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Quality control method of pummelo pee medicinal material

A technology for transforming tangerine red and medicinal materials, which is applied in the field of quality control of tangerine red medicinal materials, and achieves the effects of stable repeatability, quality assurance, and rapid method.

Inactive Publication Date: 2009-10-21
GUANGZHOU GREEN LIFE PHARMA
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AI Technical Summary

Problems solved by technology

At present, large-scale GAP industrialization bases have emerged, and how to ensure the quality of medicinal materials is the key to ensuring the industrial production of orange reds. The existing technology identifies the quality of medicinal materials based on their appearance and the content of active ingredients, and controls them at the genetic level. The quality of raw medicinal materials has not been reported

Method used

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  • Quality control method of pummelo pee medicinal material
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  • Quality control method of pummelo pee medicinal material

Examples

Experimental program
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Effect test

Embodiment

[0044] Experimental instruments, reagents and materials

[0045] Instrument BS110-electronic balance (S, 0.1mg); Beijing Liuyi Instrument Factory DYY-6C electrophoresis instrument; Beckman-15CSR refrigerated high-speed centrifuge; PCR instrument (PE 2400); pure water instrument (Millipore Q-R); UVP GDS7600 Gel imager; TECAN Spectra FLUOR plus multifunctional microplate reader.

[0046] Reagent Sai Baisheng GoidVoew dye; Biowest agarose (regular); Shanghai Sangong SK2492 type PCR amplification kit; Shanghai Sangong random primer (sequence 1,2,3,4,5,6,7,8,9 in the sequence listing , 10 and 11); Shanghai Shenergy Gaming 3s column centrifugal plant genomic DNA kit.

[0047] Experimental Materials

[0048] Standard product: Daodihua Juhong (Maojuhong, Huazhou pomelo C. grandis 'Tomentosa') sample was collected from Laohuling (Test No. Sn5) in Huazhou Base, Guangdong, on 2005.04.21, and tested by Director Qiu Jinyu of Guangdong Institute of Traditional Chinese Medicine. Pharmacis...

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Abstract

The invention provides a quality control method of a pummelo pee medicinal material based on gene level. The quality control method comprises the following steps: (1) extracting genome DNAs of a genuine pummelo pee medicinal material and a medicinal material to be detected; (2) taking the extracted genome DNAs as primers in sequence numbers of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 respectively to perform polymerase chain reaction; (3) respectively performing electrophoretic separation on products from the polymerase chain reaction by agarose gel electrophoresis; and (4) comparing electrophoretograms of the sample to be detected and the genuine pummelo pee medicinal material. The method has the advantages of good stability and repeatability, and helps obtain the genetic relation between the medicinal material to be detected and the genuine medicinal material, can help ensure selection of fine varieties based on the gene level and helps ensure the quality of medicinal materials from the source.

Description

technical field [0001] The invention relates to a quality control method of medicinal materials, in particular to a quality control method of Huajuhong medicinal materials. Background technique [0002] RAPD (Random Amplified Polymorphic DNA), that is, random amplified polymorphic DNA technology, is a DNA molecular marker technology developed by Williams et al. and Welsh et al. in 1990. RAPD technology is based on PCR technology. It uses a series of oligonucleotide single strands with different randomly arranged base sequences as primers to amplify the genomic DNA under study with a single primer to complete DNA synthesis. By repeating the above process, amplified products with different fragment sizes can be produced, and many different bands can be obtained through electrophoresis separation and color development. The polymorphism of the amplified product fragments reflects the polymorphism of genomic DNA. According to the research of modern molecular biology, the divers...

Claims

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Application Information

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IPC IPC(8): A61K36/752C12Q1/68
Inventor 林励欧剑锋
Owner GUANGZHOU GREEN LIFE PHARMA
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