The invention discloses a high-temperature-resistant, high-strength and beryllium-copper-free wire and a preparation method thereof. The preparation method comprises steps: S1, high-purity copper, pure iron and a block-shaped covering agent are mixed, molten, kept at the constant temperature and stirred, a copper-nickel alloy, a copper-strontium alloy, a copper-indium alloy, magnesium rods and rare earth element lanthanum are added, the mixture is heated to 1,230-1,260 DEG C, stirred and molten, and copper alloy melt is obtained; S2, component detection is performed on the copper alloy melt; S3, the copper alloy melt obtained in S2 is introduced into a high-vacuum single-roll rotary quenching and spray casting system and heated to 950-970 DEG C for spray casting, air is introduced after cooling, and a thin strip is obtained after balance of atmospheric pressure; S4, the thin strip is subjected to continuous annealing and homogenization in a magnetic field under the condition of 590-630 DEG C, and the high-temperature-resistant, high-strength and beryllium-copper-free wire is obtained through cold rolling and ageing treatment. With the iron-copper alloy as a basis, multiple alloy elements are added in a matched manner to replace conventional beryllium copper alloy materials, the cost is reduced, a reasonable preparation process is matched, and the comprehensive performance of the material is improved.