Test method for determination of total sugar content in chlortetracycline fermentation broth by refractometer

Abstract

The invention relates to a method for measuring the total sugar content of a chlortetracycline fermentation broth with a refractometer, and the method is characterized by accurate measuring result and low cost. The method comprises the steps of: (1) collecting original data: measuring the sugar content data of a standard sample by iodimetry, with the sugar content of the sample at a gradient concentration of 0-17%; (2) inputting the data measured in step (1) into the refractometer; (3) dropping the sample with sugar content of 0-17% for measuring directly in a determination analysis pool so as to conduct measuring. The method of the invention has the advantages of accurate measuring result and fast detection speed. Meanwhile, after one time of standard data input, no input and detection through iodometry are needed once again during sugar content detection of the sample at a later stage, so that a lot of reagents can be saved. The method of iodometry is complicated for operation, while the adoption of a refractometer is simple in operation. The employment of a refractometer for detection reduces the labor intensity of technicists and improves the working efficiency.

Description

technical field [0001] The invention relates to the field of chemical sample detection, in particular to a test method for measuring the total sugar content of chlortetracycline fermentation broth with a refractometer. Background technique [0002] At present, the traditional iodometric method has been used for the detection of total sugar in aureomycin fermented liquid for many years, which not only requires a large workload, but also takes a long time to measure, and cannot provide accurate and reliable analysis data for production in time. And the cost of determination is higher, adopt iodometric method to measure the total sugar of chlortetracycline fermentation liquid, its determination reagent is expensive. Simultaneously, the waste liquid used in the determination will also pollute the environment, and sometimes in order to reduce the cost, it is often concluded that only one measurement is made, and the error rate of the measurement results is high, which cannot meet...

AI Technical Summary

Problems solved by technology

[0002] The traditional iodometric method has been used for many years for the detection of total sugar in feed aureomycin fermented broth, which not only requires a lot of work, but also takes a long time to measure, and cannot provide accurate and reliable analysis data for production in time.

And the cost of determination is higher, adopt iodometric method to measure the total sugar of chlortetracycline fermentation broth, its determination reagent is expensive

Simultaneously, the waste liquid used in the determination will also pollute the environment, and sometimes in order to reduce the cost, it is often concluded that only one measurement is made, and the error rate of the measurement results is high, which cannot meet the analysis and measurement requirements. Low, direct detection by refractometer, the measurement results also contain deviations, which cannot meet the requirements of analysis and determination

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