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Blood collection tubes for protection and stabilization of gastrin releasing peptide precursor in serum or plasma

A gastrin-releasing peptide and blood collection tube technology, which is applied in the field of medical testing, can solve the problems of false negative test results and inconvenient clinical application, reduce the probability of false negatives, improve accuracy and clinical application value, and prolong storage time Effect

Active Publication Date: 2021-01-26
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid degradation of ProGRP samples, on the one hand, will cause false negative test results, and on the other hand, it will not be possible to carry out follow-up experiments, such as re-testing when the test results of individual samples are abnormal, which brings great inconvenience to the clinical application of this project.

Method used

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  • Blood collection tubes for protection and stabilization of gastrin releasing peptide precursor in serum or plasma
  • Blood collection tubes for protection and stabilization of gastrin releasing peptide precursor in serum or plasma
  • Blood collection tubes for protection and stabilization of gastrin releasing peptide precursor in serum or plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 The preparation method of the blood collection tube of the present invention

[0022] The first step is to prepare the ingredients of protective agent 4 in proportion (calculated in 1L):

[0023] Measure 1000mL of purified water, add NaH successively 2 PO4·2H 2 O 0.59g, Na 2 HPO4·12H 2 O 5.8g, NaCl9g, casein 10g, sodium lauryl sulfate 20g, aprotinin 2g, ProClin300 1mL, stir until the mixed solution is clear and transparent. Then use a vacuum freeze dryer to perform freeze-drying treatment to obtain a freeze-dried cake, and then use a homogenizer to process the freeze-dried cake to obtain a uniform powder, which is the protective agent 4.

[0024] The second step is to clean the inner wall of the test tube 1, inject the prepared protective agent 4 into the bottom of the test tube 1 under the aseptic working environment of the ultra-clean bench, and the amount added is 1-5% (w / v) of the blood volume of the blood collection tube;

[0025] The third step i...

Embodiment 2

[0026] Example 2 Sample storage time comparison

[0027] Using the blood collection tube of the present invention and the conventional common tube blood collection tube to process blood samples at the same time, a total of 10 samples were assessed. After the blood was drawn, the samples were turned upside down 10 times, placed in a 37°C incubator for 30 minutes, and then centrifuged at 4000 rpm for 10 minutes after being taken out; after centrifugation, they were stored at 2-8°C. At the time points of 0h, 3h, 1 day, 3 days, 5 days, 7 days, and 14 days, the samples were tested using a commercially available gastrin releasing peptide precursor detection kit from a certain company, and the two blood collection tubes were assessed See Table 1 and Table 2 for the stability of the samples under storage conditions of 2-8°C.

[0028] Table 1 Storage time of blood collection tube samples of the present invention under the condition of 2~8°C

[0029]

[0030] Table 2 The storage ti...

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Abstract

The invention discloses a blood collection tube with the function of protecting and stabilizing the precursor of gastrin releasing peptide in serum or plasma, comprising a test tube, a sealing rubber plug and a plastic outer cap are arranged at the opening of the test tube, and the test tube is prefilled with cheese Protectant formulated with protein, sodium lauryl sulfate and aprotinin. Because the protective agent is pre-filled in the blood collection tube, the storage time of the ProGRP sample is greatly extended (the sample can be stored stably for 7 days at 2-8°C). On the one hand, it is convenient for clinical operation and application. Retesting can be carried out, which reduces the probability of false negative test results and improves the accuracy and clinical application value of the test results of this item.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a blood collection tube for protecting and stabilizing the gastrin releasing peptide precursor in serum or plasma. Background technique [0002] In recent years, lung cancer has become the number one killer of malignant tumors in my country. Lung cancer is divided into non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Among them, non-small cell lung cancer accounts for about 85%, and the 5-year survival rate is 15%. Although small cell lung cancer only accounts for about 15%, the 2-year survival rate is only 1%. 70% of lung cancer patients are locally advanced or metastatic at the time of diagnosis, losing the best opportunity for surgery. If lung cancer can be diagnosed at an early stage, the 5-year survival rate can increase to more than 60%. [0003] Compared with traditional imaging methods such as CT and MRI, tumor markers not only have the advantages of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574A61B5/154
CPCA61B5/15003A61B5/150343A61B5/150755A61B5/154G01N33/57423G01N33/57488
Inventor 渠文涛史小芹陈小玲项立红刘功成渠海冉盼盼付光宇吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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