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Application of rapamycin in inhibiting growth of penicillium citrinum

A technology of Penicillium citrus and rapamycin, which is applied in the application, preservation of food ingredients as antimicrobials, preservation of fruits and vegetables, etc., can solve the problems of toxicity, easy residue and high concentration.

Pending Publication Date: 2022-06-03
眉山职业技术学院(眉山技师学院)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemical antiseptic preservatives all have varying degrees of toxicity, and the concentration is large, easy to remain, and easy to pollute, which does not meet the needs of today's consumers for green and healthy fruits.

Method used

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  • Application of rapamycin in inhibiting growth of penicillium citrinum
  • Application of rapamycin in inhibiting growth of penicillium citrinum
  • Application of rapamycin in inhibiting growth of penicillium citrinum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Determination of the growth inhibition of Penicillium italy

[0053] The concentration gradient of rapamycin was set to 0.01 μmol / L, 0.1 μmol / L, and 1 μmol / L. Prepare PDA medium containing these three different concentrations of rapamycin, DMSO control and blank control respectively, under sterile conditions, drop 5 μl of spores in the center of each PDA plate with a concentration of about 10 6 CFU / ml mold spore suspension, 5 replicates for each treatment. After the bacterial solution was dry, the plate was sealed and cultured at 28±1°C for 7 days. The diameter of the plaque was measured by the cross method every day, and the antibacterial diameter was finally calculated.

[0054] Observing the mold growth conditions under different treatments, it can be found that the PDA medium containing three different concentrations of rapamycin has a certain inhibitory effect on the growth of Penicillium italia ( figure 1 ). The growth of molds in each plate on the 7...

Embodiment 2

[0055] Example 2. Determination of the growth inhibition of Penicillium italia spores

[0056] The concentration gradient of rapamycin was set to 0.01 μmol / L, 0.1 μmol / L, and 1 μmol / L. Prepare the PDA culture medium, DMSO control and blank control containing these 3 kinds of rapamycin of different concentrations respectively, under aseptic conditions, drop 1ml of about 20CFU / mL mold spore bacteria suspension on each PDA plate respectively, Use a coating bar to coat evenly with 5 replicates per treatment. After the bacterial liquid was dry, the plate was sealed and incubated at 28±1°C for 72 hours, then the colony was counted, and the colony diameter was measured by the cross method.

[0057] Observing the Italian mold spore growth status under different treatments can find that there is a certain amount of colony formation on each PDA flat plate ( Figure 4 ); Counting the colonies on each plate found that there was no significant difference in the number of colonies between...

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Abstract

The invention relates to novel application of rapamycin, in particular to application of rapamycin in inhibiting growth of penicillium italicum, specifically, 0.01 mu mol / L rapamycin has a relatively strong inhibiting effect on growth of penicillium italicum, and the inhibiting effect on growth of the penicillium italicum is increased along with increase of the concentration of the rapamycin. According to the invention, the TOR protein specific inhibitor rapamycin is used for preventing and treating the citrus penicilliosis for the first time. Researches find that the low-concentration rapamycin can effectively inhibit the growth rate of penicillium italicum, a new choice is provided for preventing and treating citrus penicilliosis, and a very significant basis is provided for selection research of new drugs and compound reagents of citrus preservatives; the method has important potential application value in the aspects of reduction of use of harmful chemical preservatives and green and sustainable development of citrus storage and preservation.

Description

technical field [0001] The invention relates to a new application of rapamycin, in particular to the application of rapamycin in inhibiting the growth of Penicillium citrus. Background technique [0002] Reference [1] In my country, citrus is one of the pillar industries of the rural economy and an important fruit tree crop cultivated in the southern region. Literature [2] pointed out that the yield of citrus has been increasing in recent years, but diseases such as penicillium, green mold, stalk rot, black rot, anthracnose and other diseases are very prone to occur in the post-harvest citrus storage and transportation process, resulting in serious economic losses. loss. Among them, penicillium and green mildew are the most common diseases of citrus during storage, and they are also the most harmful diseases. Literature [3] pointed out that 90% of the rot of citrus after harvest is caused by them, and literature [4] can cause the loss of citrus. The rate reaches 30% to 50%...

Claims

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Application Information

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IPC IPC(8): A23B7/155
CPCA23B7/155A23V2002/00A23V2200/10
Inventor 陈婷婷
Owner 眉山职业技术学院(眉山技师学院)
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