PDCD11-shRNA and application of PDCD11-shRNA in treatment of colorectal cancer
A colorectal cancer and treatment target technology, applied in the field of biomedicine, can solve the problems of lack of targeted drugs and limited targets, and achieve the effect of inhibiting growth rate, huge social and economic benefits, and broad market application prospects
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[0022] The technical scheme of the present invention is described in further detail below in conjunction with the examples: the present embodiment implements under the premise of the technical scheme of the present invention, and provides detailed implementation and specific operation process, but the protection authority of the present invention does not Limited to the following examples.
[0023] The source of material involved in the embodiment:
[0024] TE buffer (pH=8.0) was purchased from Beijing Soleibao Technology Co., Ltd.; trypsin, Opti-MEM ® I medium and DMEM medium were purchased from Gibco; Lipofectamine 2000 was purchased from Invitrogen; restriction endonuclease Age I. Eco RI and Bam HI was purchased from NEB Company; T4 DNA ligase was purchased from Nanjing Novizan Biotechnology Co., Ltd.; Escherichia coli DH5α was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; Lenti-X 293T cells were purchased from Clontech Company; puromycin was purcha...
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