Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application

Abstract

The invention discloses a semi-quantitative solid-phase reaction detection technology for content of an object to be detected, a preparation method and application. The technology is a semi-quantitative colloidal metal immune chromatographic detection technology capable of directly detecting the content of the object to be detected, in which the sample of the object to be detected is reacted with a standard comparison product of the object to be detected with known concentration under the same reaction system, wherein a solid-phase membrane, a supporting sheet, a developing agent, a sample applicator and the standard comparison product are provided in the technology; various ready-to-use products for detecting the content of the object to be detected can be prepared; and the technology can be effectively used for clinical detection and detection on the content of the object to be detected, such as foods, medicaments, laboratory supplies and the like, and has high application value and market prospect.

Description

[0001] [Technical field] An immune colloidal metal detection technology, especially a colloidal metal immunochromatographic detection technology capable of direct semi-quantitative analysis and its preparation method and application. [Background technique] [0002] Colloidal metal labeling technology is a new type of immunolabeling technology that uses colloidal metal as a tracer marker or chromogenic agent and is applied to antigen-antibody reactions. Because it does not have problems such as endogenous enzyme interference and radioactive isotope contamination, and colloidal metals of different particle sizes can be used for double or even multiple labeling, making the positioning more accurate. Therefore, it has become a new type of labeling technology after luciferin, enzyme, isotope and latex labeling technology. It has been widely used in electron microscopy, flow cytometry, western blotting, in vitro diagnostic reagents, and the manufacture of food and drug quality testi...

AI Technical Summary

Problems solved by technology

[0005] However, if the currently widely used detection technology based on solid-phase carrier reaction is not equipped with corresponding detection equipment and only relies on the naked eye to judge the results, it is still limited to a qualitative level, and can only be judged at a certain detection level. For example, the minimum detectable amount of human chorionic gonadotropin in urine of early pregnancy colloidal gold method detection test paper used for early pregnancy detection is 25U / L, while the concentration of human chorionic gonadotropin in normal urine is below 1U / L, use The minimum detectable amount of blood cardiac troponin I colloidal gold detection reagent for early diagnosis of acute myocardial infarction is 1.5ng / ml, while the normal blood cardiac troponin I concentration is lower than 0.15ng / ml

Therefore, only when there is such a large difference between negative and positive, the existing detection methods relying on naked eye judgment can determine whether the test is positive or negative, but cannot determine its dynamic change results with further clinical diagnosis and treatment value

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