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Detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips

An organ chip and microfluidic technology, which is applied in biochemical instruments, tissue cell/virus culture devices, and stress-stimulated microbial growth methods, etc., can solve problems such as complex procedures, high cost of microfluidic organ chips, and impact on use.

Active Publication Date: 2021-01-29
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the vigorous academic research, the development of the microfluidic organ chip industry is still relatively lagging behind. One of the main problems is that the traditional microfluidic organ chip is mainly based on polydimethylsiloxane (PDMS) elastic materials, and processing requires the use of light. The engraving technology is complex, the process is lengthy, and the PDMS chip can only be used for a limited number of times (in most cases, it is used once), resulting in a high cost of the microfluidic organ chip itself.
[0004] The main reason why the PDMS microfluidic organ chip is not durable is that the microchannels and chambers in the PDMS microfluidic organ chip are a closed micron-scale space. In addition, the surface adsorption effect of PDMS is very serious, and it is difficult to remove the inside after one use. Embedded cells, three-dimensional glue and other substances are cleaned, which will affect the second use

Method used

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  • Detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips
  • Detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips
  • Detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: A reusable heart chip based on a hydrophobic surface

[0081] The heart is the blood-supplying organ of the human body, mainly composed of cardiomyocytes ( Cardiomyocytes ), cardiac fibroblasts ( Fibroblast ), vascular endothelial cells ( Endothelial cells ) and macrophages ( Macrophage ). The heart chip is an in vitro model of the heart, which is used to investigate the toxicity or efficacy of drugs on the heart. In toxicity or efficacy evaluation experiments, it is often necessary to measure the changes of different types of cell proteins and genes. Traditional heart chips often do this for bionic purposes. Several types of cells are mixed cultured, and after administration, it is difficult to separate these different types of cells for gene and protein detection. Therefore, the present invention proposes a heart chip that is especially suitable for proteome and genome detection.

[0082] Such as Image 6 As shown in (A) and (B), the heart chip is ...

Embodiment 2

[0085] Example 2: A reusable liver chip based on a hydrophobic surface

[0086] The liver is the largest metabolic organ in the body, mainly composed of hepatic parenchymal cells ( Hepatocytes ),Fibroblasts( Fibroblasts ), stellate cells ( Stellate cells ), liver vascular endothelial cells ( Endothelial cells ), bile duct epithelial cells ( Biliary epithelial cells ) and Kupffer cells ( Kuppfer Cells )Wait. The liver chip is an in vitro model of the liver, which is used to investigate the metabolism of drugs in the liver and the toxicity of drugs to the liver. In the metabolism and toxicity evaluation experiments, it is necessary to measure the changes of different types of cells at the protein and gene levels. The traditional liver These types of cells are often mixed for bionicity. After administration, it is difficult to separate these types of cells for detection of genes and proteins. Therefore, the present invention proposes a chip that is especially suitable ...

Embodiment 3

[0090] Example 3: A reusable tumor chip based on a hydrophobic surface

[0091] Tumor is one of the major human diseases, and tumor tissue mainly contains tumor cells ( Cancer cells ),Fibroblasts( Fibroblasts ), vascular endothelial cells ( Endothelial cells ) and immune cells ( Immune cells )Wait. The tumor chip is an in vitro model of tumors, used to investigate the anti-tumor activity of drugs. In drug efficacy evaluation experiments, it is necessary to measure the changes of different types of cells at the protein and gene levels. Traditional tumor chips often use these types of cells for bionic purposes. After administration of mixed culture, it is difficult to separate these different types of cells for gene and protein detection. Therefore, the present invention proposes a tumor chip that is especially suitable for proteome and genome detection.

[0092] The structure of the tumor chip is the same as that of the heart chip in Example 1, but the cells cultured in...

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Abstract

The invention relates to detachable and reusable hydrophobic or super-hydrophobic micro-fluidic organ chips, which are constructed by using a hydrophobic or super-hydrophobic surface with low criticalsurface tension. Specifically, a heart chip, a liver chip, a brain chip, a tumor chip, a kidney chip, an intestinal chip, a skin chip, a fat chip, a blood vessel chip, a uterus chip, an eye chip, a nose chip, a bone chip, a periodontal chip, a pancreas islet chip, a spleen chip, a placenta chip, a lung chip, a muscle chip, a throat chip, a bone marrow chip, a diabetes chip and a multi-organ chipare constructed. The organ chips constructed by the method are detachable and can be repeatedly used, so that the application cost of the micro-fluidic organ chips is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a detachable and reusable hydrophobic or superhydrophobic microfluidic organ chip. Background technique [0002] Microfluidic organ chip is a cutting-edge emerging technology, which refers to the co-cultivation of a variety of mammalian cells in a microfluidic chip to control the three-dimensional spatial arrangement of cells, fluid shear force and signal molecule concentration, simulating real Organ microenvironment, technology to realize real organ function. In 2016, the World Davos Conference selected it as one of the "Top Ten Emerging Technologies" in the world, and it is believed that it can affect the lives of human beings in the future. [0003] The microfluidic organ chip has been developed for nearly 10 years, and it has entered the stage of industrialization at this stage. In China, there have been specialized microfluidic organ chip companies that have begu...

Claims

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Application Information

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IPC IPC(8): C12M3/06C12M3/00
CPCC12M23/16C12M23/20C12M29/04C12M35/08
Inventor 张秀莉丛烨罗勇
Owner SUZHOU UNIV
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