Indirect iodometry dynamics spectrophotometry for measuring vitamin C

A technology of vitamins and kinetics, applied in the field of analytical chemistry, to achieve the effect of low analysis cost, less usage, and good linearity

Inactive Publication Date: 2015-11-18
SICHUAN AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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The method involved in the present invention has no report at home and abroad

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  • Indirect iodometry dynamics spectrophotometry for measuring vitamin C
  • Indirect iodometry dynamics spectrophotometry for measuring vitamin C
  • Indirect iodometry dynamics spectrophotometry for measuring vitamin C

Examples

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Embodiment 1

[0030] Embodiment 1: A kind of indirect iodine dynamic photometry precision test and the establishment of standard curve of measuring vitamin C, its operation steps:

[0031] (1) At room temperature (26.9°C), take a quartz cuvette with a volume of 4mL and an optical path of 1cm as the reaction vessel. The total volume of the reaction liquid is 3.30mL, leaving 0.5mL space for shaking; add 1.00mL of 200μg / mL vitamin C standard solution , add 2.00mL of 0.5% starch solution to the cuvette, add 0.20mL of 0.200mg / mL potassium iodate solution, add 0.10mL of 0.15mol / L sulfuric acid solution, shake it up immediately, quickly place it in the photometer, and use The reagent is used as a blank, and the wavelength of 600nm is used as the measurement wavelength. Immediately perform a dynamic photometric scan to obtain the peak absorbance A max ;Parallel 6 times according to this method, with 6 times of determination Amax value calculation, RSD (%)﹤1%.

[0032] The indirect iodine dynam...

Embodiment 2

[0035] Embodiment 2: A kind of indirect iodine dynamic spectrophotometric method for measuring vitamin C measures the vitamin C content of commercially available vitamin C samples, and its operation steps:

[0036] (1) Take 2 commercially available vitamin C samples (tablets), grind them into powder, weigh 0.5000g of the powder sample, use 2% oxalic acid to assist in ultrasonic dissolution and constant volume, filter, take 1.00mL of the filtrate into a 50mL volumetric flask, Distilled water was fixed to volume for testing.

[0037] (2) Take a quartz cuvette with a volume of 4mL and an optical path of 1cm as a reaction container, transfer 1.00mL of the solution to be tested, 2.00mL of 1% starch solution, and use the same amount of the remaining reagents as measured by the method of the present invention A max value (26.9°C), parallel 6 times, press figure 2 The working curve to calculate the content, the measurement results: the vitamin C content of commercially available vi...

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Abstract

The invention belongs to the field of analytical chemistry, and particularly discloses indirect iodometry dynamics spectrophotometry for measuring vitamin C. The spectrophotometry specifically comprises steps as follows: at the room temperature, using a cuvette of 1 cm as a reaction container, adding 0.50-1.40 mL of a 200 mu g / mL vitamin C standard solution and 1.60-2.50 mL of a 0.5% starch solution to enable the volume of the mixed solution to be 3.00 mL, adding 0.20 mL of a 0.200 mg / mL potassium iodate solution and 0.10 mL of a 0.15 mol / L of sulfuric acid solution, evenly shaking the mixture, quickly placing the mixture into a reagent blank for a photometer, performing dynamics spectrophotometry scanning at the wavelength of 600 nm to measure the peak absorbance within a short time, wherein the peak absorbance has a good linear relation with the concentration of the vitamin C. The spectrophotometry has the advantages that the spectrophotometry is convenient, accurate, micro and quick, used drugs are low in cost, easy to obtain, non-toxic, pollution-free and environment-friendly and the like, and is used for measuring the vitamin C in biological samples, drug samples and the like.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to an indirect iodine dynamic photometry method for determining vitamin C. Background technique [0002] The application of indirect iodometric method in volumetric analysis can be regarded as the classic of chemical analysis. The I generated by indirect iodometric method is 2 Forming a chromogenic system with starch solution combined with photometric method for quantitative analysis can realize microanalysis by indirect iodometric method, which makes the analysis operation simple, improves analysis efficiency and reduces analysis cost. At present, the patent documents related to this type of analysis method include: Zhen Hua et al. published a stable iodine-starch color system in "A Spectrophotometric Quantitative Analysis Method for Iodine-Starch Color System" (China, CN103439279B[P]). method and use spectrophotometry for quantitative analysis. Zhen Hua et al. published an ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/38
Inventor 甄铧张学毅
Owner SICHUAN AGRI UNIV
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