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Ultrahigh performance liquid chromatography method for detecting aflatoxin B1 content and aflatoxin M1 content of livers, kidneys and chicken of broiler chickens simultaneously

A technology of aflatoxin and ultra-high performance liquid phase, which is applied in the field of ultra-high performance liquid chromatography detection of aflatoxin content, can solve problems such as health threats, mutagenesis, and teratogenicity, and achieve high recovery rate, good accuracy, reproducible and stable effect

Inactive Publication Date: 2017-05-31
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After animals eat feed containing aflatoxin B1 (AFB1), they will produce aflatoxin M1 (AFM1) through hydroxylation metabolism in the body, both of which are extremely toxic and can cause human, especially children, various feeding Animal poisoning can also cause cancer, teratogenicity, and mutagenesis. Even if the amount of tens of micrograms per kilogram of the substance is still very toxic, both will remain in the edible parts of animals such as liver, kidney, and muscle. , poses a great threat to the health of humans, poultry, and livestock

Method used

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  • Ultrahigh performance liquid chromatography method for detecting aflatoxin B1 content and aflatoxin M1 content of livers, kidneys and chicken of broiler chickens simultaneously
  • Ultrahigh performance liquid chromatography method for detecting aflatoxin B1 content and aflatoxin M1 content of livers, kidneys and chicken of broiler chickens simultaneously
  • Ultrahigh performance liquid chromatography method for detecting aflatoxin B1 content and aflatoxin M1 content of livers, kidneys and chicken of broiler chickens simultaneously

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Detection of Aflatoxin B1 and M1 Content in Broiler Liver, Kidney and Chicken by Ultra High Performance Liquid Chromatography

[0028] 1. Experimental method

[0029] 1.1 Preparation of reagents

[0030] PBS solution preparation: Accurately weigh NaCl 8.0g, KCl 0.2g, KH 2 PO 4 0.2g, Na 2 HPO 4 .12H 2 Dissolve 2.9 g of O in about 800 mL of deionized water, adjust the pH to 7.4, adjust the volume to 1000 mL, and store in a refrigerator at 4°C.

[0031] Preparation of aflatoxin B1 standard substance: take a portion of Sigma-Aldrich 1mg packaged AFB1 standard substance, dissolve it in a brown volumetric flask with chromatographic grade methanol and dilute to 10ml, that is, 100μg / ml standard stock solution, -20°C Keep in the refrigerator for later use.

[0032] Preparation of aflatoxin M1 standard substance: Take 1ml of Sigma-Aldrich AFM1 standard solution with a concentration of 0.5μg / ml, and use chromatographic grade acetonitrile in a brown volumetric fla...

experiment example 1

[0097] Experimental example 1 condition optimization experiment

[0098] 1. Optimization of the extraction method

[0099] (1) Optimization of organic solvent for extraction

[0100] Take the blank tissue, add the standard amount of AFB1 to 0.02 μg / kg, and AFM1 to 0.01 μg / kg, respectively use dichloromethane, 70% methanol solution, 80% methanol solution, 90% methanol solution, pure methanol solution and 84% acetonitrile solution (volume percent) extraction, other extraction parameters and ultra-high performance liquid chromatography detection parameters are the same as in Example 1. According to the standard addition recovery analysis, when the extraction solvent was dichloromethane, the recovery rates of AFB1 and AFM1 were significantly higher than other extraction solvents (Table 13).

[0101] The impact of table 13 organic solvent on AFB1 and AFM1 recovery rate (%)

[0102]

[0103] Note: The recovery rate of the same aflatoxin in different extraction solvents is co...

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Abstract

The invention discloses an ultrahigh performance liquid chromatography method for detecting the aflatoxin B1 content and the aflatoxin M1 content of livers, kidneys and chicken of a broiler chicken simultaneously, and belongs to the field of ultrahigh performance liquid chromatography detection on the aflatoxin content. The ultrahigh performance liquid chromatography method for detecting the aflatoxin B1 content and the aflatoxin M1 content of the livers, the kidneys and the chicken of the broiler chickens comprises the following steps: (1) extracting aflatoxin B1 and aflatoxin from liver, kidneys or chicken tissues of the to-be-detected broiler chicken to obtain extracting liquid; (2) purifying and deriving the extracting liquid; and (3) performing qualitative and quantitative determination by the ultrahigh performance liquid chromatography method. By the method provided by the invention, the aflatoxin B1 and the aflatoxin in the liver, the kidneys and the chicken of the broiler chicken can be extracted and separated sufficiently; and the method has the advantages of high accuracy degree, stable reproducibility, high sensitivity, quickness and the like, and can be applied to detection of aflatoxin residues in edible tissues of animals.

Description

technical field [0001] The invention relates to a method for detecting the content of aflatoxin B1 and aflatoxin M1 in broiler liver, kidney and chicken tissue, in particular to an ultra-high performance liquid chromatography for simultaneously detecting the content of aflatoxin B1 and M1 in broiler liver, kidney and chicken tissue The method belongs to the field of ultra-high performance liquid chromatography detection of aflatoxin content. Background technique [0002] Aflatoxins (Aflatoxins, referred to as AFT) are a group of highly toxic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus, including aflatoxins B1, B2, G1, and G2, especially aflatoxin B1. Pollutants have a wide distribution range and are mainly produced and present in moldy and deteriorating grains or their products. After animals eat feed containing aflatoxin B1 (AFB1), they will produce aflatoxin M1 (AFM1) through hydroxylation metabolism in the body, both of whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张秀英金慧然崔晓旭李蕊郭文欣
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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