31results about How to "Change conformation" patented technology

The invention discloses a preparation method of high tenacity silk fibroin gel. Ordinary silk fibroin solution undergoes crystallization-inducing treatment to obtain crystalline silk fibroin solution, then mixes it with ordinary silk fibroin solution, and finally obtains high-tenacity silk fibroin gel under the action of self-assembly. This technology can control the gelation time, toughness and other properties of hydrogel by changing the concentration of silk fibroin solution, the size and content of crystalline silk fibroin, self-assembly temperature and other parameters. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, and there is no waste residue, which provides a new method for the preparation of functional hydrogels and can be used in biomedicine and tissue engineering and other fields.

The invention discloses a self-assembled collagen stimulating microsphere with a natural shell-core structure and a preparation method thereof. Utilize the electrostatic interaction between polyamino polycationic natural polymer and sodium hyaluronate (HA) to change the conformation of HA molecules, reduce the viscosity of high molecular weight HA neutral solution, and promote the formation of micron-sized HA microspheres in the emulsification system . Utilize the electrostatic interaction between polyamino polycationic natural polymer and HA and the condensation and cross-linking reaction of acid amine to promote the precipitation of microspheres. The method skillfully solves the problem of high viscosity of high molecular weight HA neutral solution, and has simple steps and environmentally friendly technical route. The prepared collagen-stimulated microspheres have a shell-core self-assembly structure, and the components are all natural polymers, which can be rapidly degraded by specific biological enzymes when side reactions occur, ensuring the safety of product injections; at the same time, the microspheres have good Excellent mechanical strength and long degradation time can effectively stimulate collagen secretion for a long time, and have a good application prospect in the field of soft tissue filling, especially medical cosmetic filling.

A viscosity-reducing high-efficiency water-reducing polycarboxylate water-reducer and its preparation method, the water-reducer is composed of macromonomers such as isopentenol polyoxyethylene ether monomers, unsaturated hydroxy acid monomers or derivatives thereof, Or the unsaturated acid with trans structure is prepared by free radical polymerization under the action of reducing agent, oxidizing agent and chain transfer agent by intermittent dropping. The anchor groups and side chains in the water reducer of the present invention are block-distributed molecular structures, which can give full play to the electrostatic repulsion and steric hindrance effects; in addition, the hydroxyl groups introduced by unsaturated hydroxyacid monomers or their derivative monomers Polar groups such as carboxyl and carboxyl groups increase the proportion of hydrophilic groups and reduce the surface tension of the cement pore solution; in addition, the trans structure represented by fumaric acid and dihydroxy fumaric acid derivatives, due to its special The structure, that is, the rigidity of the monomer itself and its two carboxyl groups are distributed on the opposite side of the side chain, which makes it easy to show a stretched structure in space, reduces the possibility of molecular chain entanglement, and significantly increases the polycarboxylate superplasticizer. Adsorption on the surface of cement, thereby improving the viscosity-reducing effect of polycarboxylate superplasticizer.

The invention discloses a multiple-effect polyclone antibody for animals, prepared according to the following method with steps of: A, adopting grouped poly immunogen polypeptide as immunogen, which at least contains the sequence of the immunogen polypeptides as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, or a sequence with immunogenicity derived from the sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 by substituting, deleting, and / or adding one or more amino acid; B, immunizing animals: immunizing a target animal with the immunogen polypeptide of the step A and obtaining antiserum; and C, obtaining polyclone antibody: separating and purifying the antiserum immunoglobulin obtained in the step B to obtain the polyclone antibody. The polyclone antibody disclosed by the invention has extensive application in preparing integrate disease control drugs and immunopotentiating drugs for livestock.

The invention relates to an absorbable orthopedic apparatus material having antibiosis and bone growth promotion functions, and a preparation method thereof. The orthopedic apparatus material is obtained by compounding medical pure magnesium or magnesium alloy with an absorbable high-molecular polymer, the surface of the pure magnesium or magnesium alloy has a porous ceramic layer, the surface of the porous ceramic layer is a functional load layer loaded with bone morphogenetic proteins and copper ions with B-type gelatin as a carrier by adopting an electrophoresis-electrodeposition process, the volume fraction of the pure magnesium or magnesium alloy is 1-99%, the micropore aperture and the thickness of the porous ceramic layer on the surface of the pure magnesium or magnesium alloy are 1-100[mu]m and 1-100[mu]m respectively, and the surface of the porous ceramic layer is the functional load layer loaded with bone morphogenetic proteins and copper ions with B-type gelatin as the carrier by adopting the electrophoresis-electrodeposition process. An orthopedic apparatus can slowly release copper ions having a sterilizing effect and bone morphogenetic proteins having a bone growth promotion effect in the use process, can be applied to restore and fix various bone injuries, and has a wide application prospect.

The invention discloses a preparation method of silk fibroin thixotropic gel. Firstly, common silk fibroin solution and crystallized silk fibroin particle solution are prepared, then the crystallized silk fibroin particle solution is mixed with common silk fibroin solution of specific concentration, and left standing to obtain silk fibroin thixotropic gel. This technology can control the gelation time and light transmittance of hydrogel by changing the concentration of silk fibroin solution, the concentration of crystallized silk fibroin particle solution, the mixing ratio of the two, and the treatment temperature. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, it is convenient for large-scale preparation, there is no waste residue, green and pollution-free, and it is easy for industrialization.

The invention discloses a method for realizing efficient co-production of malt syrup and rice protein by adopting rice processing by-products. Broken rice and rice bran are adopted as the raw materials. The method comprises the following processing steps: (1) carrying out mechanical pretreatment; (2) carrying out ultrasonic treatment; (3) carrying out mild biological activating; (4) carrying out degreasing pretreatment; (5) carrying out hydrolysis and liquefying by adopting alpha-amylase; (6) carrying out separating, namely, carrying out centrifugalizing on the liquefied liquid obtained in the step (3) at 3200r / min, thus obtaining the supernate and sediment; (7) preparing broken rice and rice brain protein; (8) carrying out modified treatment on saccharifying enzyme; and (9) preparing the broken rice and rice bran malt syrup. With the adoption of the method provided by the invention, the malt syrup and rice protein by-products with good quality can be prepared, and the utilization value of the rice processing by-products is favorably improved.

The invention belongs to the technical field of agricultural product processing and storage, and provides an invention that improves the foamability of egg white liquid and prolongs its shelf life. Prepare fresh egg white liquid, add compound protease in a certain proportion, and enzymatically hydrolyze after proper ultrasonic treatment. After the enzymatic hydrolysis is completed, the egg white liquid is deenzyme-killed and sterilized by ultrasound, and finally pasteurized properly by raising the temperature again. The foaming power of the obtained egg white liquid is increased by 3 times, the foam stability has no significant difference, and the storage period is extended by 4 times. This process effectively solves the problems of foamability and shelf life, meets the needs of enterprises, has high utilization rate of equipment, and is conducive to improving the benefits of egg white liquid enterprises.

The invention discloses a method for efficiently co-producing maltodextrin and rice protein through rice processing byproducts. The method is characterized in that broken rice and rice bran are used as the raw materials. The method comprises the following processing steps: (1) mechanically crushing; (2) ultrasonically processing; (3) lightly biologically activating; (4) debranching and preprocessing; (5) hydrolyzing and liquidating with alpha-amylase; (6) separating; (7) preparing broken rice and rice bran protein; (8) preparing broken rice and rice bran maltodextrin. With the adoption of the method, high-quality broken rice and rice bran maltodextrin and rice protein byproducts can be prepared, so that the utilization value of the rice processing byproducts can be further increased.

The invention discloses a blood collection tube with the function of protecting and stabilizing the precursor of gastrin releasing peptide in serum or plasma, comprising a test tube, a sealing rubber plug and a plastic outer cap are arranged at the opening of the test tube, and the test tube is prefilled with cheese Protectant formulated with protein, sodium lauryl sulfate and aprotinin. Because the protective agent is pre-filled in the blood collection tube, the storage time of the ProGRP sample is greatly extended (the sample can be stored stably for 7 days at 2-8°C). On the one hand, it is convenient for clinical operation and application. Retesting can be carried out, which reduces the probability of false negative test results and improves the accuracy and clinical application value of the test results of this item.

The invention provides a production method of photo-induced energy-storage fluorescent drawings. Photo-induced energy-storage noctilucent powder, natural pigment and an adhesive are used as raw materials to produce a base material, and spun silk, paper and cloth are used as substrates for drawing, wherein a method for preparing the base material comprises the following steps that 1, an anionic surfactant is dissolved in n-butyl alcohol to form an oil phase solution; 2, cow glue is added into water, and through stirring, a collagen solution is obtained; 3, the photo-induced energy-storage noctilucent powder and the natural pigment are added into the collagen solution in the step 2, and through stirring, a pigment stock solution is obtained; 4, the pigment stock solution obtained in the step3 is added into the oil phase solution, and through ultrasonic concussion, a base material stock solution is obtained; 5, the base material stock solution obtained in the step 4 is subjected to spraydrying to obtain the base material. According to the production method of the photo-induced energy-storage fluorescent drawings, the anionic surfactant is used for preparing the oil phase solution, the prepared pigment stock solution is added into the oil phase solution, through ultrasonic concussion, oil phase molecules are broken, and then the pigment stock solution is evenly dispersed in the oil phase molecules to improve the water resistance of the pigment stock solution.

The invention provides an artificial ligament which comprises an inner core and an outer layer from inside to outside in sequence. The inner core is made of ozonized polyethylene glycol terephthalate, and the outer layer is made of polysaccharide or polymer polymerized from hydrophilic group contained allyl monomer and is grafted on a free radical locus of the inner core. A preparation method of the artificial ligament includes: subjecting a ligament of a polyethylene glycol terephthalate material to reaction with ozone so as to obtain the polyethylene glycol terephthalate with a free radical; under protection of inert gas, subjecting a solution containing monomer molecules and the polyethylene glycol terephthalate containing the free radical to sealed polymerization reaction so as to obtain the artificial ligament. The artificial ligament is good in biocompatibility, and capable of restraining bacterial infection, changing protein conformation adhered on surface of a material, reducing inflammation and promoting adhering, growing and fibroblast of fibroblast after being planted in a human body. Collagen protein synthesis on the surface of the artificial ligament is improved, and infiltration of new ligament tissue is facilitated.