Sterile air-permeable sealing film structure for microbe culture bottle

Abstract

The invention relates to a sterile air-permeable sealing film structure for a microbe culture bottle. The structure can be flexibly regulated and adapted according to size specifications of different culture bottles; the upper part of an adapted culture bottle is provided with a bottle neck; a plastic film is bound by a binding tape or rubber band at the bottle neck of the culture bottle; a bottle neck seal is correspondingly placed at the opening of the bottle neck; the central position of the bottle neck seal is provided with a sealing film air-permeable window structure; the sealing film air-permeable window structure comprises a bottom layer film and a top layer film; a filtering medium is arranged between the bottom layer film and the top layer film; and the central positions of the upper and lower surfaces of the filtering medium are fixedly connected with the bottom layer film and the top layer film through center dispensing. The sealing structure can effectively filter and obstruct microbes in the air, and simultaneously can ensure the supply of the air in the culture bottle. Besides, the sealing structure can effectively prevent the filtering medium from producing a leak due to dislocation or folding deformation in the culture process, thereby ensuring the culture safety. The sealing structure is simple and convenient to use, hardly occupies the volume inside / outside the bottle, and improves the culture efficiency.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation equipment, and in particular relates to a structure of a sterile air-permeable sealing film of a microorganism cultivation bottle. Background technique [0002] In the process of microbial cultivation, culture bottles are needed, and the culture bottles usually need to be sealed to prevent microorganisms such as bacteria and fungi from entering the bottle and causing pollution. At present, the commonly used sealing method is to directly bind film, kraft paper or gauze, cotton and chemical fiber spherical plugs on the bottle mouth. There are several defects in the following sealing methods: (1) gauze, Although cotton and chemical fiber spherical plugs can meet the air permeability requirements, the plugs are easy to absorb moisture and be contaminated with bacteria, and the plugs are relatively large and troublesome to operate, requiring a certain amount of space inside and outsid...

AI Technical Summary

Problems solved by technology

At present, the commonly used sealing method is to directly bind film, kraft paper or gauze, cotton and chemical fiber spherical plugs on the bottle mouth. There are several defects in the following sealing methods: (1) gauze, Cotton and chemical fiber spherical plugs can meet the air permeability requirements, but the plugs are easy to absorb moisture and contaminate bacteria, and the plugs are large and troublesome to operate, and need to occupy a certain amount of space inside and outside the bottle mouth

(2) The sealing operation of polypropylene, polyethylene film or kraft paper is simple, does not occupy the space inside and outside the bottle mouth, but does not have good air permeability, which affects the oxygen supply and growth of the bacteria, and the bottle mouth sealing film after sterilization is often due to the internal and external pressure difference. However, expansion and deformation occur, and kraft paper is prone to cracking under the conditions of humidity, heat and pressure difference.

(3) A kind of sealing film currently designed and tested adopts a three-layer structure, and a layer of filter paper is set as a filter medium in the interlayer of two layers of plastic films, and four symmetrical air holes are processed on the upper and lower surfaces of the two layers of plastic films to ensure ventilation , but during use, due to changes in the air pressure inside the culture bottle or uneven pulling during the sealing operation, displacement or wrinkle deformation will occur between the filter paper and the film at the bottle mouth, so that the air entering the culture bottle is not filtered. Filtration and direct entry from the gaps in the vent holes will not achieve the purpose of filtering bacteria, fungi and other external microorganisms, resulting in the risk of contamination of the internal culture. In addition, because the upper and lower plastic films are not well fixed, it is easy to As a result, the upper and lower symmetrical air holes do not correspond to each other, which cannot ensure the smooth flow of air, thus affecting the effect of cultivating air supply

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