71results about How to "Improve the effect of clinical application" patented technology

The invention provides a novel biological-material complex patch which is of a laminated structure comprising a basic layer and an additional layer. The novel biological-material complex patch is characterized in that the basic layer is an patch made from an artificial synthetic material; the additional layer is a biological patch made from an extracellular matrix / acellular tissue matrix material, or a synthetic-material patch made from an artificial synthetic biological material or the artificial synthetic biological material and an artificial synthetic degradable material; and the basic layer and the additional layer are integrated through a surgical suture or a medical adhesive or other methods. The biological-material complex patch has the advantages of easiness for peripheral tissue growth and angiogenesis, adhesion prevention, degradability, good infection resistance and the like of the biological material and the advantages of easiness for tissue growth by of a big mesh, excellent infection resistance, difficulty for generating hydrops, non-degradability, high late mechanical strength and durability of the artificial-synthetic-material patch, adopts a simple production method, is more effective after being applied in clinic, and ensures recent and late relapse and infection rates to be lower.

The invention provides a quality control method for a Chinese medicinal capsule for treating anemofrigid cold. The Chinese medicinal capsule is prepared from bulk drugs such as schizonepeta spike, mulberry leaf, divaricate saposhnikovia root, radix bupleuri, incised notopterygium rhizome, kudzuvine root, platycodon root, bitter apricot seed, dahurian angelica root, bunge corydalis, reed rhizome and the like, and can be prepared by adding accessories according to a conventional process. The quality control method for the Chinese medicinal composition comprises thin layer qualitative check for the schizonepeta spike, the kudzuvine root, the bunge corydalis, the radix bupleuri, the divaricate saposhnikovia root and the platycodon root, and quantitative detection for puerarin, cimiside and 4'-O-glucopyranosyl-5-O-methylvisamminol. The Chinese medicinal composition has obvious effect of treating the anemofrigid cold.

A crystallized cefathiamidine is prepared from the cefathiamidine solution through controlling pH to 3.5-6.5, adding proper solvent until the solution becomes cloudy, controlling saturation procedureand crystal growth speed while adding said solvent, separating and drying. Its advantage is uniform granularity. It can be used to prepare the medicines for treating the infection of staphylococcus aureus, pneumoccus, enterococcus, streptococcus, etc.

The invention relates to ceftizoxime sodium composition sterile powder for injection. The powder contains 99.0 to 99.9 weight percent of ceftizoxime sodium and 0.10 to 1.00 weight percent of sodium benzoate, wherein the ceftizoxime sodium is in a crystallized form. As for the ceftizoxime sodium in the crystallized form, the temperature of solution of ceftizoxime sodium is controlled by adjusting the pH value of the solution and the saturation process of the solution is controlled by a method of adding appropriate amounts of different solvents into the solution in multiple times so as to obtain a ceftizoxime sodium crystal form with uniform grain size distribution. A method for preparing the powder has simple, convenient and easily-controlled production process and low preparation cost and the obtained ceftizoxime sodium in the crystallized form has uniform grain size distribution and is more stable than the ceftizoxime sodium in the market.

The invention discloses an antimicrobial peptide XYZ-1. The antimicrobial peptide XYZ-1 is a straight-chain peptide, and contains 19 amino acid residues, the molecular weight is 2288.81Da, the isoelectric point is 10.83, and the amino acid sequence of the antimicrobial peptide XYZ-1 is arginine-glycine-glycine-arginine-leucine-cysteine-tyrosine-cysteine-arginine-arginine-arginine-proline-cysteine-valine-cysteine-valine-glycine-arginine-lysine (SEQ ID NO.1). The antimicrobial peptide XYZ-1 disclosed by the invention does not produce hemolysis, the antimicrobial spectrum is wide, the antimicrobial peptide XYZ-1 has a highly effective antimicrobial effect on gram-positive bacteria, gram-negative bacteria and fungi, and therefore can be applied perfectly in the preparation of drugs for treating gram-positive bacterium, gram-negative bacterium or / and fungus disease infections, the clinical application effect of the antimicrobial peptide XYZ-1 is better, and moreover, the antimicrobial peptide XYZ-1 can also be used in the preparation of feed additives or food additives.

The invention relates to fast check paper of Staphylococcus aureus causing food poisoning, in particular to a formula of a fast check reagent of food poisoning caused by the intestinotoxin of toxin-production Staphylococcus aureus and a preparation method thereof. The invention breaks through a traditional sectional check means and utilizes the characteristics of heat-resisting desoxyribonucleic acid and plasma-coagulase, the check diagnosis process is shortened to 8-12 hours, and the identification accuracy is larger than 97 percent. The fast check paper has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check paper has an application value, has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines, and has an obvious advantage in the application of rapidly identifying and diagnosing toxin-production Staphylococcus aureus which is a food poisoning pathogenic bacterium.

The invention discloses a duodenal papilla incision knife. A knife body is shaped like a long and thin strip, can freely penetrate through a biopsy hole of a gastroscope and is provided with a hollow wire penetrating channel, and one steel wire can be inserted into the wire penetrating channel; the front end of the knife body is provided with a section of a knife head, the front section of the steel wire penetrates out of the wire penetrating channel and then is embedded in the knife head, the knife head is supported at the front end of the knife body through the front section of the steel wire, and the steel wire can rotate to drive the knife head to rotate; the tail end of the knife head is provided with a wire pulling guide seat, and the wire pulling guide seat is provided with a wire feeding hole; the knife body is further provided with a thin metal wire in an embedded mode, the front section of the thin metal wire penetrates out of the front end of the knife body and then penetrates into the wire feeding hole, and the head end of the front section of the thin metal wire is embedded into the front end of the knife head. The duodenal papilla incision knife has the advantages of being novel in structure, more flexible in operation, higher in practicability and more ideal in clinical application effect.

The invention discloses an F9 gene copy number variation detection kit which comprises a 2*PCR (Polymerase China Reaction) buffer solution, competitive DNA, PCR primer mixed liquor and Taq DNA polymerase. An AccuCopy technology has high precision, variable coefficient of being less than 5% and high result accuracy. Therefore, according to the F9 gene copy number variation detection kit developed by the AccuCopy technology, about 15 patients suffering hemophilia B with large deletion mutation is detected and found, and the clinical application effect is good.

The invention relates to a method for preparing fermented traditional Chinese medicinal fever-clearing and toxicity-eliminating powder. The method comprises the following steps: (1) weighing the traditional Chinese medicinal materials according to a proportion, grinding and uniformly mixing, and screening for later use; (2) inoculating a strain in a liquid culture medium, and culturing in a shaker at 20-40 DEG C for 18-24 hours to obtain a fermented strain for later use, wherein the strain is one or more than two of bacillus subtilis, saccharomyces cerevisiae and lactobacillus bulgaricus; (3) sterilizing the traditional Chinese medicinal powder obtained in the step (1) at 110-130 DEG C for 10-30 minutes, uniformly mixing the fermented strain and sterile water, and inoculating the mixture into the traditional Chinese medicinal powder, performing anaerobic fermentation culture for 2-7 days at 20-40 DEG C, stirring once every 12 hours, and drying the fermented product after the fermentation culture is finished. Clinical tests discover that the fermented traditional Chinese medicinal fever-clearing and toxicity-eliminating powder has a good effect on preventing and treating pestilent maculae and hyperpyretic coma, is remarkable in curative effect, convenient to use, small in toxic and side effect and green and has no residue.

The present invention provides a Chinese medicine with the functions of promoting blood circulation, removing blood stasis, astringing sore and subduing swelling for preventing bed sore with obvious effect. Said Chinese medicine is made up by using 9 Chinese medicinal materials of anomalous Artemisia, biota leaf, rose-of Sharon root bark, coptis root, aloe and others and vegetable oil through a certain preparation process.

The invention relates to a preparation method for compound clotrimazole, betamethasone valerate and gentamicin sulphate suspended ear drops for treating ear inflammation of dogs. According to the preparation method, the above several medicines are stably and uniformly stored in oily ear drops and not precipitated in several hours, with stable effective ingredient contents through a special process treatment. The production process disclosed by the invention is simpler and more convenient to operate, easily available in raw materials, beneficial to industrialized production, good in stability, convenient to store and transport, and remarkable in clinic treatment effect, as well has a large-scale production and clinic application advantage.

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

The invention discloses a medicament for treating gonarthromeningitis, which is prepared from raw materials comprising Kusnezoff monkshood roots, rhizoma arisaematis, roots of common peony, dried gingers, cinnamon, angelica roots, semen coicis, honeysuckles, poria peel, medicinal cyathula roots, rhizome of Chinese atractylode and bighead atractylodes rhizome. The medicament can be prepared into various preparations for clinical use, has a good curative effect on gonarthromeningitis and has the advantages of no obvious toxic or side effect and has safe and reliable clinical use.

The invention relates to an egg yolk culture medium for amino acid neutralization, in particular to a prescription of a culture medium used when a gonorrhea patient need the gonococcus culture examination after using a large amount of clinical antibiotics and a preparation method of the culture medium. The gonococcus culture examine is an important method used for diagnosing gonorrhea. The purulent inflammation which is the disease of genitourinary system and caused by gonococcus is one of the pathogens to which clinical doctors attach importance. Especially when the large amount of clinical antibiotics is used, the gonococcus detection is very difficult because a sample contains a large amount of residual antibiotics, and the recovered gonococcus culture is still influenced by the residual antibiotics, therefore the design mistake of not adopting an experiment for removing the residual antibiotics is very serious, and the result obtained by the experiment is not reliable. The invention aims to provides an egg yolk culture medium for amino acid neutralization, which is used for clinically judging the gonococcus used in the sample of a genitourinary tract.

The invention relates to a traditional Chinese medicine for treating gallstone, and in particular relates to a traditional Chinese medicine for treating damp-heat type gallstone, which is prepared from the following components in parts by weight: 9-12 parts of chingma abutilon seed, 25-30 parts of stringy stonecrop, 12-15 parts of grifola, 20-30 parts of longhairy antenoron herb, 6-9 parts of cape jasmine fruit, 15-20 parts of abrus cantoniensis hance, 6-9 parts of golden cypress, 3-6 parts of herba aristolochiae, 3-6 parts of rhizoma nardostachyos, 6-9 parts of fructus aurantii, 6-9 parts of pericarpium citri reticulatae viride, 15-20 parts of verbena, 6-10 parts of whiteflower cacalia seed, 9-12 parts of radix curcumae,0.5-3 parts of barbados aloe and 6-12 parts of areca-nut. In the formula, abrus cantoniensis hance, pericarpium citri reticulatae viride, radix curcumae and cape jasmine fruit can sooth liver-gallbladder, clear heat, cool blood and remove stagnation; chingma abutilon seed, grifola, longhairy antenoron herb and herba aristolochiae can clear dampness, promote diuresis, clear heat and remove toxicity; longhairy antenoron herb and radix curcumae have a function of digesting gallstone; fructus aurantii and areca-nut serve as aids to depress qi and regulate qi, excrete water and excrete dyspepsia; stringy stonecrop, golden cypress, verbena and whiteflower cacalia seed can discharge fire, promote diuresis, eliminate stasis and subdue swelling; and therefore, functions of clearing liver-gallbladder, digesting and discharging gallstone can be enhanced. Meanwhile, rhizoma nardostachyos can promote appetite and refresh spleen, and barbados aloe can clear liver heat, so that spleen is tonified, liver is relived, and unfavourable causes of the liver and the gallbladder are eliminated. The traditional Chinese medicine has the advantages of having an effective rate of 100%, taking effect quickly, treating both symptoms and root causes and being safe and reliable.

The invention discloses a preparation method of a titanium-based tantalum coating biological implant material. A titanium-based substrate of which a rough surface is formed by sand blasting treatmentand tantalum metal are placed in a vapor deposition reaction chamber, chlorine and hydrogen are injected to the reaction chamber for a vapor deposition reaction, the vapor deposition time is 7-10 h, and the reaction temperature of vapor deposition is 900-1000 DEG C. According to the preparation method of the titanium-based tantalum coating biological implant material, a tantalum coating obtained by the deposition has good morphology, the thickness of the tantalum coating can be up to 20 microns, and the tantalum coating is uniform and dense; the binding force between the coating and the substrate is greater than 40 Mpa, and the requirements of anti-stripping in the clinical application process can be met; and the prepared titanium-based tantalum coating biological implant material has theaverage compression resistance strength of 611+ / -20.4 Mpa, the yield strength of 508+ / -15.8 Mpa and the elastic modulus of 102+ / -4.1 Gpa, and good bend resistance strength and yield strength are achieved.

The invention provides a follicular granulosa cell anti-senescence technology, wherein animal follicular granulosa cells are employed for transplantation, the process comprises the steps of (1) placing ovarium of pig or sheep into curling, (2) culturing the follicular granulose cells, shearing ovarium into small blocks, carrying out grinding, sieving, slaking, purifying and placing into culture bottle, then charging QM-1640 culture liquid containing AB-serum, loading into CO2 incubator for culturing, (3) the application method comprises injecting the ovarian follicle granular cells into human body muscle or waist subarachnoid cavity.

The invention relates to a fast check reagent of Salmonella causing neutralization food source type food poisoning, in particular to a fast diagnosis reagent for a patient with most common Salmonella causing food source type food poisoning after a plurality of antibiotics are clinically used and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum and a neutralizing agent are added in enriched liquid, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the neutralizing agent can neutralize a plurality of residual antibiotics or disinfectors contained in a specimen and generate a neutralized product having no influence on the growth of Salmonella. The identification accuracy is larger than 99 percent, the fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria; and the check diagnosis process is shortened to 2-6 hours.

The invention relates to a carmustine sustained-release implant for treating solid tumor and a preparation method thereof. The implant is characterized in that the sustained-release implant comprises a sustained-release auxiliary material and an effective anti-tumor component carmustine, wherein the sustained-release auxiliary material is a lactide-glycolide copolymer, the viscosity range of the copolymer is 0.3-0.6, the weight percentage of carmustine is 7.5-30% and the mass proportion of lactide to glycolide is 50: 50. The invention further relates to a method for preparing the sustained-release implant and an application of the implant for treating solid tumors such as pancreatic cancer, lung cancer, liver cancer, breast cancer, brain tumor, esophagus cancer, renal carcinoma, colorectal cancer and the like, liver and brain metastatic tumors. The sustained-release period of the implant is 3-4 weeks, wherein the accumulated release in previous two days is 5-12% of the total drug loading capacity and the accumulated release in previous 12 days is greater than 40% of the total drug loading capacity but less than 60% of the total drug loading capacity.

The invention discloses a medicinal composition for nursing or preventing and treating tristimania, which relates to the field of a traditional Chinese medicine technology. The medicinal composition is prepared from the following traditional Chinese medicinal materials in parts by weight: 18-25 parts of radix bupleuri, 25-35 parts of fructus aurantii immaturus, 25-35 parts of semen ziziphi spinosae, 25-35 parts of folium mori, 15-22 parts of radix ophiopogonis, 15-22 parts of fructus gardeniae and 6-12 parts of polygala tenuifolia. The medicinal composition provided by the invention is capable of soothing liver-qi stagnation and dredging effects of the heart and the kidney by bringing a multi-target effect into play, and the clinical application effect is obvious.

A crystallized cefathiamidine is prepared from the cefathiamidine solution through controlling pH to 3.5-6.5, adding proper solvent until the solution becomes cloudy, controlling saturation procedure and crystal growth speed while adding said solvent, separating and drying. Its advantage is uniform granularity. It can be used to prepare the medicines for treating the infection of staphylococcus aureus, pneumoccus, enterococcus, streptococcus, etc.

The invention relates to a mycoplasma quick detection reagent for non-gonococcal urethritis, in particular to a quick detection test paper for macoplasma hominics and mycoplasma hominis and a preparation method thereof. The quick detection reagent can detect out the positive result of the mycoplasma in a genital tract, thereby having the function of the clinical diagnosis guidance and the clinical treatment and the significant meaning of preventing the abuse of antibacterial drugs. The mycoplasma quick detection reagent for the non-gonococcal urethritis is rich in nutrition and easy to prepare, achieves satisfied clinical application effect, solves the difficulty of bedside inoculation, is less polluted, has simple detection method, short time, strong selectivity, sensitive color change, high detection rate and good stability and can obtain a liquid for filtration enrichment and achieve the effects of permanently observing the growth condition of a colony after a sample is inoculated for 24 hours.

The invention discloses a quick-solidification anti-collapse dental filling material, and belongs to the field of biomedical materials. The dental filling material comprises a solid phase component and a liquid phase component, wherein the solid phase comprises at least one of calcium silicate compound, a quick-solidification anti-collapse agent and an X-ray retardant; the liquid phase is an aqueous phase based on distilled water or deionized water. In the quick-solidification anti-collapse dental filling material, disodium hydrogen phosphate powder is used as a quick-solidification anti-collapse agent. The composite material not only has excellent anti-collapse performance, but also has short solidification time, can induce hydroxyapatite deposition, and forms close fit with the root canal wall. Compared with prior materials, the material of the invention has the characteristics of rapid solidification, excellent anti-collapse property, edge sealing property, biological activity and the like.

The invention discloses a detection kit for F11gene copy number variation. The detection kit comprises 2*PCR buffer liquor, competitive DNA, PCR primer mixed liquor and Taq DNA polymerase. Only a few base differences are provided between a target gene segment and internal contrast DNA in the target gene segment, so that amplification efficiency of two templates shows high consistency, and therefore, final amplification products truly reflect concentration proportion of two templates before amplification. According to pretest results, standard deviation for repeating one competitive PCR reaction by three times is within 5%; moreover, if the target gene segment and the internal contrast DNA are mixed according to different dilution gradients to carry out competitive PCR, dependency of a ratio of a sample peak to an internal contrast peak area and an original concentration ratio of the two templates is over 99.9%. And therefore, the detection kit for F11gene copy number variation developed based on the principle and the technology is quick, high in accuracy and the like, and has a good clinical value.

The invention relates to a Nutritional digestive juice for chlamydia neutralization, in particular to a prescription of a cell culture medium used when a urethritis patient needs the examination of the chlamydia cell culture after using a large amount of clinical antibiotics and a preparation method of the cell culture medium. The genital tract chlamydia trachomatis infection is one of the most common diseases which are sexually transmitted. Chlamydia trachomatis is also regarded as the important cause of the pelvic inflammatory disease and the sequelae (tubal infertility and ectopic pregnancy) thereof. The chlamydia cell culture method is the most sensitive and the most reliable method used for examining the chlamydia. The culture medium can detect out the positive result of the genital tract chlamydia after the large amount of clinical antibiotics is used, thereby having the function of the clinical diagnosis guidance and the clinical treatment and the important meaning of preventing the abuse of the antibacterial drugs.

The invention relates to test paper for genital tract mycoplasma detection, in particular to a prescription of test paper for genital tract mycoplasma detection and a preparation method thereof. A quick detection reagent can detect out the positive result of the genital tract mycoplasma, thereby having the function of the clinical diagnosis guidance and the clinical treatment and the significant meaning of preventing the abuse of antibacterial drugs. The test paper for genital tract mycoplasma detection is rich in nutrition and easy to prepare, achieves satisfied clinical application effect, solves the difficulty of bedside inoculation, is less polluted, has simple detection method, short time, strong selectivity, sensitive color change, high detection rate and good stability and can obtain a liquid for filtration enrichment, achieve the effects of permanently observing the growth condition of a colony after a sample is inoculated for 24 hours and obtain a pure colony adhered thereon.

The invention relates to a quick detection test paper for ureaplasma urealyticum (uu) neutralization, in particular to a quick detection test paper used for uu neutralization after a large amount of clinical antibiotics is used and a preparation method thereof. The quick detection test paper for neutralization can detect out the positive result of genital tract mycoplasma after the large amount of clinical antibiotics is used, thereby having the function of the clinical diagnosis guidance and the clinical treatmen and the significant meaning of preventing the abuse of antibacterial drugs is. The quick detection test paper for uu neutralization is rich in nutrition and easy to prepare, has satisfied clinical application effect, solves the difficulty of bedside inoculation, is less polluted, has simple detection method, short time, strong selectivity, sensitive color change, high detection rate and good stability, can obtain a liquid for filtration enrichment and achieve the effects of permanently observing the growth condition of a colony after a sample is inoculated for 24 hours and can practically and reliably neutralize the corresponding clinical antibiotics; the products of reaction of the quick detection test paper for uu neutralization and the antibiotics do not sterilize experimental uu but inhibit the growth of the experimental uu; and the quick detection test paper for uu neutralization can rapidly and accurately detect the uu after a large amount of clinical antibiotics is used.

The invention discloses a traditional Chinese medicine composition for treating after-chemotherapy thrombocytopenia and relates to the field of traditional Chinese medicine and pharmacy. The composition is prepared from, by weight, 20-30 parts of processed radix astragali, 10-20 parts of donkey-hide gelatin, 20-30 parts of pyrola corbieri, 20-30 parts of agrimonia pilosa ledeb, 20-30 parts of cornu bubali, 9-15 parts of Rumex japonicus Meisn and 3-5 parts of peanut skin. The seven effective raw materials in the formula are easy to obtain and moderate in price and can meet the consumption level of most patients. The patients spend a lot in treating blood diseases, the composition in the formula is used for assisting treatment and has the effects of invigorating the spleen and tonifying the kidney, benefiting qi and nourishing blood and stopping bleeding, the composition aims to treat disease from the root, treat both symptoms and root causes and take both symptom treatment and root cause treatment into consideration, the effects of increasing blood platelet and reducing bleeding accidents are achieved, the treatment effect is reliable, the price is low, no toxic and side effects are caused, the clinical application effect is remarkable, and economic burden of the patients is greatly reduced.

The invention discloses a bacteria transfer and preservation culture medium used in the transfer process and a preparation method thereof. The culture medium comprises the following components: beef extract, peptone, sodium chloride, agar, glycerol, aluminum potassium sulfate, malt extract powder, yeast extract powder, ferric ammonium sulfate, calcium lactate, D-cycloserine, argutone, vitamins, casein phosphopeptides, glucose, lanthanum nitrate, erythritol, sterile defiberized sheep blood and distilled water. The preparation method comprises the steps: adding corresponding parts of culture medium components into the corresponding distilled water, dissolving, uniformly mixing, sterilizing at a high pressure, sub-packaging, and preserving and transferring bacteria in the transfer process through the culture medium. The physiological characteristics of a to-be-detected bacterial sample can be well maintained to be basically unchanged, the effects of enriching bacteria and preventing infectious microbe pollution can be further achieved, and compared with a conventional culture medium, the culture medium disclosed by the invention is high in detection rate, low in pollution, satisfied in clinical application effect and good in transfer effect; and the detection positive rate of the microbial sample is greatly improved.

The invention discloses nucleic acid sequences and a kit for detecting 19-Del mutation of an epidermal growth factor receptor (EGFR) gene and belongs to the technical field of molecular diagnosis. Theprimers, the probes and the kit for detecting 19-Del mutation of the EGFR gene can detect 53 common mutation types (covering 99% of mutation occurrence rate or above) of EGFR-TKIs targeting drugs forclinical NSCLC patients with high sensitivity and high specificity. The invention also discloses the kit for detecting 19-Del mutation of the EGFR gene. The kit can realize quantitative detection ofmutation of 19-Del of the EGFR gene in one hole conveniently, quickly, sensitively, specifically and accurately, can be used as the reference basis of EGFR-TKIs targeting drugs and can be used for dynamically monitoring curative effects of drugs in real time.