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173 results about "Semiquantitative analysis" patented technology
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A semi-quantitative analysis would be used to determine the number of tires produced every day since a manufacturing plant began production. A semi-quantitative analysis would be used by a meteorologist to ascertain the annual average temperature of a specific location.
Method and system for rapid biomolecular recognition of amino acids and protein sequencing
InactiveUS6846638B2Low costHigh sensitivityBioreactor/fermenter combinationsBiological substance pretreatmentsProtein Sequence DeterminationDigestion
Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and / or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and / or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.
Owner:NANOBIODYNAMICS
Analyte assays and devices
InactiveUS6235539B1Minimize any potential sample diminutionMinimizes potential for sample contamination and cross-contaminationBioreactor/fermenter combinationsBiological substance pretreatmentsAnalyteReagent
The present invention provides a method and a device that utilizes capillarity-mediated, chromatographic transport, for the qualitative or semi-quantitative analysis of selected analytes in liquid samples. The device utilizes an applicator / collection device for collecting and administering the sample to the flow path such that reagent(s) flow through the applicator / collection device, washing the sample into the reaction pathway. The device farther utilizes an air gap between the initial location of the reagent and the reaction pathway to funnel the reagent efficiently through the sample so as to collect all or substantially all of the sample and make it available for the reaction(s).
Owner:IDEXX LABORATORIES
Method and system for rapid biomolecular recognition of amino acids and protein sequencing
InactiveUS20050164264A1Quick analysisBioreactor/fermenter combinationsBiological substance pretreatmentsProtein Sequence DeterminationDigestion
Owner:NANOBIODYNAMICS
Electrodes selective for solid-contact ions based on carbon nanotubes
InactiveUS20100193376A1Weather/light/corrosion resistanceIndividual molecule manipulationAnalyteChemical species
The invention defines an all-solid-contact ISE which comprises a transducer layer of carbon nanotubes which brings the sensing layer and conducting element into contact. The invention also defines a method for the preparation of said all-solid-contact ISE and the use of the same for the qualitative, quantitative or semi-quantitative analysis of analytes. Said all-solid-contact ISE makes it possible to detect or quantify highly diverse chemical species in a reliable and reproducible manner, with the added advantages derived from its simplicity and low construction cost.
Owner:UNIV ROVIRA I VIRGILI
Nano-colloidal gold marker immunization measurement method for testing carbofuran pesticide
InactiveCN1445548AQuick checkChanged multi-step cumbersome detection problemBiological testingCarbofuranPesticide residue
A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.
Owner:SHANGHAI JIAO TONG UNIV
Systems and methods for Intra-operative semi-quantitative threshold neural response testing related applications
Owner:CHECKPOINT SURGICAL
Method for realizing semi-quantitative analysis to trace mixture by use of surface enhanced raman spectroscopy
ActiveCN104914089AGood for observing cluster analysisImprove accuracyRaman scatteringSurface-enhanced Raman spectroscopyPrincipal component analysis
The invention discloses a method for realizing semi-quantitative analysis to trace mixture by use of the surface enhanced raman spectroscopy, and in particular relates to a qualitative identification method for carrying out semiquantitative analysis on a trace mixture by determinng Raman spectrum of a trace chemical substance based on surface enhanced Raman scattering effect and improving main component analysis. The method discosed by the invention adopts a lever law and a triangle parapllel line reading method to realize the semiquantitative analysis of the mixture components. The method can be used for analyzing mixture of entirely different chemical substances and can be used for analyzing mixture of homologous series. The method can realize semi-quantitative analysis to components of mixture based on the PCA (Principal Component Analysis) qualitative analysis and is a simple, rapid and accurate mixture identification method.
Owner:广西三环高科拉曼芯片技术有限公司
Semi-quantitative risk analysis
ActiveUS7769684B2Improve risk assessmentImprove productivityFinanceResourcesDecision makerManagement process
A semi-quantitative analysis on the risk management process increases the possibility of performing an accurate risks comparison, making easier the identification of which risks shall be prioritized and shall receive the greatest mitigation efforts. Specifically, the semi-quantitative risk analysis enables an improved risks comparison for evaluating the consequences of each risk considering its impacts on the project's Net Present Value (NPV), reflecting the project's cash flow at different times. The use of such method makes the prioritization process more efficient, helping the managers and other personnel involved on the process to focus their efforts to the most critical risks for the project's success. In this sense, the risk management process becomes more efficient and better able to provide better support to the project decision makers.
Owner:ACCENTURE GLOBAL SERVICES LTD
Vanilla-seed fermented milk and formulation, preparation method as well as semiquantitative analysis method thereof
The invention discloses a raw material formulation for vanilla-seed fermented milk, which includes raw milk, lactalbumin powder, a milk-fat product, a sweet substance, a thickening agent, zymogen and vallina-seed jam with viscosity not being higher than 10cm / (20 DEG C, 60s); and the invention discloses a semiquantitative analysis method for addition of the jam, which comprises steps of 1) covering vanilla-seed fermented milk uniformly on an entire filter-paper surface; 2) drying filter-paper surface with the vanilla-seed fermented milk for 15 to 40 minutes; and 3) comparing with a series standard sample. The invention also discloses a preparation method of the vanilla-seed fermented milk, which comprises the steps of: 1, mixing the fermented milk and the vanilla-seed jam to be canned; and 2, implementing the analysis according to the semiquantitative analysis method and fetching the vanilla-seed fermented milk reaching the target addition of the jam. The invention also discloses the vanilla-seed fermented milk prepared through the preparation method. The vanilla-seed fermented milk combines the light fermented flavor of the fermented milk and the fragrance of the vanilla and has rich color, the addition of the jam can be semiquantitatively monitored, the quality of different product batches is stable, and the market potential is remarkable.
Owner:BRIGHT DAIRY & FOOD
Method for rapid detecting sodium sulfoxylate formaldehyde in foodstuff
InactiveCN1737544ARealize detectionNovel methodMaterial analysis by observing effect on chemical indicatorPreparing sample for investigationPhenolphthaleinRedox
This invention relates to one rapid test method of sodium sulfoxylate formaldehyde in food, which comprises the following steps: a, preparing sample and smashing the food to be tested for dipping and filtering for use; b, formaldehyde testing and using the reaction between the formaldehyde and sodium sulfite to generate OH- and according to the characteristics of product, judging by the color developing method of thymol phthalein and semi-determining the content by electrometric method; c, sodium acid sulfite testing: using amidon as indicator and generating Na2SO4 and I- to turning the solution from blue to none to judge whether there is NaHSO3.
Owner:重庆工学院
Method for determining volatile element in shaoxing wine
InactiveCN101021509AImprove qualityAvoid it happening againComponent separationPreparing sample for investigationOrganic solventGas phase
A method to detect volatile component in yellow wine belongs to technical field of yellow wine specialty analysis. The invention adopts coupled technique of roof solid phase extraction and gas phase chromatography-mass spectrum to make qualitative and semi-quantitative analysis of volatile component in yellow wine. The method can detect volatile compound in yellow wine quickly and estimate its proportion in all volatile compound initiatively. Roof solid phase extraction can complete under normal temperature without organic solvent, which avoid producing new compound during analyzing and enrich volatile component effectively. Mass spectrum detection can identify volatile component accurately to improve accuracy of the method. The invention has significance in researching micro constituent in yellow wine and improving its quality.
Owner:JIANGNAN UNIV
Method for detecting multiple anions by using indicator displacement colorimetric sensor array
ActiveCN103123323AHigh sensitivityUniform and stable colorMaterial analysis by observing effect on chemical indicatorPrincipal component analysisColorimetric sensor array
The invention relates to a method for detecting multiple anions in water by using an indicator displacement colorimetric sensor array. The method comprises the following steps of: mixing several colored metal ion chelating agents and metal ion receptors which correspond to the colored metal ion chelating agents under the condition of an aqueous solution to obtain a chelate, so that the indicator displacement colorimetric sensor array is constructed; and displacing metal ions from the colored chelate by means of the precipitation reaction between different anions and metal ions and the effect of the further generation of complexes, so that the change of the color of the chelate is caused. By the method, whether ten kinds of trace amount of anions, namely S<2->, F<->, Cl<->, Br<->, I<->, SO4<2->, PO4<3->, CrO4<2->, NO2<-> and C2O4<2-> in water exceed the national discharge standard of sewage or drinking water can be rapidly determined. In the testing process of a sample, an atlas of the sample is compared with a ''fingerprint atlas'' by mathematical statistics methods such as cluster analysis and principal component analysis, so that an unknown anion is subjected to qualitative or semiquantitative analysis.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Determination method for filthy components of high-voltage insulator
ActiveCN103674990AAccurate measurementPrevention of pollution flashover accidentsMaterial analysis using wave/particle radiationComponent separationFourier transform on finite groupsChemical groups
The invention discloses a determination method for filthy components of a high-voltage insulator. The determination method comprises the specific steps: A, pre-treating a sample; B, qualitatively and semi-quantitatively analyzing, namely determining varieties and corresponding contents of elements in the sample by using an X-ray photoelectron spectroscopy, and determining chemical group varieties in the sample by using a fourier transform infrared spectrometer; and C, quantitatively analyzing, namely carrying out an ICP (Inductively Coupled Plasma) test to determine the content of cations in the sample and carrying out an IC (Integrated Circuit) test to determine the content of anions in the sample. According to the determination method for the filthy components of the high-voltage insulator, the filthy components of the high-voltage insulator can be qualitatively and quantitatively analyzed by organically combining an X-ray photoelectron spectroscopy (XPS) test, a fourier transform infrared spectrometer (FTIR) test, the ICP test and an IC test result; the filthy components of the high-voltage insulator can be accurately determined so as to be good for preventing a pollution flashover accident.
Owner:GUANGZHOU POWER SUPPLY BUREAU GUANGDONG POWER GRID CO LTD
Preparation method for increasing content of total saponin, low polarity saponin and argininyl fructosy glucose in red ginseng
The invention provides a preparation method for increasing the content of total saponin, low polarity saponins and argininyl fructosy glucose in red ginseng. Fresh ginseng is cleaned and sealed in a food steamer of a ginseng steaming box to be steamed for 1.5-9h within the temperature range of 100-120 DEG C; after being steamed, the ginseng is naturally cooled in the ginseng steaming box to be 60 DEG C, and then is taken out and put into a baking box to be dried at 50 DEG C for 4-5 days, so that the red ginseng is obtained. High pressure liquid chromatographic detection proves that the content of the total saponin of the red ginseng is improved by 6.00-238.15% compared with the traditional method; the content of the low polarity saponins Rk5, Rg6, Rk1, Rh4, Rg3 and Rs3 can be remarkably increased; when mass spectrum is used for semiquantitative analysis, the content of the argininyl fructosy glucose is higher than that of the generally processed red ginseng by 7.14-311.6%.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Quick detection method for pyrethroid pesticide residue
InactiveCN1763509AQuick checkDetection suitable forMaterial analysis by observing effect on chemical indicatorChemical methods analysisCyanideDecomposition
The invention discloses a rapid detection method of synthetic pyrethroid pesticide residual, which comprises the following steps: (1) extraction the detected material: putting the synthetic pyrethroid pesticide residual in the reactor; (2) decomposition, gas production reaction: decomposing the detected material into compound within cyanide; adding the solid particle of sodium borohydride and adsorption host to mix with the detected material; adding sulphuric acid solution in the reactor to react large amount of gas; (3) colour reaction: guiding the decomposed gas in the absorber; reacting with the specific developer to make solution display blue; (4) qualitative analysis: generating qualitative or semiquanlitative analysis of synthetic pyrethroid pesticide in comparison with the colorimetric card.
Owner:INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Screening method of volatile organic compound in cigarette filter tip blasting-bead
ActiveCN106770707AReflect intakeAchieving Comprehensive ScreeningComponent separationGas phaseScreening method
The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
Method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables
InactiveCN103675081AFast analysisEasy to measureMaterial analysis by electric/magnetic meansSolventIon-mobility spectrometry
The invention discloses a method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables. With an ion mobility spectrometry technology as a basic detection technology, a quick qualitative and semi-quantitative analysis method for detecting pesticide residues based on an ion mobility spectrometry is established by a positive ion and negative ion mode. The method comprises the following steps: baking sampled sheets containing different pesticide samples for drying solvents, and then, conveying the sampled sheets into an ion mobility spectrometry thermal analysis sample injector to detect and analyze the sampled sheets to obtain detection signals, and performing qualitative and semi-quantitative analysis according to sample peak migration time and signal strength. Limit of detection of pesticide standard measurement can be less than 1ng. The measurement method is simple, convenient, quick, efficient and highly reliable, and can be widely used for analyzing pesticide residues on surfaces of fruits and vegetables in the food safety field.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Semi-quantitative risk analysis
ActiveUS20070271198A1Improve risk assessmentSubstantial manpowerFinanceResourcesDecision makerManagement process
A semi-quantitative analysis on the risk management process increases the possibility of performing an accurate risks comparison, making easier the identification of which risks shall be prioritized and shall receive the greatest mitigation efforts. Specifically, the semi-quantitative risk analysis enables an improved risks comparison for evaluating the consequences of each risk considering its impacts on the project's Net Present Value (NPV), reflecting the project's cash flow at different times. The use of such method makes the prioritization process more efficient, helping the managers and other personnel involved on the process to focus their efforts to the most critical risks for the project's success. In this sense, the risk management process becomes more efficient and better able to provide better support to the project decision makers.
Owner:ACCENTURE GLOBAL SERVICES LTD
Kit for detecting knubble type M2 pyruvate kinase and preparation method thereof
The invention relates to a test kit for detecting tumor M2 pyruvate kinase and a relative preparation method, belonging to biological medicine and medical test technical field. The inventive test kit comprises a bottom plate (1), a colorful particle conjugate pad (3), a test sample pad (4), a water absorption pad (5) and a porous filter membrane for reaction, wherein the conjugate pad is fixed with anti-tumor M2 pyruvate kinase antibody marked by colorful particles, the porous filter membrane is arranged with a test region (a) and a reference region (b), the test region covers anti-tumor M2 pyruvate kinase antibody, the reference region covers anti-mouse IgG or antigen solution. The invention can process semi-quantitatively analyze and measure tumor M2 pyruvate kinase in 10min. The test kit is portable and easy to store, with simple operation, stable method, non additional tester and enzyme and the application for quickly and simply detecting tumor M2 pyruvate kinase on solid membrane medium.
Owner:FUDAN UNIV SHANGHAI CANCER CENT
Immune chromatography test paper detection method based on catalyzing and amplifying of detection signal
InactiveCN103063844AHigh detection sensitivityHigh signal resolutionBiological testingNano catalystChemical plating
The invention discloses an immune chromatography test paper detection method based on catalyzing and amplifying of a detection signal. The immune chromatography test paper detection method comprises the following steps: capturing a molecule to be detected to a detecting site through the chromatographic technique; and catalyzing and amplifying the detection signal under the catalyzing effect. According to the immune chromatography test paper detection method based on catalyzing and amplifying of the detection signal, the biomolecule such as immunoglobulin is marked by a catalytic nanometer material; the detection signal is further amplified through an immune chromatography and a chemical plating technology, so that the sensitivity is improved; a large number of catalytic systems of nano-catalysts are provided, the nano-catalysts can catalyze silver, and also can catalyze the settlement of magnetic materials such as cobalt nickel, so that a magnetic signal can be read while the signal is detected, and as a result, the purposes of quantitative detection and semi-quantitative analysis can be realized.
Owner:SOUTHEAST UNIV
Rapid test kit for benzoyl peroxide (banned additive) in flour and preparation method thereof
InactiveCN102207466ASensitive color reactionMild reaction conditionsMaterial analysis by observing effect on chemical indicatorPositive controlBenzoyl peroxide
The invention discloses a rapid test kit for benzoyl peroxide (banned additive) in flour and a preparation method thereof. The test kit comprises a reaction reagent, a color-developing agent, a positive-control reagent stock solution and a standard colorimetric card; the reaction reagent is a ferrous ion acidic aqueous solution with the concentration of 100mg / L-300mg / L; the color-developing agentis a thiocyanate radical aqueous solution with the concentration of 0.5mol / L-0.75mol / L; the positive-control reagent stock solution is a benzoyl peroxide ethanol solution with the concentration of 500mg / L-1500mg / L; and the standard colorimetric card comprises a number of small comparator blocks, and each comparator block corresponds to a concentration of benzoyl peroxide. According to the invention, after the color of a sample to be tested is developed according to the operation method of the test kit, qualitative and semi-quantitative analyses are directly performed by contrast to the standard colorimetric card, and no large-sized analyzer is used in the entire process of sample analysis, therefore the operation process is simplified, and the time for analysis is shortened.
Owner:JIANGSU VOCATION & TECHNICAL COLLEGE OF FINANCE & ECONOMICS
Novel color-developing agent luminosity analytical method for measuring hydrogen cyanide content in mainstream smoke quickly
InactiveCN102128826AQuick QualificationRapid Quantitative AnalysisMaterial analysis by observing effect on chemical indicatorPotassium cyanideLuminosity
The invention provides a novel color-developing agent luminosity analytical method for measuring hydrogen cyanide content in mainstream smoke quickly, which comprises the following steps of: pretreating a sample in which hydrogen cyanide in the mainstream smoke is collected, adding a color-developing agent, and quantifying by a luminosity analytical method, wherein the color-developing agent is 4-nitrobenzaldehyde and o-dinitrobenzene; preparing potassium cyanide (KCN) solution which is added with the color-developing agent and has different concentrations to make a standard curve; and quantifying the standard curve by an external standard method, adding the color-developing agent in a cigarette absorption liquid sample, and performing photometry detection at wavelength 533 nm by an ultraviolet spectrophotometer. In the method adopting the color-developing agent containing 4-nitrobenzaldehyde-o-dinitrobenzene, the selectivity and sensitivity are high, a reaction is completed instantaneously and the interference resistant capacity is high. In addition, the method is simple in operation, cigarette sidestream smoke can be detected, the HCN in smoke environment also can be analyzed qualitatively and semiquantitatively, and the method is also suitable for field detection.
Owner:中国烟草总公司湖北省公司
Kit for detecting protein glycosylation subtype and detection method thereof
InactiveCN104502611AWide applicabilityAchieve semi-quantitative detectionBiological testingBiotin-streptavidin complexPolystyrene
The invention discloses a kit for detecting protein glycosylation subtype and a detection method thereof. The detection kit comprises agglutinin, confining liquid, a biotin labeled antibody, horse radish peroxidase labeled streptavidin, a 3',3',5',5'-tetramethyl benzidine substrate developing solution, a stop solution and a cleaning buffer solution. The detection method comprises the following steps: fixing the agglutinin onto a polystyrene solid phase carrier to be used for recognizing and binding glycoprotein in the sample based on specific recognition of agglutinin and an oligosaccharide chain, recognizing the glycoprotein needing to be detected by virtue of specific binding of the protein and the antibody thereof, and realizing semi-quantitative analysis of the glycoprotein. According to the detection kit and the detection method disclosed by the invention, semi-quantitative detection of single protein glycosylation subtype can be realized, and the kit is easy and convenient to operate and low in cost.
Owner:THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
Molecular identification method for structure of dominant bacteria in digestive canal of aquatic animal and its special primer
InactiveCN1840694AAvoid uncertaintyThe result is accurateComponent separationMicrobiological testing/measurementMolecular identificationDigestive canal
The disclosed molecular identification method and specific primer with upper / lower primer as SEQ ID No:1 / 2 for dominant flora structure in aquatic animal enteron comprise: 1) obtaining different fragments and contents of enteron as sample; 2)extracting sample total DNA; 3) with former DNA as template, taking PCR amplification; 4) taking denaturized gradient gel electrophoresis to target fragment, obtaining DGGE fingerprint spectrum by Ag dyeing, and setting another sequenching group; 5) taking cluster and semi-quantitative analysis; 6) recovering dominant strip as template for PCR amplification and sequenching; 7) comparing and analyzing the result to determine structure.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
COVID-19 (coronavirus disease 2019) nucleic acid testing method capable of increasing accurate rate
PendingCN111074008AHigh sensitivityImprove detection accuracyMicrobiological testing/measurementAgainst vector-borne diseasesReverse transcriptaseViral test
The invention relates to a COVID-19 (coronavirus disease 2019) nucleic acid testing method capable of increasing the accurate rate. firstly, a reversal reaction is performed in a reversal reaction system, so that RNA of polyA is subjected to reverse transcription under the assistance of reverse transcriptase and oligo (dT) to form cDNA of poly (T); meanwhile, cDNA subjected to reverse transcription is subjected to an RPA reaction for a period of time for further amplification; an amplified product is mixed with a QPCR (real-time quantitative PCR detecting system), and the mixture is subjectedto a chain reaction on a QPCR instrument and then sequentially subjected to denaturation, reannealing and final extended chain circular reaction respectively; fluorescence signal intensity is collected and detected through detection equipment, qualitative or semiquantitative analytic determination on target segments, and finally, the virus detection result of a patient is judged according to obtained different fluorescence signal data. According to the technical scheme, the COVID-19 nucleic acid testing method which is high in specificity, accurate is result, low in false negative probability,capable of monitoring each stage from the early period to the later period of infection of the patient and capable of increasing the accurate rate can be provided.
Owner:南京申基医药科技有限公司
Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application
The invention discloses a semi-quantitative solid-phase reaction detection technology for content of an object to be detected, a preparation method and application. The technology is a semi-quantitative colloidal metal immune chromatographic detection technology capable of directly detecting the content of the object to be detected, in which the sample of the object to be detected is reacted with a standard comparison product of the object to be detected with known concentration under the same reaction system, wherein a solid-phase membrane, a supporting sheet, a developing agent, a sample applicator and the standard comparison product are provided in the technology; various ready-to-use products for detecting the content of the object to be detected can be prepared; and the technology can be effectively used for clinical detection and detection on the content of the object to be detected, such as foods, medicaments, laboratory supplies and the like, and has high application value and market prospect.
Owner:CHANGZHOU BIOWIN BIOPHARM
Large-scale blood plasma sphingolipid profile analysis method based on liquid chromatography-mass spectrometry combination
ActiveCN103884782ALow toxicityGood repeatabilityComponent separationPhospholipidMass spectrometry imaging
The invention discloses a large-scale blood plasma sphingolipid profile analysis method based on liquid chromatography-mass spectrometry combination, which is characterized in that sphingolipid in blood plasma is extracted by double-phase extraction with methyl tertiary butyl ether / methanol / water (MTBE / MeOH / H2O) combined with mild basic hydrolysis. Then rapid semi-quantitative analysis is realized within 15 min by combination of ultra-high performance liquid chromatography-electro-spray ionization-mass spectrometry. In the invention, the MTBE / MeOH / H2O extraction system is simple, rapid, and easy to operate, has less protein interference in the extract, and has good repeatability; interference of high-abundance phospholipid and glyceride in the lipid group is eliminated by hydrolysis; ion inhibition is reduced; therefore, the purpose of detecting low-abundance sphingolipid with high sensitivity is achieved. With the high resolution separation capability of subsequent ultra-high performance liquid chromatography (UHPLC) and the specificity of MRM, effective distinguishing of isomers is realized, and the introduction of complex isotope correction during quantification is avoided, which makes the method more rapid and accurate.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Substance, kit and method used for detecting systemic infection
ActiveCN107312839AEasy to detectImprove featuresMicrobiological testing/measurementBacteroidesIn vivo
The invention relates to a substance, a kit and a method used for detecting systemic infection. The substance is a circulating DNA disintegrated and released in vivo from a pathogenic bacterium. The substance provided by the invention is based on the detection of DNA degraded by the pathogenic bacterium of peripheral blood, objectively reflects that whether the pathogenic bacterium invades into blood circulation, and better helps to distinguish the systemic infection and non-infective diseases. The substance does not depend on the separation and identification of a living bacterium thallus, is not affected by the content of the living bacterium in a blood specimen, is not affected by the influences of the contaminated bacterium in specimen collection process, and is higher in detection sensitivity and specificity. The provided detection kit comprises a capture probe for 16 S rDNA of 662 pathogenic bacteria, and can better identify the species of the pathogenic bacterium. The provided detection method better reflects the severity of the infection as well as the course and the condition of the disease through the semi-quantitative analysis of the circulating DNA from the bacterium in the peripheral blood, so as to guide treatment and evaluate prognosis.
Owner:SUN YAT SEN UNIV CANCER CENT
Test paper based on photosensitive meso-porous silicon-based molecular imprinting microsphere and preparation method thereof
InactiveCN106932391AImprove adsorption capacityImprove smoothnessMaterial analysis by observing effect on chemical indicatorCross-linkIsomerization
The invention discloses a test paper based on a photosensitive meso-porous silicon-based molecular imprinting microsphere and a preparation method thereof. The method comprises the following steps: by taking a mesoporous SiO2 nanosphere with the surface modified by double bond as a carrier, a to-be-detected object as a template molecule, azobenzene compound as a functional monomer, dimethyl acrylate as a cross-linking agent, azo isobutyronitrile as an initiator and acetonitrile as a pore-foaming agent, preparing the photosensitive meso-porous silicon-based molecular imprinting microsphere, and then removing the template molecule with a solvent under a light-operated effect, thereby acquiring the molecular imprinting microsphere. According to the technology, the thickness of the polymer shell of the nuclear shell type molecular imprinting microsphere can be strictly controlled, the regulation optimization of the adsorption capacity can be realized, the binding strength of the polymer shell and the mesoporous silicon core can be enhanced and the mechanical damage of the subsequent process to the molecular imprinting microsphere can be avoided. The test paper prepared according to the invention utilizes the photosensitive isomerization and photochromic principle of the functional monomer of the polymer to realize the photosensitive recognition of the molecular imprinting microsphere for the detected object and semi-quantitative analysis can be performed according to the color depth.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Method for measuring aroma components in main stream smoke of cigarettes
ActiveCN106353419AImprove reliabilityReduce in quantityComponent separationComprehensive two-dimensional gas chromatographyMass spectrometric
The invention discloses a method for measuring aroma components in main stream smoke of cigarettes. The method comprises the following steps: (1) placing a filter disc gathered with total particular matters of the main stream smoke of the cigarettes in an extracting solution and extracting, and filtering to obtain filtrate which is a sample solution, wherein the extracting solution is any one of acetone, dichloromethane and hexamethylene; (2) analyzing the sample solution obtained in the step (1) with a comprehensive two-dimensional gas chromatography-gas chromatograph-mass spectrometer; and (3) qualitatively and semi-quantitatively analyzing smoke components by using GC Image software. By the method, a small amount of cigarettes can be directly collected by using a smoking machine standard method to prepare the sample solution, and a complicated sample pretreatment process is not required. The method has the advantages of quick and efficient analysis, small interference, high resolution ratio, large peak capacity, accurate identification and the like, is simple to operate, and can identify various aroma components. Problems of limited peak capacity, insufficient resolution ratio, severe peak overlapping and the like in the traditional chromatography are avoided, and reliability of mass spectrometric identification is improved.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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