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Shewanella spp and application thereof in microbial fuel cell
InactiveCN101838622AHas electricity-generating propertiesHigh electroactivityBacteriaCell electrodesShewanella putrefaciensBiology
The invention discloses shewanella spp and application thereof in a microbial fuel cell and relates to the technical field of biology. The shewanella spp is shewanella xiamenensis sp.nov.S4 preserved in the 'China Center for Type Culture Collection' in January, 2009 with the preservation number of CCTCC M 209017. The shewanella spp has the characteristics of negative Gram stain, rod-shaped and straight thallus, two circular ends, diameter of 0.3 to 1.2mu m, length of 2.5 to 5.8mu m, single or paired existence and short-chain arrangement, single flagella on the thallus, mobility, capability of producing electricity in a seawater environment and higher electricity production activity and is obtained by separating marine sediments near the sea.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Automatized continuous processing equipment for T shaped corner extrusion press and its processing method
InactiveCN101342547AUniform tissueContinuous extrusionExtrusion diesRams/plungersControl systemEngineering
The invention discloses a tee corner extruding automatic continuous processing equipment and a processing method thereof, relating to the field of material processing, particularly relating to a tee corner extruding equipment which can automatically and continuously process and produce superfine crystals or nanocrystalline metal-base materials and a method thereof. The invention includes a mould, a first punch, a second punch, a third punch and a feeding and discharging opening. Tee corner extruding cavities are arranged in three neighboring directions in the mould, the first punch, the second bunch and the third punch are arranged in the three cavities respectively; the feeding and discharging opening is arranged in the direction of the other side; the cross-section size of the feeding and discharging opening is larger than the first punch, the second punch and the third punch; the first punch, the second punch and the third punch are connected with a hydraulic control system respectively. The invention is simple in structure, easy to be operated and has an extensive application scope.
Owner:HOHAI UNIV
Metal surface nano-composite processing device with stirring head capable of synchronously feeding powder, and method
InactiveCN102560472AMaintain integrityImprove processing efficiencyMetallic material coating processesMetal substrateRotational axis
The invention relates to a processing device for synthesizing a surface layer of a nano-composite material in situ by performing friction stir processing (FSP) on a metal workpiece through a stirring head capable of synchronously feeding powder, and a method. A clamping part 1, a shaft shoulder 2 and a stirring probe 3 of the stirring head can be a rigid whole body or a fastening coaxial separated body; a powder feeding channel A is arranged in the stirring head; the inlet of the channel A is positioned on the side or middle axial position of the clamping part 1; and the outlet of the channelA is positioned on the surface of the middle axial position of the probe 3, which is contacted with the workpiece. During processing, a hydraulic device in a worktable capable of automatically lifting is used for pressurizing along the axial direction B of the stirring head, a rotating shaft drives the stirring head to rotate at high speed and press the workpiece, an external automatic powder feeder feeds sub-micron reinforcing phase powder into a seriously plasticized area C on the surface layer of the workpiece through the powder feeding channel A of the stirring head, and the nano-composite material is synthesized in situ through friction heat. The device is simple, is convenient to operate, is suitable for the composition and structure refining of various metal substrates, and is highin technical and economic benefit and industrial application potential.
Owner:HOHAI UNIV
Shewanella and application thereof in microbiological fuel cell
InactiveCN101880638AHas electricity-generating propertiesWide salt ion concentration toleranceBacteriaCell electrodesGram stain negativeMicroorganism
The invention discloses shewanella and application thereof in a microbiological fuel cell, and relates to the technical field of biology. The shewanella is Shewanella marisflavi EP1 which is preserved in 'China Center for Type Culture Collection' in January 2009 with the preservation number of CCTCC M 209016. The shewanella is characterized in that: the shewanella is gram-negative; the thalli are rod-shaped and straight; two ends are circular; the diameter is 0.3 to 1.0 micro; the length is 2.5 to 6.0 micros; the thalli can singly exist or are arranged in pair or in a short-chain shape; and the thalli have autogenetic flagella and can move. The shewanella is separated from offshore ocean sediments, has wide salt ion concentration tolerance activity, and can generate power in electrode liquid at the concentration of up to 8 percent. Therefore, the internal resistance of the microbiological fuel cell is effectively reduced.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Preparation method for graphene
InactiveCN105347332AIncrease path lengthIncrease shearing and grinding time and frequencyDefective grapheneSolvent
The invention provides a preparation method for graphene. The preparation method comprises: S1, dispersing natural graphite in a solvent to obtain a graphite suspension; S2, shearing the graphite suspension in a colloid mill; and S3, carrying out centrifugal separation on the sheared graphite suspension to obtain a supernatant, and carrying out vacuum suction filtration and drying on the supernatant to obtain graphene. By using the natural graphite powders which are wide in raw material source and cutting the graphite with the colloid mill, graphite granules can be effectively stripped through a physical action of a severe shear force, a frictional force, high-frequency vibration and high-speed vortex, so that the production efficiency is greatly improved and the graphene can be industrially prepared in batches. Meanwhile, the prepared graphene is few in defect, high in yield, low in cost, good in dispersity and high in quality, and the application range of the graphene is greatly expanded.
Owner:SHANDONG UNIV OF SCI & TECH
Gordona terrae C-6 and its desulfurization effect
InactiveCN1699547AGreat potential for industrial applicationsImprove featuresBacteriaFermentationBacterial strainGordona terrae
The invention relates to a Gordona terrae C-6 and its desulfurization effect, wherein the name of the bacterium is Gordona terrae C-6, it has been preserved in China General Microbiological Culture Collection Center (CGMCC), the collection number being CGMCC NO.1361. The bacterial strain is obtained through isolating from oil-water polluted soil around high-sulfur oil wells, and can be serve as catalyst for removing sulfur atoms in benzothiophene types sulfur-containing organic compounds, especially suitable for the removal of benzothiophenes organic heterocyclic sulfur in fossil fuels such as coal, oil and related products.
Owner:NANKAI UNIV
Tert-butyl asymmetric alpha-diimine nickel complex for preparation of ultrahigh-molecular-weight polyethylene elastomers and preparation method and application of tert-butyl asymmetric alpha-diimine nickel complex
ActiveCN108794545AHigh catalytic activityLow costNickel organic compoundsBulk chemical productionElastomerPolyolefin
The invention provides a tert-butyl asymmetric alpha-diimine nickel complex and an intermediate, a preparation method and application thereof. The prepared nickel complex has a single catalytic activecenter, regulation of polymer molecular weight and branching degrees can be realized through changing of a ligand structure and polymerization conditions, and high catalytic activity, low cost, performance stability and the like are achieved. The preparation method is mild in condition, short in period and simple in operation condition. The nickel complex can be applied to catalysts for vinyl polymerization, the catalytic activity is up to 1.26*10<7>g mol<-1>(Ni) h<-1>, the weight-average molecular weight Mw of prepared polyethylene fluctuates in a range of 1.0-30.8*10<5>g mol<-1>, molecularweight distribution is in a range of 1.9-5.0, the remarkable performance of polyethylene molecular weight control is achieved, the nickel complex can be used for preparation of ultrahigh-molecular-weight polyethylene, and obtained polyethylene is high in branching degree and is potential polyolefin elastomer.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Nickel sulfide/carbon nano tube flexible composite thin-film material and preparation method and application thereof
InactiveCN109873127AThe preparation process is stableReduce manufacturing costCell electrodesSecondary cellsComposite filmCarbon nanotube
The invention discloses a nickel sulfide / carbon nano tube flexible composite thin-film material and a preparation method and application thereof. The preparation method comprises the steps that urea and nickel nitrate are added into an aqueous solution of ethyl alcohol, then a silicon oxide / carbon nano tube thin film is added, soaked under normal temperature, taken out, placed in a dryer for to react for 10-15h under the temperature ranging from 100 DEG C to 110 DEG C, cooled naturally under room temperature, washed and dried, and then a silicate / carbon nano tube thin film is obtained for use;and deionized water and ethyl alcohol are mixed, Na<2>S is added to serve as a sulfur source, a solution with the concentration ranging from 1mg / mL to 2.5mg / mL is prepared, the silicate / carbon nano tube thin film obtained in the step 1 is soaked in the solution, subjected to a reaction for 10-15h under the condition of 150-200 DEG C, cooled naturally, washed and dried, and then the nickel sulfide / carbon nano tube flexible composite thin-film material is obtained. The nickel sulfide / carbon nano tube flexible composite thin-film material can be directly used as a sodium-ion battery cathode material.
Owner:TIANJIN UNIV
Nitryl asymmetric alpha-diimine nickel complex for preparation of ultrahigh-molecular-weight polyethylene and preparation method and application of nitryl asymmetric alpha-diimine nickel complex
ActiveCN108794544AHigh catalytic activityLow costNickel organic compoundsBulk chemical productionChemistryCoordination complex
The invention provides a nitryl asymmetric alpha-diimine nickel complex for preparation of ultrahigh-molecular-weight polyethylene and an intermediate, a preparation method and application of the nitryl asymmetric alpha-diimine nickel complex. The nickel complex has a single catalytic active center, regulation of polymer molecular weight and branching degrees can be realized through changing of aligand structure and polymerization conditions, and high catalytic activity, low cost, performance stability and the like are achieved. The preparation method is mild in condition, short in period andsimple in operation condition. The nickel complex can be applied to catalytic vinyl polymerization, the catalytic activity is up to 4.61*10<6>g mol<-1>(Ni) h<-1>, the weight-average molecular weightMw of prepared polyethylene fluctuates in a range of 8.2-32.8*10<5>g mol<-1>, molecular weight distribution is in a range of 1.8-2.8, the remarkable performance of polyethylene molecular weight control is achieved, and the nickel complex can be used for preparation of ultrahigh-molecular-weight polyethylene; obtained polyethylene is high in branching degree, the melt temperature Tm is in a range of 76.9-117.2 DEG C, and accordingly the polyethylene can be used as engineering plastic at a high environment temperature, and a promising industrial application potential is achieved.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Method for preparing acetic acid through carbonylation of methanol
ActiveCN106518657AImprove stabilityHigh selectivityMolecular sieve catalystsOrganic-compounds/hydrides/coordination-complexes catalystsIridiumProtein carbonyl
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Application of nickel sulfide/carbon nanotube flexible composite film material in negative electrode of lithium ion battery
The invention discloses the application of a nickel sulfide / carbon nanotube flexible composite film material in the electrode of a lithium ion battery. The preparation method of the composite film material comprises the steps that urea and nickel nitrate are added to an aqueous solution of ethanol, and then a silicon oxide / carbon nanotube film is added, immersed and removed at room temperature, placed in an oven at 100 DEG C to 110 DEG C for 10 to 15h, cooled naturally at room temperature, washed and dried to acquire a silicate / carbon nanotube film for standby; deionized water is mixed with ethanol, and Na2S is added as a sulfur source to prepare a solution with a concentration of 1 to 2.5mg / mL; the silicate / carbon nanotube film is immersed in the solution, reacts at 150 to 200 DEG C for 10 to 15h; and natural cooling, washing and drying are carried out to acquire the nickel sulfide / carbon nanotube flexible composite film material. The nickel sulfide / carbon nanotube flexible compositefilm material can be directly used as the negative electrode material of the lithium ion battery.
Owner:TIANJIN UNIV
4,4'-difluorobenzhydryl containing asymmetric alpha-diimine nickel complex, and intermediate, preparation method and application thereof
InactiveCN106632506AHigh catalytic activityLow costOrganic-compounds/hydrides/coordination-complexes catalystsNickel organic compoundsDiimineOxygen
The invention provides a 4,4'-difluorobenzhydryl containing asymmetric alpha-diimine nickel complex, and an intermediate, a preparation method and an application thereof. The nickel complex prepared by using the method provided by the invention has a single catalytic active center, can realize control of polymer molecular weight and branching degree by changing a ligand structure and polymerization conditions, and has the advantages of high catalytic activity, low cost, stable performance, etc. The preparation method has the advantages of mild conditions, short cycle and simple operation conditions. The nickel complex prepared by using the method provided by the invention can be used in a catalyst used for ethylene polymerization, and has the activity of catalyzing ethylene polymerization up to 2.20 * 10<7> g.mol<-1>(Ni).h<-1> under the condition of no oxygen; and the prepared polyethylene has a weight-average molecular weight Mw fluctuating in a range of (1.35-10.50) * 10<5> g.mol<-1> and molecular weight distribution in a range of 1.9 to 2.5. The nickel complex provided by the invention has good industrial application prospects.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Bacillus subtilis and application thereof in preparation of niacin by biocatalysis
InactiveCN101709283AStable catalytic performanceSimple processBacteriaMicroorganism based processesNiacinChemistry
The invention belongs to the field of biochemical engineering and discloses bacillus subtilis and application thereof in preparation of niacin by biocatalysis. The niacin is prepared by reacting 3-cyanopyridine serving as a substrate with bacillus subtilis KR2 (CGMCC NO.3242) serving as a biocatalyst at 5 to 35 DEG C and 100 to 300rpm for 15 to 96h, wherein the final yield of the niacin is over 90 percent and the purity of the obtained niacin is over 98 percent. The method of the invention is simple in operation and mild in reaction condition, and is suitable for application in scale industrial production.
Owner:NANJING HUAZHOU PHARMA
Pd-based alloy packaged molecular sieve catalyst, as well as preparation method and application thereof
ActiveCN112570015AAvoid flying temperatureHydrogenation reaction process safetyMolecular sieve catalystsHydrocarbon by hydrogenationChemistryMolecular sieve
The invention belongs to the technical field of molecular sieve catalysts, and discloses a Pd-based alloy packaged molecular sieve catalyst, a preparation method and applications thereof. The molecular sieve catalyst comprises a sodalite molecular sieve, and PdM nano-alloy particles are packaged in the sodalite molecular sieve; the preparation method comprises the following steps: firstly preparing a sodalite molecular sieve, then impregnating and loading Pd metal and an M metal precursor, roasting and reducing to form a molecular sieve catalyst impregnated and loaded with nano alloy particles; then uniformly mixing with a silica-alumina sol solution, uniformly stirring and mixing the solution, evaporating the solution to dryness, then adding ethylene glycol, grinding the mixture into uniform paste, carrying out hydrothermal reaction, and cooling, washing, drying, roasting and reducing the reaction product to obtain the catalyst. The molecular sieve catalyst is applied to acetylene selective hydrogenation reaction, can avoid an excessive hydrogenation process, and has relatively high acetylene conversion rate and ethylene selectivity.
Owner:TIANJIN UNIV
Asymmetric diimine pyridine complex with nitro-enhanced thermal stability and catalytic activity as well as preparation method and application of asymmetric diimine pyridine complex
ActiveCN109988100AImprove performanceMolecular weight regulationIron group organic compounds without C-metal linkagesCobalt organic compoundsDiimineEngineering plastic
The invention provides a diphenylmethyl-containing asymmetric diimine pyridine complex with nitro-enhanced thermal stability and catalytic activity, and a preparation method and application thereof. The complex has a single catalytic activity center, the molecular weight of a polymer can be regulated and controlled by changing a ligand structure and polymerization conditions, and the complex has the advantages of high catalytic activity, low cost, stable performance and the like. The complex and intermediates thereof have the advantages of mild preparation conditions, short period, simple operation conditions and the like. The asymmetric complex can be applied to a catalyst for ethylene polymerization and has high catalytic activity, and high molecular weight (up to 599.5 kg / mol) linear polyethylene is obtained; especially, the provided iron complex is high in thermal stability, and even at a high temperature of 100 DEG C, the catalytic activity can still be kept at 3.86* 10<6> g*mol<-1>(Fe)*h<-1>. Compared with reported non-nitro substituents (CH3, Cl), the complex has the highest activity and molecular weight. Therefore, the complex can be used at a high environmental temperatureas engineering plastic, and has great industrial application potential.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Cholesterol oxidase affinity matrix, synthesis and large-scale purification method thereof for cholesterol oxidase
ActiveCN102380347AEfficient purificationImprove purification efficiencyOther chemical processesOxidoreductasesFlavin adenine dinucleotideBenzene
The invention relates to a cholesterol oxidase affinity matrix formed by linking a cholesterol oxidase affinity ligand and a chromatography media; more preferably, the cholesterol oxidase affinity ligand is FAD (Flavin Adenine Dinucleotide) segmental riboflavin and the chromatography media is Sepharose, chitosan or polyvinyl benzene; the invention further provides a synthesis method for cholesterol oxidase affinity matrix, and a large-scale purification method for cholesterol oxidase by using the cholesterol oxidase affinity matrix; the cholesterol oxidase can be rapidly separated and purified in large scale by using the cholesterol oxidase affinity matrix provided by the invention; on the condition of only using one step of affinity chromatography, the pure cholesterol oxidase is obtained; the consumption time of the entire purification step is short, the active recovery rate of protein is higher and the large-scale popularization and application is applicable.
Owner:宁夏妙朗生物科技有限公司
Fluorine-containing alpha-diimine nickel coordination compounds for preparing polyolefin elastomer, intermediate, preparation method and application thereof
ActiveCN111303215AHigh catalytic activityMolecular weight regulationOrganic compound preparationNickel organic compoundsElastomerPolymer science
The invention discloses a class of fluorine-containing alpha-diimine nickel coordination compounds for preparing a polyolefin elastomer, and an intermediate thereof, wherein the coordination compoundhas a single catalytic activity center, the molecular weight of a polymer can be regulated and controlled by changing a ligand structure and polymerization conditions, and the coordination compound has the advantages of high catalytic activity, low cost, stable performance and the like. The invention further provides preparation methods of an asymmetric alpha-diimine nickel coordination compound containing a difluoro group, and an intermediate thereof, wherein the two preparation methods have the advantages of mild reaction conditions, short period, simple operation conditions and the like. The invention also provides uses of a metal nickel coordination compound and a catalyst system thereof, wherein the metal nickel coordination compound is used for catalyzing ethylene polymerization, shows good catalytic activity and obtains a polyethylene elastomer material with narrow molecular weight distribution. The obtained polyethylene elastomer material is high in branching degree, has good tensile strength, elongation at break and elastic resilience, is a thermoplastic elastomer material and has great industrial application potential.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Copper-zinc-tin-sulfur-selenium semitransparent solar cell device and preparation method thereof
InactiveCN113078225AImprove efficiencyGreat potential for industrial applicationsFinal product manufactureSemiconductor/solid-state device manufacturingElectrical batteryMaterials science
The invention discloses a copper-zinc-tin-sulfur-selenium semitransparent solar cell device and a preparation method thereof. The preparation method comprises the following steps: taking semitransparent fluorine-doped tin oxide (FTO) or FTO-MoO3 as a back electrode; the copper-zinc-tin-sulfur-selenium semitransparent solar cell device being composed of soda-lime glass, an FTO / FTO-MoO3 back electrode, a copper-zinc-tin-sulfur-selenium absorption layer, a cadmium sulfide buffer layer, a high-resistance intrinsic zinc oxide window layer, a low-resistance indium tin oxide window layer and a nickel-aluminum or silver electrode. The preparation method comprises the following steps of: cleaning commercial FTO conductive glass, evaporating MoO3 with different thicknesses on the FTO conductive glass, and then preparing each layer of film in sequence to obtain the CZTSSe semitransparent solar cell device taking FTO / FTO-MoO3 as the back electrode. The use of the semitransparent back electrode increases many possibilities for the application of the thin-film solar cell, including the generation of electric energy from the back, so that the thin-film solar cell becomes a double-sided device and has great industrial application potential.
Owner:NANJING UNIV OF POSTS & TELECOMM
Cattle-sourced hyaluronidase affinity medium and adsorption method thereof
ActiveCN108355618AHigh in proteinHigh recovery rateOther chemical processesSolid sorbent liquid separationSynthesis methodsHyaluronidase
The invention provides a cattle-sourced hyaluronidase affinity medium, formed by connecting affinity ligand and a chromatography medium. Preferably, the affinity ligand is a modified molecule of 6-chloromethyluracil and 2-aminobenzimi-dazole, and the chromatography medium is sepharose. The invention also provides a synthesis method of the cattle-sourced hyaluronidase affinity matrix, and a methodof using the affinity matrix to adsorb cattle-sourced hyaluronidase. By using the affinity matrix, the cattle-sourced hyaluronidase affinity can be specifically adsorbed and purified, and the application and popularization potential is great.
Owner:JIANGNAN UNIV
Stirring dispersing process for inoculating and refining aluminum alloy through amorphous alloy
The invention discloses a stirring dispersing process for inoculating and refining aluminum alloy through amorphous alloy and relates to the field of aluminum alloy processing. The stirring dispersing process comprises the four steps of preparation of the amorphous alloy, preparation of an amorphous ribbon, preparation of non-incubated aluminum alloy and inoculation of the aluminum alloy. With the adoption of the technical scheme, the steps are reasonable, the refining efficiency is high, obdurability of the aluminum alloy is improved significantly, inoculation is efficient, cost is low, and the stirring dispersing process has great industrial application potential and value.
Owner:JILIN UNIV
Gordona terrae C-6 and its desulfurization effect
InactiveCN100445361CGreat potential for industrial applicationsImprove featuresBacteriaFermentationBacterial strainGordona terrae
The invention relates to a Gordona terrae C-6 and its desulfurization effect, wherein the name of the bacterium is Gordona terrae C-6, it has been preserved in China General Microbiological Culture Collection Center (CGMCC), the collection number being CGMCC NO.1361. The bacterial strain is obtained through isolating from oil-water polluted soil around high-sulfur oil wells, and can be serve as catalyst for removing sulfur atoms in benzothiophene types sulfur-containing organic compounds, especially suitable for the removal of benzothiophenes organic heterocyclic sulfur in fossil fuels such as coal, oil and related products.
Owner:NANKAI UNIV
Method and device for circularly purifying sulfur dioxide and nitrogen oxide in flue gas
PendingCN113797733ANo secondary pollutionGood governanceGas treatmentDispersed particle separationElectrolysisFlue gas
The invention relates to a method and device for circularly purifying sulfur dioxide and nitrogen oxide in flue gas. The method comprises the steps of taking ammonium sulfate as a flue gas absorption medium to react in an electrolysis reactor to prepare an ammonium persulfate solution, and activating the ammonium persulfate solution entering an absorption tower spraying system through high-temperature flue gas to oxidize and absorb sulfur dioxide and nitrogen oxide in the flue gas. Meanwhile, ammonium sulfate subjected to reaction reduction can be extracted again and enters the electrolysis reactor, so that the purposes of circulating the oxidizing agent and efficiently removing sulfur dioxide and nitrogen oxide in the solution are achieved. The device is simple, small in occupied area, mild in condition and low in operation cost; secondary pollution is not generated in desulfurization and denitrification, and the device is environment-friendly and has wide pH adaptability; and N and S elements in the flue gas are recycled, and great industrial application potential is achieved.
Owner:KUNMING UNIV OF SCI & TECH
Functionalized hollow mesoporous silica nanospheres and its preparation method and application in adsorption of heavy metal ions
ActiveCN106861631BLarge specific surface areaStrong surface adsorptionOther chemical processesWater contaminantsSodium chloroacetateSide chain
The invention provides a functionalized hollow mesoporous silicon dioxide nanometer sphere, a preparation method thereof and an application in absorbing heavy metal ions. The hollow mesoporous silica nanospheres are used as raw materials, and then aminated with a silane coupling agent, and then the aminated nano-silica is combined with sodium chloroacetate to make the nano-silica grafted with the ability to adsorb metal ions bidentate functional groups. The inorganic-organic hybrid material has both the large specific surface area of the hollow mesoporous nano-silica microspheres, the hollow cavity and the abundant adsorption sites in the organic side chains. Effective separation of heavy metal ions such as Cu 2+ 、Cd 2+ 、Ni 2+ , Pb 2+ Wait. The raw material of the functionalized hollow mesoporous silica prepared by the invention is cheap and easy to obtain, the process is simple, the synthesis cost is low, and the invention has great application potential in the field of treating heavy metal ion pollution in water.
Owner:SUZHOU UNIV
Recombinant plasmid for producing pipecolinic acid, genetic engineering strain and method
ActiveCN107119065AIncrease growth rateIncrease productionBacteriaHydrolasesGenetic engineeringL-lysine-alpha oxidase
The invention discloses a recombinant plasmid for producing L-pipecolinic acid, a high-yield genetic engineering strain capable of enhancing heterogonous synthesis metabolic pathways of L-pipecolinic acid, and a production method of pipecolinic acid. The invention further discloses a preparation method of the recombinant plasmid and the genetic engineering strain. The strategy of constructing the high-yield genetic engineering strain comprises the following steps: adopting pTrc99a single-plasmid multi-gene tandem expression, linking L-lysine-alpha-oxidase genes (LysoX), glucose dehydrogenase genes (gcd), Pip2C reductase genes (DpkA) and lysine transportase genes (lysQ), optimizing a regulatory element for controlling transcription and translation in front of each gene, transforming the constructed plasmid pLMAIP-05 into competent cells of BL21 (DE3) of essential genes (cadA) in a knockout lysine degradation pathway, thereby obtaining the high-yield genetic engineering strain (MLPR08) capable of enhancing heterogonous synthesis metabolic pathways of L-pipecolinic acid. Due to expression of the lysine transportase, the rate of transporting a substrate L-lysine into the cells is improved, the fermentation time is shortened, and synthesis of the L-pipecolinic acid is further promoted.
Owner:CHONGQING UNIV
Squaramide derivative covalent triazine skeleton polymer and application thereof in catalyzing coupling of carbon dioxide and epoxide to prepare cyclic carbonate
ActiveCN114437363ALarge specific surface areaHigh nitrogen contentOrganic chemistryOrganic-compounds/hydrides/coordination-complexes catalystsPolymer sciencePtru catalyst
The invention relates to a squaramide-derived covalent triazine skeleton polymer and application thereof in catalyzing coupling of carbon dioxide and epoxide to prepare cyclic carbonate. Squaramide monomers are used as raw materials, a series of squaramide-derived covalent triazine skeleton polymers are synthesized through a high-temperature ionothermal method, and the squaramide-derived covalent triazine skeleton polymers are used as catalysts to prepare cyclic carbonate. According to the method, carbon dioxide and epoxide with different substituent groups are used as raw materials, corresponding cyclic carbonate is synthesized under the conditions that the reaction pressure is 0.1-2.5 MPa, the reaction temperature is 70-110 DEG C and the reaction time is 2-6 hours, and high-selectivity catalysis of synthesis of cyclic carbonate under mild, metal-free and solvent-free conditions is realized. The polymer shows excellent adsorption and conversion dual functions on carbon dioxide, the catalytic activity is not obviously reduced after five catalytic cycles, the problem that a homogeneous catalyst is not easy to separate is solved, and the polymer has a good application prospect.
Owner:QINGDAO UNIV OF SCI & TECH
n-5,6,7-hydroquinoline-8-arylimine nickel complex catalyst and its preparation method and application
ActiveCN105646597BGreat potential for industrial applicationsIncrease the degree of branchingNickel organic compoundsArylQuinoline
The present invention relates to a cycloalkyl-substituted N-5,6,7-hydro-quinoline-8-aryl imine nickel complex catalyst and a preparation method and application thereof. The N-5,6,7-hydro-quinoline-8-aryl imine nickel complex catalyst has a structural general formula as shown in a formula I. The catalyst complex can well catalyze vinyl polymerization under the effect of cocatalyst methylaluminoxane, diethylaluminium chloride and the like to obtain a low molecular weight high branching degree polymer, the catalyst complex also has high activity, the highest activity is up to 5.33*106g (PE) mol (Ni) <-1>h<-1>; the molecular weight of obtained polyethylene wax is 1430-3330g / mol, the molecular weight distribution is between 1.38-1.92, the branching degree is up to 422 / 1000C, and the catalyst complex has good prospects for industrial application.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Metal surface nano-composite processing device with stirring head capable of synchronously feeding powder, and method
InactiveCN102560472BIntegrity guaranteedUniform compositionMetallic material coating processesRotational axisWhole body
Owner:HOHAI UNIV
Laser and arc compound welding method
InactiveCN109967880AImproved Surface FormingImproved arc stabilityLaser beam welding apparatusArc stabilityMolten bath
The invention discloses a laser and arc compound welding method which comprises the following steps: (1) activating pulse laser and controlling a synchronous pulse signal; (2) activating an alternating current arc, and carrying out pulse laser-alternating current arc compound welding; and (3) in a welding process, adjusting the arc current to 40-500 A, the laser pulse peak value power to 1-6 KW, the basic value power to 200-1000 W, the laser pulse frequency to 10-100 Hz, the distance Dla between a laser impact point to the center of an arc molten bath to 1-6 mm and the distance from the tip ofan electrode to a workpiece to 1-5 mm till welding is finished. By coordination control between the pulse laser and alternating current arc negative half-wave regions, the arc stability can be improved, the surface forming of a welding region can be improved, and depth of fusion is increased by 1-2 times; by enhancing discharge in the alternating current arc negative half wave region by pulse laser, influence of processing parameters and environmental factors to welding stability can be reduced while the arc stability in a high speed condition is improved.
Owner:苏州欧米宁自动化科技有限公司
Method for constructing super-hydrophobic PVDF nanofiber membrane based on interface self-polymerization and application of super-hydrophobic PVDF nanofiber membrane
PendingCN113842791AImprove hydrophobicityChemically stableMembranesFatty/oily/floating substances removal devicesPolymer scienceSpinning
The invention provides a method for constructing a super-hydrophobic PVDF nanofiber membrane based on interface self-polymerization and application of the super-hydrophobic PVDF nanofiber membrane, and particularly relates to the technical field of super-hydrophobic materials. The preparation method comprises the following steps: dissolving PVDF in N, N-dimethylformamide to prepare a membrane casting solution, preparing a PVDF nanofiber base membrane by using an electrostatic spinning process, dissolving OTS containing active groups in an organic solvent, sequentially adding nanoparticles, PDMS and a cross-linking agent to prepare an oil-phase reaction solution, soaking the base membrane in ethanol, cleaning with deionized water, drying water drops on the surface of the base membrane by blowing, pouring the oil-phase reaction solution into the surface of the base membrane, introducing a high-hydrophobicity silane polymer into PDMS in situ by utilizing a hydrolysis self-polymerization reaction of OTS on a water-oil interface, pouring away the oil-phase reaction solution, and cleaning and drying a membrane body to obtain the super-hydrophobic PVDF nanofiber membrane. The method is simple in preparation process, does not need a modification device, is excellent in hydrophobicity and stable in property, is suitable for the fields of oil-water separation, self-cleaning coatings, membrane distillation and the like, and has a good industrial application prospect.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)
A kind of method utilizing Acinetobacter baumannii to synthesize flocculant
ActiveCN105039415BLow costGood flocculation effectMicroorganism based processesFermentationAdditive ingredientFreeze-drying
The invention discloses a method for synthesizing a flocculant by utilizing Acinetobacter baumanii. The method comprises the following steps: S1, strain activization: inoculating Acinetobacter baumanii ( / i) YNWH 226 in a solid medium for activization; S2, cultivation: inoculating a single colony obtained through activization into Congo red simulation waste water for cultivation, so as to obtain a bacterium liquid; S3, centrifugation: conducting centrifugation on the bacterium liquid obtained in the S2, and taking the supernatant after centrifugation to be mixed with ethyl alcohol or poly ethylene glycol; S4, conducting freeze drying on the precipitation product obtained in the S3, so as to obtain the flocculant. The flocculant, of which the main ingredient is polysaccharides, is synthesized through cultivation of Acinetobacter baumanii ( / i) YNWH 226 in Congo red simulation waste water. The method has the advantages that the flocculant preparation cost is relatively low; the efficient flocculation pH range is relatively wide; the flocculation effect is relatively good; the industrial application potentiality is high.
Owner:韶关鹏瑞环保科技有限公司
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