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A kind of pleiotropic polyclonal antibody for animals, its preparation method and application

A polyclonal antibody, animal technology, applied in the direction of antibodies, drug combinations, anti-infective drugs, etc., to achieve a long shelf life, fill the immune gap, and avoid immune failure.

Active Publication Date: 2019-03-08
河北普德动物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A kind of pleiotropic polyclonal antibody for animals, its preparation method and application
  • A kind of pleiotropic polyclonal antibody for animals, its preparation method and application
  • A kind of pleiotropic polyclonal antibody for animals, its preparation method and application

Examples

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Embodiment 1

[0020] Example 1, Preparation and application of multi-effect polyclonal antibody for animals

[0021] A. Preparation of multiple group immunogens: grouped multiple immunogen polypeptides, which at least include the immunogen polypeptides shown in SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3; its preparation method It is as follows: firstly amplify the structural gene of the immunogenic polypeptide by PCR method, integrate the structural gene of the immunogenic polypeptide with the expression vector by restriction endonuclease to form a recombinant expression vector, transfer the recombinant expression vector into host cells to induce expression to obtain the immunogen Peptides; sequences can also be provided and commissioned by Shanghai Sangong, Novagen and other companies to prepare by bioengineering methods;

[0022] B. Animal immunization: immunize target animals with the immunogenic polypeptide of step A, and take antiserum; specifically include the following steps:

[0...

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PUM

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Abstract

The invention discloses a multiple-effect polyclone antibody for animals, prepared according to the following method with steps of: A, adopting grouped poly immunogen polypeptide as immunogen, which at least contains the sequence of the immunogen polypeptides as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, or a sequence with immunogenicity derived from the sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 by substituting, deleting, and / or adding one or more amino acid; B, immunizing animals: immunizing a target animal with the immunogen polypeptide of the step A and obtaining antiserum; and C, obtaining polyclone antibody: separating and purifying the antiserum immunoglobulin obtained in the step B to obtain the polyclone antibody. The polyclone antibody disclosed by the invention has extensive application in preparing integrate disease control drugs and immunopotentiating drugs for livestock.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a polyclonal antibody prepared by utilizing multiple group antigens and its application. Background technique [0002] The molecular structure on an antigen that can cause the body to produce antibodies is an antigenic determinant, and there can be several different antigenic determinants on an antigen that cause the body to produce different antibodies, and finally the antibodies produced are plasma cells. A population of plasma cells acting against only one antigenic determinant is an inbred, ie, clone. Monoclonal antibody refers to the antibody produced by a B lymphocyte that stimulates the body by an antigenic determinant. [0003] Corresponding to monoclonal antibodies are polyclonal antibodies, which stimulate the body by a variety of antigenic determinants, and correspondingly produce various monoclonal antibodies. These monoclonal antibodies are mixed together to form polycl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/06A61K39/395A61P37/04A61P31/00
CPCA61K2039/507C07K16/06
Inventor 刘明陆安王晔娜贺丙强贾永兵
Owner 河北普德动物药业有限公司
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