Detection of beta-lactamase in milk and milk products by iodometry

A technology of lactamase and dairy products, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, etc. It can solve the problems of poor specificity and repeatability, no detection methods, and inability to Monitor and control the quality of raw milk from the source to achieve good social and economic benefits

Inactive Publication Date: 2011-05-04
BEIJING WUZI UNIVERSITY
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection techniques of β-lactamase in milk include cup and saucer method, iodometric method, acidity method, high performance liquid chromatography and enzyme-linked immunosorbent method, etc., but all of them have defects, such as poor specificity and repeatability, and large background interferen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection of beta-lactamase in milk and milk products by iodometry
  • Detection of beta-lactamase in milk and milk products by iodometry
  • Detection of beta-lactamase in milk and milk products by iodometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in conjunction with the examples, but not limited thereto.

[0025] Prepare the required solution:

[0026] a. pH 6.2 phosphate buffer solution: take 1.36g KH 2 PO 4 and 1.74g K 2 HPO 4 , were dissolved in 1000mL of distilled water, respectively, the two were taken 8mL and 2mL after mixing.

[0027] b. Penicillin solution: weigh 25 mg of ampicillin, add distilled water to dissolve to 25 mg / mL.

[0028] c. pH 4.7 acetate buffer solution: Take 11.4mL HAc and 16.4g NaAc, dissolve them in 1000mL water respectively, take 6.3mL and 3.7mL of the two respectively and mix them.

[0029] d. Iodine-starch developer: dissolve 1 gram of starch in 100 ml of distilled water to make a starch solution with a concentration of 1%; weigh 6.5 g of iodine and 17.5 g of potassium iodide, dissolve in 50 ml of water, dilute to 500 ml, and mix well , make a concentration of iodine standard solution of 0.1mol / L, dilute it 30 times and mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for rapidly detecting beta-lactamase in milk and milk products. The fundamental principle is that: the beta-lactamase can decompose penicillin, and a decomposition product penicilloic acid competes with starch for free iodine to damage a composite formed by the iodine and the starch to further change blue solution into colorless solution; and the penicillin and an iodine-starch color reagent are added into the treated milk and the treated milk products, blank control is performed, and qualitative and semi-quantitative analysis is performed on the beta-lactamase according to the change of the color of the solution. In the invention, the novel method for detecting the beta-lactamase in the milk and the milk products is established in terms of the concentration of the penicillin, a pH value of buffer solution, the concentration and proportion of the color reagent, actual sample purification conditions, reaction temperature, centrifugation conditions and the like. In the method, the lowest qualitative and semi-quantitative detection limit is 1.5 U/mL. The technology is popularized, and a detection kit is researched and developed to realize real-time online detection, thereby bringing good social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of rapid detection, in particular to detecting β-lactamase added in milk and milk products by using a chemical method, i.e. iodometric method. Background technique [0002] As the country pays attention to food safety issues and some dairy companies have put forward the goal of no milk resistance in 2010, the problem of antibiotic residues has become one of the important factors affecting the safety of dairy products. Currently, penicillin, as a β-lactam drug, is the drug of choice for the treatment of bovine mastitis and is the most common residual antibiotic in milk. Because most domestic dairy companies adopt the principle of reducing the price of milk with excessive antibiotic residues, driven by economic interests, some illegal milk stations artificially use some biological agents to degrade the antibiotics remaining in milk in order to seek their own economic interests. Artificial "antibiotic-free mil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78
Inventor 沈丽吴蕾张耀荔陈红丽芮嘉明刘艳
Owner BEIJING WUZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap