40 results about "Cartilage bone" patented technology

Repairs of cartilage defects or of cartilage / bone defects in human or animal joints with the help of devices including a bone part (1), a cartilage layer (2) and a subchondral bone plate (4) or an imitation of such a plate in the transition region between the cartilage layer (2) and the bone part (1). After implantation, the bone part (4) is resorbed and is replaced by reparative tissue only after being essentially totally resorbed. In a critical phase of the healing process, a mechanically inferior cyst is located in the place of the implanted bone part (1). In order to prevent the cartilage layer (2) from sinking into the cyst space during this critical phase of the healing process the device has a top part (11). and a bottom part (12), wherein the top part (11) consists essentially of the cartilage layer (2) and the subchondral bone plate (4) and the bottom part (12) corresponds essentially to the bone part (1) and wherein the top part (11) parallel to the subchondral bone plate (4) has a larger diameter than the bottom part (12). After implantation in a suitable opening or bore (20), the cartilage layer (2) and the subchondral bone plate (4) are supported not only on the bone part (1) but also on native bone tissue having a loading capacity not changing during the healing process. Therefore, the implanted cartilage layer cannot sink during the healing process.

The invention relates to a hollow stent for inducing the regeneration and moulding of cartilage / bone tissues. The structure of the stent comprises an overall three dimensional hollow structure; and a plurality of holes in the surface. The hardness should meet the requirements of cartilage / bone tissues in the repaired parts. The stent should have a good biocompatibility and is made of a biologically degradable material. The biologically degradable material can be made into filling and repairing materials such as artificial auricular cartilage stents, artificial nose bridges, and the like, and can also be made into artificial bone stents with various sizes. The prepared stents with special structures can be combined with cultured cartilage / bone cells in vitro and then implanted into human body. Or, small cartilage blocks and cartilage sheets are filled into the hollow stents. The stents can be implanted into human body at first, and then cartilage / bone cells are injected into the stents. The inoculated cartilage / bone cells proliferate and pass down in the hollow three-dimensional stents, bones or cartilage tissues with the shape of the stents gradually form during the period, and tissues with similar functions and shapes can be obtained.

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

The invention discloses a self-assembly collagen template tissue engineering material as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The self-assembly collagen template tissue engineering material is prepared from collagen and polyhydroxy alcohol by cross-linking and solidification through glutamine transaminase. The preparation method is simple, and is convenient to operate, controllable in process parameter and low in cost. The cross-linking by adopting a microbial enzyme is superior to that by adopting other cross-linking agents; the toxic and side effects of, for example, methacrylic anhydride (MA), a photoinitiator and the like to a cell are avoided; the obtained material has better biological activity, biocompatibility and mechanical properties, can be used for constructing a better three-dimensional scaffold system for the growth of the cell, and can be also used for loading a medicine. The material can be used for the generation of new tissue and the repair of injured tissue, and is clinically used for the fields of tissue engineering of cartilage, bones, skin, blood vessels and the like.

The invention relates to a 3D printed porous polyether-ether-ketone stent and a preparation method and application of the stent. The method comprises the following steps: scanning a damaged area at abone joint to obtain corresponding three-dimensional data; conducting modeling according to the obtained three-dimensional data to obtain a filler model capable of filling the damaged area; and performing 3D printing according to the filler model to prepare and obtain the stent. The stent with a porous structure is manufactured by a 3D printing technology, so that the elasticity modulus and hardness of the stent can be reduced, the stent can be matched with biomechanical properties of soft tissues such as cartilage, bone-cartilage, meniscus and tendon bone at bone joints, and the difficulty ofapplying a polyether-ether-ketone material to soft tissue injury repair is solved.

The invention discloses a material combining porous ultrahigh molecular weight polyethylene and bionic cartilage and a manufacturing technology and belongs to biotic implanting materials and manufacturing technologies. The material comprises a dichromic acid oxidation solution, a biotic cartilage material, a grafting solution and a hard-face substrate. The dichromic acid oxidation solution comprises 20% of potassium dichromate and 80% of concentrated sulfuric acid. The biotic cartilage material comprises 15% of polyvinyl alcohol, 82% of deionized water and 3% of nano-hydroxyapatite. The grafting solution comprises 7% of polyvinyl alcohol, 1.5% of concentrated sulfuric acid and 91.5% of deionized water. The hard-face substrate comprises the ultrahigh molecular weight polyethylene and NaC1 powder. The purity of the polyvinyl alcohol is larger than or equal to 99%, the potassium dichromate is of an analytically pure level, the concentrated sulfuric acid is of a mass fraction 98% grade, and the matter is in mass percent. The material combining the porous ultrahigh molecular weight polyethylene and the bionic cartilage and the manufacturing technology have the advantages that on the basis of a human body natural articular bone-cartilage-bone pair matching manner, an artificial joint of the cartilage is manufactured in a bionic mode, the function of the cartilage is replaced to some extent, abrasion and loosening are not prone to happening, the interface lubricity is good, the biological activity is sufficient and the function of replacing the cartilage is truly achieved.

The invention discloses a scaffold for repairing joints. The scaffold is composed of a porous polymer material layer and a porous metal material layer from top to bottom. The porous metal material layer is a gradient porous metal material layer. The scaffold effectively realizes artificial joint simulation and good force transmission, has excellent mechanical properties and good percolation characteristics, is conducive to colonization, differentiation and proliferation of cartilage and bone cells and is an integrated articular cartilage and subchondral bone repair scaffold.

The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises: preparing cartilage decellularized matrix powder, preparing bone tissue decalcified bone matrix powder; preparing gelatin or collagen solution; preparing cartilage-containing decellularized matrix Prepare gelatin or collagen suspension containing decalcified bone matrix; prepare gelatin or collagen suspension containing cartilage decellularized matrix and decalcified bone matrix; construct cartilage-bone by computer-aided design An integrated three-dimensional digital model, which is 3D printed into a cartilage-bone integrated core grid frame; the cartilage-bone integrated grid frame is fixed in a cylindrical mold and perfused with gelatin or collagen suspension containing decalcified bone matrix , prepare the bone phase scaffold, perfuse the gelatin or collagen suspension containing cartilage decellularized matrix and decalcified bone matrix, prepare the junctional phase scaffold, and then perfuse the gelatin or collagen suspension containing cartilage decellularized matrix, prepare the cartilage phase scaffold, make Chondrogenic-osseointegrated three-dimensional cryo-stent.

Disclosed are an allogeneic bone and / or cartilage bone meal and a preparation method and use thereof, comprising the following steps: (1) pulverizing an allogeneic bone and / or cartilage; (2) blending the pulverized allogeneic bone and / or cartilage and a nuclease to conduct an enzymatic reaction; (3) incubating and washing the pulverized allogeneic bone and / or cartilage with a detergent and an optional first chelating agent; (4) optionally conducting a decalcification treatment with a second chelating agent on the pulverized allogeneic bone and / or cartilage; and (5) grinding the allogeneic bone and / or cartilage after same is treated in step (2), step (3) and optional step (4). Also disclosed are a gel composition, biological scaffolds and a biological gel material containing the bone meal prepared from the above-mentioned method, and a preparation method and use thereof.

The invention discloses a cartilage-bone-bone marrow composite tissue structure and method based on 3D printing of living cells. The biological information of bone tissue is collected, input into a computer for bionic modeling, and the preparation is suitable for cartilage, osteogenesis, mesenchymal stem, and blood vessels. Bio-ink for cells such as endothelium; prepare hard materials for printing dense bone areas; prepare various casting agents; inoculate living cells in corresponding bio-inks at a certain density; load various types of printing inks separately 3D printing is carried out after setting the printing parameters in the printer silo, and the printed bone tissue is cultured in a tissue engineering bionic incubator. After a certain period of time, the cast is removed at different times by temperature control, enzyme control or light control. The artificial blood vessels are grafted outside the cartilage-bone-bone marrow composite tissue, and cultured in a tissue engineering bionic incubator with continuous cycle perfusion to realize the pre-maturation of the printed tissue. It realizes the long-term survival and biological function of 3D printed bone tissue.

Shown and described is an insulin-mimetic as an inductive agent for cartilage and / or bone repair is disclosed. Sodium tungstate (Na2WO4) was utilized as an inductive factor to enhance human mesenchymal stem cell (MSC) chondrogenesis. The chondrogenic differentiation of MSCs was enhanced in the presence of low concentrations of Na2WO4 as compared to control, without Na2WO4. Also disclosed is a composite scaffold capable of supporting cell and tissue growth. The composite scaffold could include zinc oxide and polycaprolactone.

The present invention provides an osteochondral microfluidic chip culture device and an osteochondral microfluidic chip analysis device containing the same; the osteochondral microfluidic chip culture device includes an osteochondral culture layer and communicates with the osteochondral culture layer The nutrient solution channel and the cell metabolic fluid channel connected with the osteochondral culture layer. The osteochondral microfluidic chip culture equipment provided by the present invention realizes the construction of the three-layer structure of cartilage, bone and calcified layer and the subsequent culture process, fully embodies the characteristics of bionics, can simulate the real osteochondral growth environment, and can simultaneously realize bone and cartilage regeneration and subsequent analysis.

The invention discloses a cartilage-bone repair support with bionic gradient and a preparation method thereof. For the cartilage-bone repair support, a cartilage layer of an interwoven porous structure is prepared from sodium hyaluronate and chitosan, a subchondral bone layer of a porous structure is prepared from graphene oxide, chitosan and nano-hydroxyapatite, and structure, composition and function bionics of natural articular cartilage is realized by combining a coprecipitation method and a gradient refrigeration technology by referring to an in-situ bionic thorough. The obtained supporthas an upper layer of structure and a lower layer of structure, nano-hydroxyapatite presents change of bionic gradient distribution which is gradually increased from the cartilage layer to the subchondral bone layer; meanwhile, the cartilage layer in the upper layer is of a mutually penetrating interwoven hierarchical pore structure, the subchondral bone layer is of an array microtube structure which is axially arranged and is vertically through, the support material is excellent in mechanical performance, strong in interface bonding property and good in biological performance, and is expectedto become a novel composite material for treating cartilage-bone defect.