The invention relates to a detachable and reusable hydrophobic or super-hydrophobic microfluidic organ chip, which utilizes a hydrophobic or super-hydrophobic surface with low critical surface tension to construct a microfluidic organ chip, and specifically can construct a heart chip, a liver chip, a brain chip, and a brain chip. Chip, tumor chip, kidney chip, gut chip, skin chip, fat chip, blood vessel chip, uterus chip, eye chip, nose chip, bone chip, periodontal chip, islet chip, spleen chip, placenta chip, lung chip, muscle chip Chips, Throat Chips, Bone Marrow Chips, Diabetes Chips, and Multi-Organ Chips. The organ chip constructed by the invention is detachable and reusable, thereby greatly reducing the application cost of the microfluidic organ chip.