCR products are detected through gel electrophoresis can be avoided; the detection is fast; the method is good in stability and low in cost, and is applicable to an accurate detection on a large number of lily samples in short time.
Owner:BEIJING UNIV OF AGRI
Molecular culture medium for culturing small intestine organoids of pigs
ActiveCN112501111AReproducible and stableIn line with physiological and biochemical characteristicsGastrointestinal cellsCulture processNutritionIntestino-intestinal
The invention discloses a molecular culture medium for culturing small intestine organoids of pigs. The molecular culture medium consists of a WNR-J2 condition culture medium and a basal culture medium, and the volume percentage concentration of the WNR-J2 condition culture medium in the molecular culture medium is 50-60%, and the basic culture medium is composed of Advanced DMEM / F12, an HEPES buffer solution, a B27 serum substitute, gastrin I, N-acetyl-L-cysteine, niacinamide10M, L-glutamine, N2-MAX, ethanolamine and an EGF epidermal growth factor. The molecular culture medium is suitable forsmall intestine crypt separation and culture of suckling piglets, weaned piglets and fattening pigs, long-term passage and maintenance of the small intestine organs of the pigs and intestinal nutrition and disease modeling of the pigs.
Owner:INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
Muskmelon SSR molecular marker system and application thereof
ActiveCN108841989AHigh polymorphismClear bandMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceRepeatability
The invention discloses a muskmelon SSR molecular marker system and application thereof, and relates to the technical field of molecular markers. The names of primer pairs for identifying muskmelon varieties are C30, MU5499, MU5554-1, SSR12083 and SSR04219 respectively. The researched SSR molecular marker primer pairs have high polymorphism, clear stripes and stable repeatability, can be used forconstructing muskmelon DNA fingerprints and identifying the muskmelon varieties, especially identifying XizhouMi 1, XizhouMi 3, XizhouMi 25, XizhouMi 17, XizhouMi 21 or XizhouMi 29, and have a more obvious effect.
Owner:新疆维吾尔自治区葡萄瓜果研究所
<Pi>-type-conjugated oxidation reduction polymer modified lead storage battery and preparation method therefor
ActiveCN106920935AExtend discharge timeExtended service lifeLead-acid accumulatorsCell electrodesPolymer modifiedHigh rate
The invention relates to the field of a lead-acid storage battery, and discloses a <pi>-type-conjugated oxidation reduction polymer modified lead storage battery and a preparation method therefor. Positive electrode lead paste of the battery comprises 98.5-99.5 parts of lead powder, 0.2-0.8 parts of additive and 0.3-0.7 parts of mixed polymer powder; and the mixed polymer powder is prepared from a <pi>-type-conjugated oxidation reduction polymer, a surface anti-oxidation functional group sulfonated arene polymer and single-wall carbon nanotubes. The active material of the lead storage battery comprises the surface-modified and anti-oxidation functional group conjugated oxidation reduction polymers, so that strong electronic storage capability and rapid electronic reaction speed are achieved; the polymers comprise available oxidation reduction nodes, so that relatively strong oxidation reduction activity is achieved; therefore, the lead storage battery has relatively high energy density, and the discharging time of the battery is obviously prolonged in a high-rate discharging condition of the battery; and meanwhile, the service life of the battery is prolonged.
Owner:CHAOWEI POWER CO LTD
Ultrahigh performance liquid chromatography method for detecting aflatoxin B1 content and aflatoxin M1 content of livers, kidneys and chicken of broiler chickens simultaneously
InactiveCN106770789AImprove accuracyHigh sensitivityComponent separationQuantitative determinationBroiler chick
The invention discloses an ultrahigh performance liquid chromatography method for detecting the aflatoxin B1 content and the aflatoxin M1 content of livers, kidneys and chicken of a broiler chicken simultaneously, and belongs to the field of ultrahigh performance liquid chromatography detection on the aflatoxin content. The ultrahigh performance liquid chromatography method for detecting the aflatoxin B1 content and the aflatoxin M1 content of the livers, the kidneys and the chicken of the broiler chickens comprises the following steps: (1) extracting aflatoxin B1 and aflatoxin from liver, kidneys or chicken tissues of the to-be-detected broiler chicken to obtain extracting liquid; (2) purifying and deriving the extracting liquid; and (3) performing qualitative and quantitative determination by the ultrahigh performance liquid chromatography method. By the method provided by the invention, the aflatoxin B1 and the aflatoxin in the liver, the kidneys and the chicken of the broiler chicken can be extracted and separated sufficiently; and the method has the advantages of high accuracy degree, stable reproducibility, high sensitivity, quickness and the like, and can be applied to detection of aflatoxin residues in edible tissues of animals.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Memristor based on metalloporphyrin heterojunction, preparation method thereof and application
ActiveCN110289350ADifferentiate critical voltageReduce energy consumptionElectrical apparatusHeterojunctionMetal
The invention discloses a memristor based on a metalloporphyrin heterojunction, a preparation method thereof and application. The memristor comprises a bottom electrode, a heterojunction layer, a metal oxide layer and a top electrode arranged in sequence from bottom to top, wherein the heterojunction layer comprises two metalloporphyrin layers for regulating the electrical properties of the memristor; and the metal oxide layer is used for providing ions and vacancies required for the operation of the memristor. The metalloporphyrin heterojunction memristor has a simple preparation process, is stable in properties, is suitable for a flexible device, can be processed in a large area, has changeable and adjustable property indexes and has the voltage-dependent filtering characteristics; and besides, the device yield, the repetition and the property stability are high.
Owner:NANJING UNIV OF POSTS & TELECOMM
Shift-by-wire switch test device, system and method
InactiveCN106370406AMeet the test requirementsReproducible and stableMachine part testingRobotic armComputer module
The invention discloses a shift-by-wire switch test device, system and method based on a six-degree-of-freedom mechanical arm. The test device comprises a position information management system for establishing a space coordinate system for determining all working planes of the mechanical arm in a working area of the mechanical arm, a test module for sending a test instruction corresponding to a test case according to the test case selected by a user, a motion control module and a shooting module. The test module is also used for obtaining the gear, where a shift-by-wire switch locates, according to a position map, judging whether the gear obtained when the mechanical arm reaches a preset target position corresponding to the test instruction is consistent with the gear, where the shift-by-wire switch locates, and testing whether the shift-by-wire switch is qualified. The shift-by-wire switch test device, system and method comprise the six-degree-of-freedom mechanical arm and the test system corresponding to the six-degree-of-freedom mechanical arm; motion redundancy thereof can meet test requirements of a new shift-by-wire switch; and the shift-by-wire switch test device, system and method have stable repeatability and wide application range.
Owner:KOSTAL SHANGHAI MANAGEMENT +2
Preparation method of ambroxol hydrochloride impurity reference substance
ActiveCN109060473AImprove securityReduce experimental riskComponent separationPreparing sample for investigationChemistryDichloromethane
The invention discloses a preparation method of an ambroxol hydrochloride impurity reference substance. The method comprises the following steps: firstly, dissolving ambroxol hydrochloride in an appropriate amount of dichloromethane solution, then adding a quantitative aqueous solution of sodium hydrogencarbonate for uniform stirring to obtain free ambroxol, separating, drying and concentrating the organic phase, dissolving the concentrated solution in the alcohol solution, then refluxing with excess orthoformate to obtain a crude product of (1R, 4R)-4-(6,8-dibromo-3,4-dihydroquinazoline)-cyclohexanol after condensation, and obtaining a pure product after the crude product is purified by silica gel column chromatography. The invention has the creative application of orthoformate as a condensing agent. Compared with other condensing agents reported in the literature, the orthoformate has the advantages such as good stability, high safety, low cost, high product yield and easy purification.
Owner:SHANGLUO UNIV
Detection method of purity of 3-ethoxy-4-methoxybenzaldehyde
ActiveCN108008035AEfficient separationGood peak shapeComponent separationUltraviolet detectorsAcetonitrile
The invention discloses a detection method of purity of 3-ethoxy-4-methoxybenzaldehyde. In the detection method, a phenomenon chromatographic column is adopted, two mobile phases are adopted for gradient elution, a mobile phase A is KH2PO4 buffer solution, a mobile phase B is acetonitrile, and the flow velocity of the each mobile phase is 0.7-1.2mL / min; a detector is an ultraviolet detector. The detection method is simple, convenient, accurate, fast, reliable, highly repeatable and stable, the 3-ethoxy-4-methoxybenzaldehyde and known impurities thereof can be effectively separated, and the simple, convenient, accurate, fast and reliable detection method is provided for industrial production of the 3-ethoxy-4-methoxybenzaldehyde.
Owner:GUANGXI ENANTIOTECH PHARM CO LTD
Chinese medicinal preparation for treating diabetes and preparation method thereof
InactiveCN102048846APromote secretionAvoid side effectsMetabolism disorderPlant ingredientsWestern medicineDiabetes mellitus
The invention discloses a Chinese medicinal preparation for treating diabetes, which is prepared by the following steps of: extracting 1 to 100 parts of golden thread to obtain fine powder extract which contains golden thread polysaccharides, mixing the extract and 0.1 to 80 parts of pharmaceutical excipient, granulating, adding 0.1 to 30 parts of dried golden thread superfine powder, and pressing into tablets. The golden thread chewable tablets avoid side effects produced when patients with the diabetes take western medicines for a long time, are conveniently taken by the patients, improve the compliance of the patients, and are easily accepted by the patients, and particularly the advantages of the tablets are better reflected under the conditions of going out, traveling and the like.
Owner:TIANJIN PACIFIC PHARMA
A method for efficient differentiation of induced pluripotent stem cells into mature endothelial cells
ActiveCN108384746BShort timeImprove efficiencyArtificial cell constructsCell culture active agentsBiochemistryMesoblast
The invention discloses a method for realizing efficient differentiation of induced pluripotent stem cells towards mature endothelial cells, belonging to the technical field of cell engineering. The method comprises the following steps: carrying out pretreatment culturing on the induced pluripotent stem cells; carrying out induction to form side plate mesoblastic cells; carrying out inducing to form endothelial progenitor cells tending to the endothelial cells; and carrying out inducing to form the mature endothelial cells. According to the method, various cell inducing factors are matched through increase and decrease, meanwhile, the reasonable upgrading is carried out on the inducing method, thus the directional differentiation of the induced pluripotent stem cells towards the endothelial cells can be efficiently induced, under the condition that a small number of induced pluripotent stem cells are adopted and the purifying operation is not needed, a large number of mature endothelial cells with high purity and complete functions are obtained.
Owner:ALLIFE MEDICAL SCI & TECH CO LTD
Multifunctional pressure detecting and measuring switch
InactiveCN101881680BSimple structureVersatileFluid pressure measurementMagnetic/electric field switchesVacuum pressureEngineering
Owner:NANJING VOCATIONAL UNIV OF IND TECH
Recombinant canine sourced antibody scFv-Fc against H3N2 canine influenza virus
ActiveCN111704665AImprove featuresHigh purityImmunoglobulins against virusesAntiviralsCompetent cellTotal rna
The invention discloses a recombinant canine sourced antibody scFv-Fc against the H3N2 canine influenza virus (H3N2 CIV). The recombinant canine sourced antibody scFv-Fc is prepared by the following steps: extracting the total RNA of an infection cell and performing reverse transcription to obtain cDNA; amplifying VH, VL and Fc genes; performing PCR fusion, and extracting recombinant plasmids T-scFv and / or T-CE-Fc; performing amplifying to obtain scFv and / or Fc on the basis of the T-scFv and / or T-CE-Fc, linking the scFv and / or Fc target fragments into a cloning vector, and selecting a positivestrain; performing amplification by using the scFv as a template and CE-scFv-F and / or CE-scFv-R as a primer, and performing homogenous recombinant linkage on the product and the vector, transforminga Rosetta competent cell, and selecting a positive strain named ET-32a-scFv-Fc after resistance plate screening. The recombinant canine source antibody scFv-Fc can be used for preparing similar biological preparations. The recombinant canine sourced antibody against H3N2 sub-type CIV lays a material foundation for preventing and controlling canine infection caused by the H3N2 CIV and dealing withpotential menace of the H3N2 CIV on human health and public hygiene.
Owner:NANJING AGRICULTURAL UNIVERSITY
A kind of moisture detection method of silane paste
ActiveCN107421843BAchieve separationGuaranteed stabilityWeighing by removing componentLiquid statePhysical chemistry
The invention discloses a moisture detection method for silane paste. A sample to be detected is subjected to decompression and rotary evaporation to remove water by utilizing a rotary vacuum meter; the moisture detection method comprises the following steps: (1) weighing the weight of the sample to be detected and recording the weight; (2) setting the temperature of a water bath or an oil bath and controlling a rotary speed; (3) controlling relative air pressure in a flask to -110kPa to -81.2kPa; (4) carrying out the decompression and the rotary evaporation until the paste completely disappears and is melted into clarified liquid-state silane; (5) weighing the total weight of the flask and the sample to be detected and calculating the moisture content of the silane. The detection method disclosed by the invention is easy to popularize; the popularization of application of the silane paste and the construction quality control of the silane paste are facilitated; a the detection result has a small error and is accurate and reliable, and the repeatability of the result is very stable.
Owner:CCCC FOURTH HARBOR ENG CO LTD
High light transmittance, super tough, heat/solvent responsive repair polyurethane and preparation method thereof
ActiveCN113336919BExcellent self-healing performanceAchieving Resilience RepairPolymer scienceAlcohol
The invention discloses a high-transmittance super-strength and super-tough heat / solvent response repair polyurethane and a preparation method thereof. The heat / solvent responsive repairing polyurethane has a structural formula of 10≤n≤60, and is formed by polycondensation of isophorone diamine, toluene diisocyanate-terminated polypropylene glycol, isophorone diisocyanate and small molecule monohydric alcohol. The heat / solvent responsive repair polyurethane of the present invention can complete the repair process at room temperature or at 140°C with solvent assistance, and has high transparency and ultra-high toughness, up to 503MJ / m 3 , and the ability of rapid recovery, it can be stretched to 30 times its original length.
Owner:NANJING UNIV OF SCI & TECH
Solid Phase Extraction Analysis Method for Perfluorooctane Sulfonate in Wastewater
ActiveCN104977371BImprove representationReproducible and stableComponent separationLiquid chromatography mass spectroscopySolid phase extraction
The present invention discloses a solid-phase extraction analysis method of perfluorooctane sulfonic acid compounds in wastewater. The solid-phase extraction analysis method comprises: rinsing a solid-phase extraction column sequentially with 0.1% ammonium acetate-containing methanol, pure methanol and ultrapure water, and discarding the solutions after completing the rinsing; using a vacuum solid-phase extraction device to suck a wastewater sample, passing through the solid-phase extraction column, sequentially adding 2 mL of a solution containing acetone, acetonitrile and formic acid according to a ratio of 50:50:1, 3 mL of pure methanol and 3 mL of 0.1% ammonium acetate-containing methanol to carry out elution, and collecting and merging the eluent; blowing the eluent with nitrogen to approach a drying state, adding 1 mL of a solution containing methanol and water according to a ratio of 1:1, and uniformly mixing; and carrying out analysis testing through LC / MS / MS to obtain the result. According to the present invention, the sampling amount of the method is large, such that the samples have the good representativeness and can characterize the performance of the wastewater to the maximum extent; the operation process is simple and efficient, and the reproducibility of the laboratory data is stable; and the standard addition method recovery rate achieves 70%, the detection limit is low and is 0.01 [mu]g / L, the ecological requirements of the environment are met, and the tested compound variety is diverse.
Owner:深圳天祥质量技术服务有限公司
mip‑spme extraction fiber and extraction platform and application
ActiveCN105080515BUniform film thicknessReproducible and stableOther chemical processesComponent separationSolid-phase microextractionMetallic materials
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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