The invention discloses a method for extracting
genome DNA of
prawn white spot baculovirus. It takes the surviving sick
shrimp or fresh dead
shrimp suffering from white spot baculovirus
disease as samples, washes them with
sterile water first, then puts the samples in a heating vessel, adds
sterile water and immerses them to 2-3cm, and heats them. After boiling, keep for 8-10 minutes, add TN buffer and
grind to make a homogenate, and process through medium-speed
centrifugation and other steps to obtain WSSV
genomic DNA. Because the present invention adopts the method of heating and boiling to pretreat the sample, the sample is easily ground, and the homogenate is rapid and sufficient; also because after the sample is heated, the
animal protein, including the
viral protein, coagulates and denatures, and the nucleocapsid of the
virus particle is easy to separate from the tissue, The
DNA yield is greatly improved; at the same time, for the
early detection of diseased
shrimp such as seedlings, whole shrimp can also be used, which facilitates the acquisition of samples. The invention provides a fast, convenient and effective way for extracting WSSV
genome DNA.