71results about How to "Detection suitable for" patented technology

The invention relates to a kit of latex-enhanced immunoturbidimetry for detecting content of glycocholic acid in blood serum. Specifically, the provided glycocholic acid detection kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 comprises a reaction promotion agent, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; the reagent R2 comprises latex particles combined with a glycocholic acid antibody, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; and the calibrator comprises a preservative, an electrolyte, a stabilizer, glycocholic acid pure products and a buffer solution. The kit for detecting the content of the glycocholic acid in the blood serum, disclosed by the invention, ensures the high sensitivity and wide linear range of a kit by utilizing a method of coating the latex particles by utilizing polyclonal antibodies, also has the advantages of high accuracy, good repeatability, strong specificity, simplicity in operation and the like, and can be applied to a clinical general full automatic biochemical analyzer.

The fluoroboric dye fluorescent probe for cell zinc ion detection operates on inner photoinduced electron transfer (PET) principle. The probe is prepared through connecting fluoroboric dye with 8-site chloromethyl group and bis(2-pyridyl methyl) amine simply. It has exciting wavelength and emitting wavelength in the visible light region, and has excellent chemical and optical stability. The probe series has very low pKa value of 2-3 normally, and has no effect on fluorescent emitting of pH in the range of 4-10. It has high selectivity on zinc ion without interference from Na, K, Ca, Mg, Mn and other metal ions, and can detect zinc ion concentration as low as nanomole level. The present invention has high quantum yield of probe molecule-zinc ion complex. Fluorescent microscopic imaging shows that the probe has high cell permeability and no toxic side effect on cell and is especially suitable for detection of intracellular zinc ion concentration.

The invention provides a biochip capable of simultaneously visually detecting multiple antibiotics, illegal addition agents and biotoxins. The biochip comprises a chip carrier for fixing a group of detection target object antigens, wherein detection target objects are the antibiotics, the illegal addition agents and the biotoxins; the biochip is prepared by using a method; and the method comprises the following steps of: performing sample operation on bovine serum albumin for blank control and the detection target object antigens on the chip carrier through a biochip preparing system, and fixing the bovine serum albumin and the detection target object antigens in a 20-37 DEG C water bath for 0.5-4h, so as to obtain the biochip. The invention also provides a method capable of simultaneously visually detecting the multiple antibiotics, illegal addition agents and biotoxins by utilizing the biochip. The biochip provided by the invention has the advantages that the structure is simple, the preparation process is simple, the cost is low, multiple targets are arranged, the accuracy is high, the sensitivity is high, the precision is high, the detection time is short, the operation is simple and easy, an expensive detecting instrument is not needed, and the biochip is suitable for sieving field large-scale samples.

The invention discloses an asynchronous processing method and device of a business request, and relates to the field of the network technology. The asynchronous processing method and device does not directly successively send the business request to an application server but puts a business request which belongs to a preset business type in a plurality of business requests associated with a data interaction instruction into a message queue for the application server to take out the business request from the message queue to process the business request. In a data interaction process, the feedback of the application server on the business request of the preset business type does not need to be waited so as to improve the efficiency of a data interaction process and improve user experience.

The invention discloses a time-resolved fluorescence immunoassay reagent kit which is used for detecting quinoxalinone-2-carboxylic acid and a detection method of the time-resolved fluorescence immunoassay reagent kit. The reagent kit comprises a porous coated plate, a buffer solution, quinoxalinone-2-carboxylic acid standard, freeze-dried antibodies of quinoxalinone-2-carboxylic acid, europium-marked goat anti mouse antibodies, a cleaning solution and an enhancement solution. By measuring fluorescence intensity of counts per second (cps), the content of quinoxalinone-2-carboxylic acid in samples is calculated by a standard curve. The kit provided by the invention has the advantages of simple structure, easiness in assembly, corrosion resistance, light weight, low cost, wide application and the like. A valve core has good centering performance and can move flexibly and reliably and can play a good water-plugging role.

The invention relates to the chemiluminescent immunoassay, in particular to a quantitative kit for detecting dog IL-6 by adopting homogeneous chemiluminescent immunoassay and a use method thereof. A detection solution adopted by the kit contains DNA1-IL-6 antibody1 conjugate, DNA2-IL-6 antibody2 conjugate, DNA3 for marking acridinium ester (AE), restriction enzyme and graphene oxide; DNA1 and DNA2have six complementary bases, the DNA3 contains two enzyme digestion sites, and has nine bases to complementarily pair with the DNA1 and the DNA2; the DNA3 is adsorbed on the surface of the grapheneoxide through the Pai-Pai stacking effect, and the chemiluminiscence of the AE marked at the tail end is quenched due to the occurring of the CRET. The method is the homogeneous immunoassay method, the operation is simple, and the analysis time is greatly shortened, the measure of the single sample can be accomplished in 5-10min, by combining the ortho-bunting effect and a chemiluminescent molecular beacon, a switch of the graphene quenching mechanism is induced through immunoreaction, thereby releasing a chemiluminiscence signal without washing, separation and purification steps.

The invention discloses a fluorescent probe and a preparation method thereof, and application in detecting superoxide anions. The structural formula of the fluorescent probe is disclosed in the specification. The preparation method comprises the following steps: in an inert gas protective atmosphere, dissolving melamine and caffeic acid in a DMF (N,N-dimethylformamide) and CH2Cl2 mixed solution, adding HOBT (N-hydroxybenzotriazole) and EDC.HCL (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), and stirring to react at room temperature for 8-16 hours; after the reaction, concentrating to remove the solvent, thereby obtaining the fluorescent probe crude product; and separating and purifying the fluorescent probe crude product with a silica gel thin-layer chromatographic sheet to obtain the fluorescent probe pure product. The fluorescent probe has the advantages of novel structure, high sensitivity, high selectivity and high light stability.

This invention is near-infrared fluorescent probe for intracellular zinc ion test. The syncetic process is as followings: A.mix cyanine dyes Cy.7 and complexing group in the weight propotion of 1:5-10, resolve the mix in N,N-dimethyl formamide, and react at 60-70deg C for 2-5 hours with the protection of inactive gas; B. Cool the production A to 10-20deg C, dry blowing with depression, resolve in aether, add water for extraction, the extraction liquor I obtained, then add acetic ester for extraction, the extraction liquor II obtained; C. Add anhydrous addex-magnesium in liquor II for desiccation, dry blowing with depression at 30-40deg C, get rid of acetic ester for the final production. The near-infrared fluorescent probe has a good cellular osmosis and little side effect. In particular, it's seligible for cellular zinc ion test.

The invention relates to a stilbene two-photon fluorescence probe for detecting a zinc ion in a cell. Single-photon excitation and two-photon excitation of a probe molecule are respectively carried out in a range from 390 to 420 nm and in a range from 790 to 820 nm, and chemical-optic stability is good; the fluorescence quantum yield is high before the probe molecule is complexed with the zinc ion, and single-photon detection and two-photon detection on the zinc ion can be carried out; the probe molecule has a good selectivity on the zinc ion; in a physiological pH range, the probe molecule is insensitive to the variation of the pH; in a range of pH5-pH8, pH variation dose not basically influence fluorescence-emission of a complex; dissociation constant between the probe molecule and the zinc ion is in a micromole grade range, the cell zinc ion with micromole concentration can be detected; the probe molecule has a good cell permeability, is suitable for the detection of zinc ion concentration variation in the cell; and distribution fluorescence images or false color proportion fluorescence images of the zinc ion in various active cells or tissues are obtained by a laser confocal fluorescence microscope.

The invention belongs to the technical field of gene engineering, and discloses a primer combination for detecting leukaemia fusion genes, a kit containing the primer combination, and a multiplex nested RT-PCR (reverse transcription-polymerase chain reaction) method for performing leukaemia fusion gene detection by using the primer combination or kit. The method is based on a multiplex nested RT-PCR technique, and thus, is simple and quick and has high sensitivity. Besides, the reasonable primer combination is utilized to effectively avoid interactions among multiple primer pairs, thereby reducing the detection errors. The detection method can be utilized to comprehensively perform qualitative detection on 43 leukaemia fusion genes, thereby saving the reagent consumption and lowering the detection cost. The detection method has wide detection range, and is suitable for detecting mass samples in clinic.

The invention provides a special prime designed according to conservative M genes of influenza virus. By the aid of the principle of specific binding of biotin and streptavidin, biotin-labeled PCR (polymerase chain reaction) products are bound on a microtiter plate, anti-digoxin peroxidase is added, and finally TMB (tetramethylbenzidine) color development solution is added, so that positive products can develop colors. On the basis, a special kit is successfully prepared. The method combines PCR with a high-sensitivity digoxin detection system, the sensitivity of the method is 100 times higher than that of a conventional agarose gel electrophoresis detection method, and the method is high in specificity and overcomes difficulty in detecting AIV (avian influenza virus) of a low content in a tissue sample. Compared with a virus isolation method, the method has the advantages that coincidence rate can reach more than 95%, pollution of chemical agents (such as ethidium bromide) is eliminated, automation is benefited, the method is suitable for detecting a large number of samples, and a new approach is provided for early diagnosis of avian influenza and molecular epidemiological investigation.

The invention discloses a Newcastle disease novel visualized room temperature recombinase ploymerase amplication (RT-RPA) nucleic acid test strip detection kit and applications. The kit contains the nucleotide sequences of primers and a probe which are shown in SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7, and a nucleic acid detection test strip. The application method of the kit includes the following steps: configuring an RT-RPA reaction system, and performing a normal temperature and constant temperature reaction; and directly performing interpretation on a product obtained by the constant temperature reaction after the product is detected by the nucleic acid detection test strip, a positive result appearing two red strips, one being located in a detection area, and the other one being locatedin a quality control area, and a negative result only appearing one red strip in the quality control area, and no red strips appears in the detection area. The method for rapidly detecting Newcastledisease virus can be established by adopting an RPA-nucleic acid test strip technology; and the kit and the method are simple in operation, high in specificity and safe and pollution-free, and are easy to observe reaction results and suitable for clinical site detection.

The invention relates to a high-sensitivity chemiluminescence immune assay method based on analogue enzyme labeled silver nanoparticles. The high-sensitivity chemiluminescence immune assay method based on analogue enzyme labeled silver nanoparticles comprises the following steps: constructing a 4*12 array on an aldehyde substrate by using a manual film adhering technology; preparing an analogue enzyme labeled silver nanoparticle probe by modifying a signal antibody Ab2 and high-proportion G-quadruplex DNA / heme compound on the surfaces of the silver nanoparticles; capturing the analogue enzyme labeled silver nanoparticle probe into a sensing pool by the analogue enzyme labeled silver nanoparticle probe through sandwich immunoreaction; loading a large number of analogue enzymes with characteristics of peroxidase on the silver nanoparticles; and catalyzing hydrogen peroxide oxidized luminol to obtain a high-strength chemiluminescence signal so as to realize high-sensitivity imaging immunoassay of protein. The immunosense method has the advantages of high sensitivity, high flux, good stability, easiness in operation, low cost and the like, and has certain clinical application value.

The invention relates to a diphenylethene group dual cyano benzene two-photon fluorescence probe for detecting and displaying intracellular Hg ions, and the probe is prepared by performing nucleophilic substitution on mercaptoethanol and 2,5-di(4-[di(2-chloroethyl)amino]styryl)para-phthalonitrile. Laser excitation of 810nm can avoid photoinduced toxicity of cells. In a range of pH being 4.5-13, the probe has good selectivity on the Hg ions, and Na, K, Ca, Mg and Mn ions have no interference with detection, and the Hg ions of micro molarity can be detected. Fluorescence intensity is reduced after complexing of probe molecules and the Hg ions, and the content of the Hg ions can be subjected to two-photon fluorescence detection. A two-photon fluorescence microimaging experiment shows that the probe has good permeability on fibroblasts and other cells, has no toxic or side effects on the cells and is particularly suitable for the detection of intracellular Hg ion concentration change and Hg ion distribution.

The invention discloses a chip detection method of visual protein in residual gentamicin in animal derived food and belongs to indirect competitive immune method. The chip detection method is characterized by utilizing a carbodiimide method to further combine gentamicin envelope antigen and create gentamicin in animal derived food. The method includes fixing the gentamicin envelope antigen and an internal standard substance on a chip carrier, then adding free gentamicin and gentamicin monoclonal antibody into a reaction region, conducting competitive reaction between the free gentamicin and conjugate fixed on a chip, conducting washing after reaction is finished, then adding secondary antibody marked by collaurum, conducting washing after reaction is complete, adding silver enhancement chromogenic agent, enabling the collaurum to catalyze silver atoms into visual black silver, scanning images through a scanner and finally achieving in-hole semi-quantitative and quantitative detection simultaneously. By means of the chip detection method, quantity of the residual gentamicin in the animal derived food can be quickly and accurately detected, and the detection method is high in sensitivity, short in detection time and simple and easy to operate.

The invention discloses a test strip kit for detecting nucleic acid of classical swine fever virus through room-temperature recombinase polymerase amplification (RT-RPA) and application thereof. The kit comprises primer pairs with nucleotide sequence as shown in the claim 1 and a nucleic acid test strip. An application method of the kit comprises the following steps: firstly a RT-RPA reaction system is configured for normal-temperature constant-temperature reaction; after detected with the nucleic acid test strip, a product obtained after the constant-temperature reaction directly undergoes interpretation: a positive result is two red strips with one strip positioned in a detection area and the other strip positioned in a quality control area; a negative result is a red strip in the quality control area with no red strip in the detection area. The RPA-nucleic acid test strip technology is adopted for the first time to establish a method for rapid detection of CSFV. The kit of the invention is simple to operate and has high specificity. Reaction results are easy to observe. The kit is safe and pollution-free, and is very suitable for clinic field test.

The invention relates to a homogeneous chemiluminescence immune assay method based on an adjacent position striking effect. A detection solution of the method includes a DNA1-antibody 1 conjugate, a DNA2-antibody 2 conjugate, auxiliary DNA3, auxiliary DNA4, molecular beacon DNA5 and restrictive endonuclease. When a target protein exists, a DNA1-antibody 1 and a DNA2-antibody 2 form a sandwich immune complex to make DNA3 and DNA4 respectively hybridized with DNA1 and DNA2 close to each other in order to form an adjacent position strike complex, the adjacent position strike complex can be hybridized with the DNA5 to open its hairpin structure, and a dye Cy5 goes away from a quencher to generate chemiluminiscence. A double chain formed by the adjacent position strike complex and the DNA5 can be identified by endonuclease, new DNA5 is opened after the DNA5 is cut, and the cycle of above steps can realize onsite amplified luminescence in order to realize the highly-sensitive quantitative analysis of the target protein. The method has the advantages of realization of fast one-step protein detection, simple operation and high universality.

The invention discloses a method for detecting peculiar smell caused by acetic acid and propionic acid in tobacco sheets, which comprises the following steps: (1) weighing tobacco papermaking method sheets with standard weight as a sample for detection; (2) putting the sample to be detected into a top hollow bottle and preheating; (3) keeping the temperature of the top hollow bottle unchanged, purging substances volatilized from the sample to a collecting trap, then blowing dryly and removing water which is previously carried in the collecting trap; (4) heating the collecting trap so as to desorb the collected substances; (5) analyzing the desorbed substances by a gas phase chromatography / mass spectrum united instrument; and (6) obtaining the peak area of the characteristic peak of acetic acid by the integration of a spectrogram so as to characterize the content of the acetic acid; after extracting characteristic ion flow, obtaining the peak area of propionic acid by integration so as to characterize the content of the propionic acid. The invention has the advantages of simple, convenient, quick and accurate operation, strong versatility of instruments and the like.

This invention relates to a near infrared fluorescent probe for detecting hydroxyl free radicals, synthesis method and use thereof. The near-IR fluorescent probe, manufacture method and application for -OH Free Radical --a. adding the 2, 2, 6, 6-tetramethyl peridol and strong base by mole ratio as 1:0.5~2.5 into the DMF to dissolve and water-bath react for 25-40min with IG protection; b. adding Cyanine-7 into the solution by same mole rate with the DMF to obtain the Cyanine-7 solution; adding former mixed liquid into the current solution slowly within 10-20min for reaction 25-35min in dried brash ice; c. vacuum evaporating the product to remove the DMF, cleaning with aether, and vacuum drying to obtain the coarse product; d. separating with silica gel column chromatography for the deep-blue solid pure product.

The invention selects a highly conserved p72 gene of African swine fever viruses as a detection target gene to design specific primers, establish a fluorescent isothermal amplification detection method, and develop a nucleic acid extraction-free detection kit for African swine fever viruses. A fluorescent dye is used for indicating an amplification reaction result, thereby facilitating accurate determination; a nucleic acid extraction-free reaction solution is used for processing samples without extracting the DNA of the samples, thereby simplifying the test operation, reducing the cross contamination and lowering the cost; the whole detection process is fast and only requires 50 min; multiple samples can be detected at one time; a used fluorescent isothermal amplification instrument has low cost; and the kit provided by the invention has the advantages of high amplification efficiency and good specificity, and is suitable for use in on-site and grass-roots units for pig breeding, slaughtering, quarantining and monitoring.

The invention relates to the technical field of disinfection equipment, in particular to intelligent disinfection equipment. The intelligent disinfection equipment comprises an air inlet system, an air outlet system and a disinfection system arranged between the air inlet system and the air outlet system. The intelligent disinfection equipment further comprises a detection device which is suitablefor detecting harmful substances in air in an environment. The detection device is arranged in the intelligent disinfection equipment and can detect harmful substances in air in a surrounding environment. When the detection device detects harmful substances in air, the disinfection equipment is controlled to start disinfecting work and when the detection device detects that no harmful substancesexist in air or the amount of the harmful substances meets a national safety standard, the disinfection equipment is controlled to stop disinfecting. By means of intelligent recognition of the detection device, the working hour of the disinfection equipment is controlled reasonably on the premise of guaranteeing the disinfecting and killing safety, so that the work efficiency is improved and the waste of resources is reduced.

The invention provides a technology for rapidly detecting specific DNA (Deoxyribonucleic Acid) by applying an SYBR Green I fluorescent dye to a test paper strip. The technology comprises the following steps of selecting a nylon membrane as a material of the test paper strip; pointing probes onto the nylon membrane, then, irradiating with ultraviolet rays, baking, drying in the dark and storing, wherein the probes are specific nucleotide sequences to be detected; carrying out PCR (Polymerase Chain Reaction) amplification in a manner of taking samples to be detected as templates and taking primers, which are designed by taking the probes as templates, as primers, then, carrying out denaturation for 10 minutes at the temperature of 94 DEG C, and immediately lowering by 4 DEG C, so as to obtain sequences to be detected; flushing the nylon membrane with a buffer solution, dispensing a hybridization solution to the nylon membrane, hybridizing in the dark at room temperature, cleaning the nylon membrane sequentially with a buffer solution and deionized water, imaging with a 488nm-laser imaging scanner, and judging a result in accordance with whether the nylon membrane emits light or not, wherein the hybridization solution contains SYBR Green I and the sequences to be detected. The technology is simple, inexpensive and rapid and is applicable to clinical rapid diagnosis.

The invention belongs to the technical field of insole detection devices, and particularly disclosed an intelligent insole capable of detecting pes supinatus and pes pronatus different in size. The insole includes an insole body, a processor terminal and an adjusting mechanism; the adjusting mechanism includes a vertical rod, a toe cross rod, a foot arch cross rod, a heel cross rod, a toe connecting block, a foot arch connecting block and a heel connecting block. By adopting the insole, pressure data of six areas of a planta pedis can be detected, judgement of pes supinatus and pes pronatus can be achieved according to the pressure data, and meanwhile, by means of rotary connection between the vertical rod and each cross rod, the size of the insole is changed, one pair of the insoles can be designed for different sizes, and therefore the insole can be used by multiple people to detect different foot sizes, and is convenient to operate, capable of saving the cost and suitable for detection in hospitals and the like.

The invention discloses a method for detecting the clenbuterol content in an animal derived sample quickly in site. According to the method, clenbuterol is used as a detection target, a method integrating 96-well plate enzyme-linked immunosorbent assay and silk-screen printing electrode quick detection is established, and the purpose of detecting the clenbuterol content in the animal derived sample quantitatively is realized. By means of the method, the residual amount of clenbuterol in the animal derived sample can be detected quickly and accurately, the sensitivity is high, the detecting time is short, operation is simple and easy to implement and the requirement for quick and high-flux clenbuterol screening is met.

The invention provides an amikacin and kanamycin two-in-one fast detection enzyme linked immunosorbent assay kit, which comprises an enzyme labeling plate, an enzyme labeling antibody, a amikacin standard product, substrate color developing liquid, terminating liquid, washing liquid and an amikacin antibody, wherein amikacin hapten-protein conjugates are coated on the enzyme labeling plate. The kit disclosed by the invention can be used for qualitative and quantitative detection of residue of amikacin and kanamycin in egg and chicken samples. According to the method for detecting amikacin and kanamycin in the egg and chicken sample, firstly, the egg and chicken sample to be tested is treated; then, the enzyme linked immunosorbent assay kit is used for detection; finally, a detection result is analyzed. The enzyme linked immunosorbent assay kit provided by the invention has the advantages that the structure is simple; the use is convenient; the carrying is convenient; the detection method is efficient, accurate, simple and convenient; the kit is suitable for the qualitative and quantitative detection of mass samples.

The invention provides a loop-mediated isothermal amplification quick detecting kit for vibrio harveyi. The loop-mediated isothermal amplification quick detecting kit comprises one reactive liquid A, wherein the reactive liquid A comprises the following components: 10 * Lampbuffer, dNTP, a magnesium sulfate water solution, a glycine betaine water solution, a primer vh-F3, vh-B3, vh-FIP, vh-BIP, Bst DNA (Deoxyribose Nucleic Acid)enzyme, 10 * fluorochrome SYBRGreen I, a positive control sample, a negative control sample and ultrapure water. The invention also relates to a detecting method using the kit. The kit has the advantages of high specificity, high sensitivity, fast speed, low cost and simpler operation method, and is suitable for quickly detecting an aquaculture site. With adoption of the kit and the method, defects of long time, high workload, cross contamination, complex in operation and demands on complex instruments in the prior art can be solved; a new technical platform is provided for detecting diseases of aquatic livestock; and urgent needs on site detecting of diseases caused by the vibrio harveyi at present can be met better.

The invention relates to a quantitative detection method for phthalate plasticizers in dairy products, comprising the following steps: a sample extraction step, a purification step, a preparation step of standard series solutions and a quantitative determination step. According to the quantitative detection method of phthalate plasticizers in dairy products of the present invention, the content of phthalate plasticizers in dairy products can be detected conveniently and quickly, the operation is simple, the practicability is strong, and it is suitable for Detection of plasticizers in general dairy products.

The invention provides a chemiluminescence-based biochip capable of simultaneously detecting various pesticide and veterinary drug residues. The biochip comprises a chip carrier where a set of detecting target antigens is fixed, detecting targets are pesticides and / or veterinary drugs, and the biochip is manufactured through a method comprising the following steps that with bovine serum albumin asblank control, the bovine serum albumin and the detecting target antigens are subjected to point-sampling operation on the chip carrier through a biochip manufacturing system, then incubation in a water bath of 20-37 DEG C is carried out for 0.5-4 h, and the biochip can be obtained after washing and air-drying. The invention further provides a method for detecting various pesticides and veterinary drugs through chemiluminescence and the biochip. The biochip is simple in structure and manufacturing process, low in cost, large in target quantity, high in accuracy, sensitivity and precision, short in detection time and easy and practicable to operate, no expensive detecting instrument is needed, and the biochip is suitable for primary screening of a large scale of samples on site.