The invention provides a high-temperature-resistant radiation-proof coating, which comprises the following raw materials in parts by weight: 10-15 parts of zirconium sol, 6-10 parts of aluminum dihydrogen phosphate, 8-12 parts of expanded graphite, and 5-10 parts of carbon nanotubes , 1-5 parts of titanium dioxide, 5-10 parts of glass powder, 5-10 parts of talc powder, 20-25 parts of mica powder, 0.5-3 parts of hydroxypropyl methylcellulose, dicyclopentenyloxyethyl acrylate 1 to 5 parts and 20 to 30 parts of water. The present invention also provides a preparation method of the above-mentioned high temperature resistant radiation protection coating. The components in the above-mentioned high-temperature-resistant and radiation-resistant coating provided by the present invention cooperate with each other and complement each other, so that the coating has excellent anti-corrosion, radiation protection, antibacterial, flame-retardant, and high-temperature resistance under the premise of maintaining good adhesion. , anti-cracking, storage stability and other characteristics.