The invention discloses a fresh animal medicinal composition and a method for measuring the content of Chinese medicines of the fresh animal medicinal composition. The method comprises the following steps of: putting the fresh animal medicinal composition and the Chinese medicines into a measuring flask, dissolving by adding water, performing ultrasonic extraction and centrifugation, taking supernate, and sieving the supernate with a microporous filtering film to obtain a free amino acid test sample solution; and weighing the fresh animal medicinal composition and the Chinese medicines, fullydissolving by adding water, then performing ultrasonic extraction and centrifugation, taking the supernate, putting the supernate into an ampoule bottle, adding hydrochloric acid, performing vacuum nitrogenization and hydrolyzation, taking the mixture out, cooling the mixture to room temperature, adding a NaOH solution, uniformly mixing, transferring to the measuring flask, diluting by adding water, and fixing the volume to a scale, uniformly mixing, centrifuging, taking the supernate, and sieving the supernate with the microporous filtering film to obtain the total amino acid test sample solution. A chromatographic column is used for detecting primary amino acid and secondary amino acid. A mobile phase A comprising acetonitrile, methanol and water and a mobile phase B comprising Na2HPO4 of which the pH is 7.8 are used for performing gradient elution. The method is simple in operation and high in measurement resolution and sensitivity, and by the method, 12 to 26 types of amino acids in the fresh animal medicinal composition and the Chinese medicines thereof can be separated in a short time, so the method is a reliable method for measuring the amino acid content.