Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Egg yolk culture medium for amino acid neutralization

An amino acid and medium technology, applied in the field of neutralizing amino acid yolk medium, can solve problems such as unreliable results and design errors, and achieve the effect of sensitive method, high accuracy and high sensitivity

Inactive Publication Date: 2009-10-28
曲奕
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, experiments that do not use measures to remove residual antibiotics should be considered as serious mistakes in design, and the results based on such experiments are not reliable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Below in conjunction with embodiment the present invention is described in further detail:

[0013] Configure according to the data listed in the table below (grams, ml / liter of distilled water) and the steps described:

[0014] Recipe one:

[0015] Amino acid growth-promoting liquid (containing 8 kinds of amino acids such as cystamine, glutamic acid, arginine, methionine, proline, aspartic acid, serine, isoleucine, cystine and cysteine, hypoxanthine, Uracil 2 bases,) 2ml; polyvalent peptone 9.0-12.0; peptone 4.0-5.0; tryptone 4.0-5.0; yeast extract powder 4.0-6.0; glucose 1.0-2.0; soluble starch 1.5-2.5; Sodium chloride 14.0-16.0; dipotassium hydrogen phosphate 3.0-5.0; potassium dihydrogen phosphate 1.5-2.5; agar powder 14.0-16.0; 2.5mg / L polymyxin B 0.2-0.3ml; 3mg / L vancomycin 0.2 -0.3ml; 15% sterile egg yolk saline 10.0-15.0ml; soil temperature -800.3-0.5; lecithin 0.5-0.8; double distilled water 1000ml, pH7.4.

[0016] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an egg yolk culture medium for amino acid neutralization, in particular to a prescription of a culture medium used when a gonorrhea patient need the gonococcus culture examination after using a large amount of clinical antibiotics and a preparation method of the culture medium. The gonococcus culture examine is an important method used for diagnosing gonorrhea. The purulent inflammation which is the disease of genitourinary system and caused by gonococcus is one of the pathogens to which clinical doctors attach importance. Especially when the large amount of clinical antibiotics is used, the gonococcus detection is very difficult because a sample contains a large amount of residual antibiotics, and the recovered gonococcus culture is still influenced by the residual antibiotics, therefore the design mistake of not adopting an experiment for removing the residual antibiotics is very serious, and the result obtained by the experiment is not reliable. The invention aims to provides an egg yolk culture medium for amino acid neutralization, which is used for clinically judging the gonococcus used in the sample of a genitourinary tract.

Description

technical field [0001] The invention relates to a neutralizing amino acid egg yolk culture medium, especially the formula and preparation method of the culture medium for gonorrhea patients who need gonococcal culture and inspection after a large amount of clinical antibiotics are used. The main ingredients of its formula are: amino acid growth-promoting liquid (containing 8 kinds of cystamine, glutamic acid, arginine, methionine, proline, aspartic acid, serine, isoleucine, cystine and cysteine) Amino acid, hypoxanthine, uracil two kinds of bases), polyvalent peptone, peptone, tryptone, yeast extract powder, glucose, soluble starch, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, Agar powder, polymyxin B, vancomycin, egg yolk, Tween-80, lecithin. Gonorrhea culture examination is an important method for the diagnosis of gonorrhea. Especially for chronic patients and female patients, it is necessary to make a correct diagnosis after being cultu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/04
Inventor 曲奕
Owner 曲奕
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com