The invention belongs to the field of analytical chemistry, and particularly discloses indirect iodometry dynamics spectrophotometry for measuring vitamin C. The spectrophotometry specifically comprises steps as follows: at the room temperature, using a cuvette of 1 cm as a reaction container, adding 0.50-1.40 mL of a 200 mu g / mL vitamin C standard solution and 1.60-2.50 mL of a 0.5% starch solution to enable the volume of the mixed solution to be 3.00 mL, adding 0.20 mL of a 0.200 mg / mL potassium iodate solution and 0.10 mL of a 0.15 mol / L of sulfuric acid solution, evenly shaking the mixture, quickly placing the mixture into a reagent blank for a photometer, performing dynamics spectrophotometry scanning at the wavelength of 600 nm to measure the peak absorbance within a short time, wherein the peak absorbance has a good linear relation with the concentration of the vitamin C. The spectrophotometry has the advantages that the spectrophotometry is convenient, accurate, micro and quick, used drugs are low in cost, easy to obtain, non-toxic, pollution-free and environment-friendly and the like, and is used for measuring the vitamin C in biological samples, drug samples and the like.