Newcastle disease novel visualized room temperature recombinase ploymerase amplication nucleic acid test strip detection kit and applications

Abstract

The invention discloses a Newcastle disease novel visualized room temperature recombinase ploymerase amplication (RT-RPA) nucleic acid test strip detection kit and applications. The kit contains the nucleotide sequences of primers and a probe which are shown in SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7, and a nucleic acid detection test strip. The application method of the kit includes the following steps: configuring an RT-RPA reaction system, and performing a normal temperature and constant temperature reaction; and directly performing interpretation on a product obtained by the constant temperature reaction after the product is detected by the nucleic acid detection test strip, a positive result appearing two red strips, one being located in a detection area, and the other one being locatedin a quality control area, and a negative result only appearing one red strip in the quality control area, and no red strips appears in the detection area. The method for rapidly detecting Newcastledisease virus can be established by adopting an RPA-nucleic acid test strip technology; and the kit and the method are simple in operation, high in specificity and safe and pollution-free, and are easy to observe reaction results and suitable for clinical site detection.

Description

technical field [0001] The invention belongs to the field of preventive veterinary inspection, relates to a test kit for detecting Newcastle disease virus and its application, in particular to a test strip RPA kit for rapid detection of Newcastle disease virus based on RPA reaction, and also relates to a test kit for rapid detection of Newcastle disease virus. Use in the detection of Newcastle disease virus. . Background technique [0002] Newcastle disease (Newcastle Disease) is an acute, febrile, septic and highly contagious poultry infectious disease caused by a type I paramyxovirus-Newcastle disease virus (NDV), which seriously endangers the health of poultry. Both morbidity and mortality are high. It is one of the most serious poultry infectious diseases in the world today, and is listed as a notifiable infectious disease by the World Organization for Animal Health (OIE). Most of the traditional detection methods have shortcomings such as poor specificity and sensiti...

AI Technical Summary

Problems solved by technology

However, molecular biology methods such as RT-PCR and fluorescent quantitative PCR require expensive equipment and professional technicians to operate, and it is difficult to meet the detection requirements in non-laboratory and other grassroots field environments.

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