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Apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips

A technology of apple stem groove virus and ring spot virus, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of false positives, time-consuming, cumbersome operations, etc., and achieve stable reproducibility and easy operation Short-term, high-sensitivity effects

Inactive Publication Date: 2015-04-15
BEIJING UNIV OF AGRI
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Problems solved by technology

In practical application, some methods of these technologies have a cumbersome purification process and take a long time, and some operations are cumbersome, easy to cause pollution, and false positives

Method used

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  • Apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips
  • Apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips
  • Apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips

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Embodiment

[0022] 1 Test materials and methods

[0023] 1.1 Test material

[0024] Positive samples of apple leaves infected by apple stem groove virus ASGV were provided by the Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine. Cherry leaves infected by PNRSV were provided by Changli Fruit Tree Research Institute, Hebei Academy of Agriculture and Forestry Sciences. Negative samples are Cucumber Green Mottle Mosaic Virus (CGMMV), Potato Broom Top Virus (PMTV), Potato Yellow Dwarf Virus (PYDV), Broad Bean Wilt Virus (BBWV), Maize Rough Dwarf Virus (MRDV), Rice Stripe Virus (RSV) , pepper mild mottle virus (PMMoV), taro mosaic virus (DsMV), tomato mosaic virus (ToMV), hibiscus chlorotic ringspot virus (HCRSV), by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, China Agricultural University, Zhejiang Agricultural Provided by the Academy of Sciences and the Plant Protection Station of Tanghai County, Hebei Province.

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Abstract

The present invention discloses an apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primers and probes and gene chips. The nucleotide sequences of the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection specific primer pair are shown as SEQ ID No.1 and SEQ ID No.2, and the nucleotide sequences of the probe is shown as SEQ ID No.3. The nucleotide sequences of the prunus necrotic ringspot ilarvirus detection specific primer pair are shown as SEQ ID No.4 and SEQ ID No.5, and the nucleotide sequences of the probe is shown as SEQ ID No.6. By combination of RT-PCR (reverse transcription-polymerase chain reaction) detection, the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection gene chips prepared by use of the probes are tested, results show good specificity, strong sensitivity, stable reproducibility, short operation time and simple operation, and the apple stem grooving virus and prunus necrotic ringspot ilarvirus detection gene chips can be widely used in early diagnosis and prevention and control of plant virus disease caused by the apple stem grooving virus and the prunus necrotic ringspot ilarvirus.

Description

technical field [0001] The invention relates to primers and probes for detecting plant viruses and a gene detection chip prepared by using them, in particular to detecting apple stem groove virus (Apple stem grooving virus, ASGV) and Prunus necrotic ringspot virus (Prunus necrotic ringspot virus, PNRSV) The specific primers and probes and the gene chip prepared by using them belong to the field of molecular detection of apple stem groove virus and Prunus necrotic ringspot virus. Background technique [0002] Apple stem groove virus (Apple stem grooving virus, ASGV) is one of the most common latent viruses in apples and pears. It has been reported that the natural host of the virus can also harm fruit trees such as cherries, apricots, and citrus, as well as lily. Prunus necrotic ringspot virus (PNRSV) is a virus that is distributed worldwide. . PNRSV is a second-class entry quarantine pest in China, and it is a serious threat to fruit tree cultivation in the north. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/94
CPCC12Q1/70C12Q1/6837
Inventor 王进忠谷瑞华郝少东杨宝东张志勇王升启
Owner BEIJING UNIV OF AGRI
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