Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer

Abstract

The invention relates to a method for measuring the total sugar content of a chlortetracycline fermentation broth with a refractometer, and the method is characterized by accurate measuring result and low cost. The method comprises the steps of: (1) collecting original data: measuring the sugar content data of a standard sample by iodimetry, with the sugar content of the sample at a gradient concentration of 0-17%; (2) inputting the data measured in step (1) into the refractometer; (3) dropping the sample with sugar content of 0-17% for measuring directly in a determination analysis pool so as to conduct measuring. The method of the invention has the advantages of accurate measuring result and fast detection speed. Meanwhile, after one time of standard data input, no input and detection through iodometry are needed once again during sugar content detection of the sample at a later stage, so that a lot of reagents can be saved. The method of iodometry is complicated for operation, while the adoption of a refractometer is simple in operation. The employment of a refractometer for detection reduces the labor intensity of technicists and improves the working efficiency.

Description

Technical field [0001] The invention relates to the field of chemical sample detection, in particular to a test method for measuring the total sugar content of chlortetracycline fermentation broth with a refractometer. Background technique [0002] The current method for detecting total sugar in feed chlortetracycline fermentation broth has been using the traditional iodometric method for many years, which not only requires a lot of work, but also takes a long time to determine, and cannot provide accurate and reliable analysis data for production in time. Moreover, the cost of the measurement is relatively high. The iodometric method is used to determine the total sugar of the chlortetracycline fermentation broth, and the measurement reagent is expensive. At the same time, the waste liquid used in the measurement will also pollute the environment, and sometimes in order to reduce the cost, it is often concluded that the measurement result is only one time. The measurement result...

AI Technical Summary

Problems solved by technology

[0002] The traditional iodometric method has been used for many years for the detection of total sugar in feed aureomycin fermented broth, which not only requires a lot of work, but also takes a long time to measure, and cannot provide accurate and reliable analysis data for production in time.

And the cost of determination is higher, adopt iodometric method to measure the total sugar of chlortetracycline fermentation broth, its determination reagent is expensive

Simultaneously, the waste liquid used in the determination will also pollute the environment, and sometimes in order to reduce the cost, it is often concluded that only one measurement is made, and the error rate of the measurement results is high, which cannot meet the analysis and measurement requirements. Low, direct detection by refractometer, the measurement results also contain deviations, which cannot meet the requirements of analysis and determination

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