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Shewanella spp and application thereof in microbial fuel cell

A Shewanella and fuel cell technology, applied in the biological field, can solve problems such as reports of unseen systems, and achieve the effects of broad industrial application potential, high electrical activity, and high electrical activity.

Inactive Publication Date: 2010-09-22
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, there have been no systematic reports on the use of marine microorganisms to generate electricity.

Method used

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  • Shewanella spp and application thereof in microbial fuel cell
  • Shewanella spp and application thereof in microbial fuel cell
  • Shewanella spp and application thereof in microbial fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Screening of S4 bacterial strain

[0042] Marine sediment samples were collected from the bay near Xiamen, China. Take 2 grams of sediment samples and suspend them in 250 milliliters of microbial fuel cell anolyte solution for enrichment culture. The composition of the anolyte medium (RM) is: 1000 ml deionized water, 19.0g NaCl, 4.9g MgCl 2 , 1.30 g K 2 HPO 4 , 0.45g KH 2 PO 4 , 0.19g (NH 4 ) 2 SO 4 , 0.25gMgSO 4 , 10ml Wolfe’s Minerals, 0.05g CaCl 2 , 0.02g L-arginine, 0.02g glutamic acid, 0.02g serine and 0.10g yeast powder, wherein 10mmol / L of sodium lactate (DL-lactate) was added as an electron donor. The composition of the battery catholyte is the same as that of the anolyte except that amino acids and yeast powder are not added, and the corresponding sodium chloride needs to be added to the catholyte to balance the osmotic pressure between the catholyte and the anolyte. The microbial fuel cell device adopts H-shaped structure ( figure 1 )...

Embodiment 2

[0043] Example 2: Acquisition and induction of S4 electrogenic seeded cells

[0044] Pick the S4 strain colony cultured on the LB plate, inoculate it into 20 ml of RM medium, add 20mmol / L sodium lactate as electron donor and carbon source, cultivate at 30°C for 12 hours at 180 rpm, and then press 0.1% The inoculum amount was added to 100 ml of anaerobic RM medium, in which 20 mmol / L sodium lactate and 20 mmol / L sodium fumarate were added as electron donor and electron acceptor, respectively, and cultured at 30°C for 12 hours, then Inoculate 0.1% into anaerobic RM medium with 50mmol / L ferric citrate and 20mmol / L sodium lactate as electron acceptor and donor, 30? After culturing for 12 hours, the bacterial cells were obtained by centrifugation under anaerobic environment. Place the cells in 100 ml of anaerobic RM medium, mix and shake for 1 to 2 hours, and centrifuge to obtain the cells again.

Embodiment 3

[0045] Example 3: Electricity production using resting cells of the S4 strain

[0046] According to the cultivation and inoculation method described in Example 3, the cells in the late logarithmic period of growth were obtained from 1000ml of ferric citrate and sodium lactate as the anaerobic RM medium of the electron acceptor and donor, and were centrifuged under anaerobic conditions. 100ml of anaerobic phosphate buffer (50mM sodium phosphate buffer, pH=7.0) was resuspended and centrifuged to wash the cells twice, and finally resuspended in 5ml of anaerobic phosphate buffer, which was used as an inoculum and inoculated to anode in a microbial fuel cell. Both the anolyte and catholyte of the microbial fuel cell use 50mM sodium phosphate buffer solution (pH=7.0), the anode is fed with high-purity nitrogen to ensure an anaerobic environment, and the cathode is fed with air to make oxygen as the final electron acceptor. See Example 1 for other settings and conditions of battery ...

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Abstract

The invention discloses shewanella spp and application thereof in a microbial fuel cell and relates to the technical field of biology. The shewanella spp is shewanella xiamenensis sp.nov.S4 preserved in the 'China Center for Type Culture Collection' in January, 2009 with the preservation number of CCTCC M 209017. The shewanella spp has the characteristics of negative Gram stain, rod-shaped and straight thallus, two circular ends, diameter of 0.3 to 1.2mu m, length of 2.5 to 5.8mu m, single or paired existence and short-chain arrangement, single flagella on the thallus, mobility, capability of producing electricity in a seawater environment and higher electricity production activity and is obtained by separating marine sediments near the sea.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a new Shewanella bacterium, namely Shewanella xiamenensis S4, and its application in microbial fuel cells. Background technique [0002] my country's energy shortage situation is severe, and the level of energy resources per capita is far lower than the world average. According to the statistics of British Petroleum Corporation in 2006, the remaining recoverable reserves of oil and natural gas in my country are only 7.69% and 7.05% of the world average. In addition, carbon and sulfur in petroleum products are released into the atmosphere in the form of carbon dioxide and sulfur dioxide after combustion, causing serious pollution to the environment and seriously threatening the global ecological balance. As these issues of energy shortage, waste disposal, and global warming are getting more and more attention, there is an urgent need to develop renewable new energy sources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16H01M4/90C12R1/01
CPCY02E60/527Y02E60/50Y02P70/50
Inventor 章晓波黄杰勋
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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