PDCD11-shRNA and application of PDCD11-shRNA in treatment of colorectal cancer

A colorectal cancer and treatment target technology, applied in the field of biomedicine, can solve the problems of lack of targeted drugs and limited targets, and achieve the effect of inhibiting growth rate, huge social and economic benefits, and broad market application prospects

Active Publication Date: 2020-07-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the available targeted therapy targets are limited at this stage, and the available targeted drugs are scarce, and only a very small number of patients can benefit from targeted therapy. Therefore, providing a new type of tumor therapeutic target is an urgent problem to be solved

Method used

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  • PDCD11-shRNA and application of PDCD11-shRNA in treatment of colorectal cancer
  • PDCD11-shRNA and application of PDCD11-shRNA in treatment of colorectal cancer
  • PDCD11-shRNA and application of PDCD11-shRNA in treatment of colorectal cancer

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Embodiment Construction

[0022] The technical scheme of the present invention is described in further detail below in conjunction with the examples: the present embodiment implements under the premise of the technical scheme of the present invention, and provides detailed implementation and specific operation process, but the protection authority of the present invention does not Limited to the following examples.

[0023] The source of material involved in the embodiment:

[0024] TE buffer (pH=8.0) was purchased from Beijing Soleibao Technology Co., Ltd.; trypsin, Opti-MEM ® I medium and DMEM medium were purchased from Gibco; Lipofectamine 2000 was purchased from Invitrogen; restriction endonuclease Age I. Eco RI and Bam HI was purchased from NEB Company; T4 DNA ligase was purchased from Nanjing Novizan Biotechnology Co., Ltd.; Escherichia coli DH5α was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; Lenti-X 293T cells were purchased from Clontech Company; puromycin was purcha...

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Abstract

The invention provides PDCD11-shRNA. The PDCD11-shRNA is a shRNA molecule which is designed according to a gene sequence of nucleolin PDCD11 and targets to PDCD11; and the PDCD11-shRNA can remarkablyinhibit the growth of human colorectal cancer cells HCT116. The sequence of the PDCD11-shRNA disclosed by the invention is as shown in SEQ ID No. 1. The PDCD11-shRNA of the invention has the advantages that the expression level of PDCD11 in HCT116 tumor cells can be remarkably reduced, and the proliferation rate of the cells is remarkably inhibited. Therefore, the shPDCD11 molecule designed by theinvention has the potential of treating colorectal cancer.

Description

technical field [0001] The invention relates to a PDCD11-shRNA and its application as a therapeutic target for colorectal cancer, the classification number is C12N15 / 113, and belongs to the technical field of biomedicine. Background technique [0002] Colorectal cancer is currently one of the most common malignant tumors of the digestive tract, which seriously threatens human life and health. Its incidence rate ranks in the forefront of common malignant tumors, and its case fatality rate also ranks in the forefront of cancer deaths. The occurrence and development of colorectal cancer is a multi-step, multi-stage and multi-gene process, in which the abnormal expression of genes plays an important role in the development of colon cancer. Therefore, it is of great scientific significance to find new therapeutic targets for colorectal cancer to treat the disease and reduce the mortality rate of colorectal cancer. [0003] Targeted tumor therapy is to design and develop targeted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61P35/00
CPCC12N15/1135A61K31/713A61P35/00C12N2310/531C12N2310/14
Inventor 张新跃张杰丁笠陈磊张智萍刘雅娴聂也森何妍之
Owner YANGZHOU UNIV
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