Portable fluorescence microscope based on smart phone and working method thereof

Abstract

The invention provides a portable fluorescence microscope based on a smart phone and a working method thereof. The portable fluorescence microscope based on a smart phone comprises a smart phone and aportable fluorescence microscope arranged on the smart phone. The portable fluorescence microscope comprises a fluorescence excitation light source, a collimating lens, a light filter group and an objective lens. The collimating lens is perpendicular to the light emitted by the fluorescence excitation light source. The light filter group is parallel to the collimating lens. The objective lens isperpendicular to the collimating lens. The objective lens is arranged before a sample to be detected. The built-in lens of the smart phone is parallel to the objective lens. The fluorescence excitation light source irradiates the sample to be detected after passing through the collimating lens and the light filter group. The reflected light of the sample to be detected is received by the built-inlens of the smart phone via the light filter group. By using the portable fluorescence microscope of the invention, the microscopic sample fluorescence analysis experiment is greatly simplified, and the high requirement of the conventional microscope for the experimental environments and conditions is lowered. The portable fluorescence microscope is a practical application of the Internet technology and intelligent communication in basic scientific research.

Description

technical field [0001] The invention belongs to the field of microscopes, and in particular relates to a portable fluorescent microscope based on a smart phone and a working method thereof. Background technique [0002] Fluorescence microscopy is an effective tool for observing the absorption and transport of microscopic substances and the distribution and localization of certain chemical substances. The commercial fluorescence microscope system is relatively complex, including high-pressure mercury lamp light source, imaging equipment, excitation filter, two-color beam splitter, blocking filter and some focusing auxiliary devices, etc. The overall size of the equipment is generally tens of centimeters, and the price is about Tens of thousands of RMB, poor portability. In recent years, along with the continuous development of smartphone built-in imaging systems and processor-based image processing performance, as well as the continuous improvement of mobile phone App softwa...

AI Technical Summary

Problems solved by technology

The commercial fluorescence microscope system is relatively complex, including high-pressure mercury lamp light source, imaging equipment, excitation filter, two-color beam splitter, blocking filter and some focusing auxiliary devices, etc. The overall size of the equipment is generally tens of centimeters, and the price is about Tens of thousands of RMB, poor portability

Images