Method for detecting clenbuterol content in animal derived sample quickly in site

A technology for detecting animals and samples, applied in measuring devices, material inspection products, biological testing, etc., can solve the problems of large size and unsuitable for rapid detection, and achieve the effects of simple preparation, easy mass production, and low cost

Active Publication Date: 2014-01-22
NANJING XIANGZHONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the instruments used in enzyme-linked immunoassays are optical microplate readers, which are large in size and are generally not suitable for carrying on-site rapid detection.

Method used

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  • Method for detecting clenbuterol content in animal derived sample quickly in site
  • Method for detecting clenbuterol content in animal derived sample quickly in site
  • Method for detecting clenbuterol content in animal derived sample quickly in site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Clenbuterol antigen standard (China Veterinary Drug Administration); clenbuterol coated antigen and antibody, clenbuterol ELISA detection kit (Nanjing Xiangzhong Biotechnology Co., Ltd.); HRP-labeled goat anti-mouse IgG (Beijing Boaosen Biological Company); 3,3',5,5'-tetramethylbenzidine (TMB) (Shenggong Bio); urea hydrogen peroxide (CO(NH 2 )·H 2 o 2 ) (Tianjin Xiens Biological Company); sulfuric acid, methanol, concentrated hydrochloric acid, sodium chloride (analytical grade) (Nanjing Chemical Reagent Company); MΩ cm); pork (purchased from a farmer’s market in Nanjing); pig urine (from a pig farm in Nanjing); screen-printed electrodes (the working electrode and auxiliary electrode were printed with carbon paste, and the reference electrode was silver / chlorine Silver paste printing, Nanjing Xiangzhong Biotechnology Co., Ltd.). Example 1: A method for rapid on-site detection of clenbuterol based on screen-printed electrodes.

[0030] (1) Test sample processing:

...

Embodiment 2

[0046] Example 2: A method for rapid on-site detection of clenbuterol based on screen-printed electrodes.

[0047] In the present embodiment, the processing of the detection sample and the preparation method of the screen printing electrode are the same as in the embodiment 1, the difference is that the steps of the indirect competitive ELISA reaction in the present embodiment are as follows:

[0048] (1) Add 200 μL clenbuterol antibody and free clenbuterol standard solution of different concentrations to some reaction wells, add 200 μL clenbuterol antibody and sample solution (pork and pig urine sample solution) to the remaining reaction wells ), all reaction wells were reacted in a water bath at 37°C for 1 h, then washed 5 times with a plate washer, and drained on absorbent paper.

[0049] (2) Add 200 μL of HRP-labeled goat anti-mouse IgG to each reaction well, react in a water bath at 37°C for 1 hour, then wash 5 times with a plate washer, and drain on absorbent paper.

[...

Embodiment 3

[0052] Example 3: A method for rapid on-site detection of clenbuterol based on screen-printed electrodes.

[0053] In the present embodiment, the processing of the detection sample and the preparation method of the screen printing electrode are the same as in the embodiment 1, the difference is that the steps of the indirect competitive ELISA reaction in the present embodiment are as follows:

[0054] (1) Add 50 μL of clenbuterol antibody and free clenbuterol standard solution of different concentrations to some reaction wells, add 50 μL of clenbuterol antibody and sample solution (pork and pig urine sample solution) to the remaining reaction wells ), all reaction wells were reacted in a water bath at 37°C for 3 h, then washed 5 times with a plate washer, and drained on absorbent paper.

[0055] (2) Add 50 μL of HRP-labeled goat anti-mouse IgG to each reaction well, react in a water bath at 37°C for 3 hours, then wash 5 times with a plate washer, and drain on absorbent paper. ...

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Abstract

The invention discloses a method for detecting the clenbuterol content in an animal derived sample quickly in site. According to the method, clenbuterol is used as a detection target, a method integrating 96-well plate enzyme-linked immunosorbent assay and silk-screen printing electrode quick detection is established, and the purpose of detecting the clenbuterol content in the animal derived sample quantitatively is realized. By means of the method, the residual amount of clenbuterol in the animal derived sample can be detected quickly and accurately, the sensitivity is high, the detecting time is short, operation is simple and easy to implement and the requirement for quick and high-flux clenbuterol screening is met.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analysis and electrochemical immunoassay, and in particular relates to the application of a screen-printed electrode in rapid on-site detection of clenbuterol in animal-derived samples. Background technique [0002] Clenbuterol, commonly known as clenbuterol, is a β-stimulant. In recent years, it has been illegally added to feed to promote the growth of animal skeletal muscle and increase the lean meat rate, because the additive dose is 5-10 times the therapeutic dose. times, and the residual amount in animals is too high to bring harm to consumers, so the detection of clenbuterol in food is particularly important. At present, the detection methods of clenbuterol mainly include chromatography and immunoassay. Chromatography is mainly based on high performance liquid chromatography, because of its sensitivity and accuracy, it can be used as a laboratory confirmation method. Howe...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N27/26G01N27/30
CPCG01N33/5438G01N33/94
Inventor 梁桦李周敏张立柱
Owner NANJING XIANGZHONG BIOTECH
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