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Injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading

A technology of hydroxybutyrate and porous microspheres, applied in the field of medicine

Inactive Publication Date: 2021-05-18
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports relying on a single biomaterial to induce endochondral ossification to promote bone defect regeneration.

Method used

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  • Injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading
  • Injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading
  • Injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 prepares small-aperture poly(4-hydroxybutyrate) microspheres

[0031] Weigh 100 mg of poly(4-hydroxybutyrate), with a weight average molecular weight of 80,000 g / mol, and add 2 mL of chloroform to dissolve it to obtain solution A.

[0032] Weigh 10 mg of polyethylene glycol with a molecular weight of 1000 g / mol, add 1 mL of ultrapure water to dissolve it to obtain solution B.

[0033] The solutions A and B were mixed and homogenized for 5 min to obtain the primary emulsion.

[0034] Pour the primary emulsion into an aqueous solution of polyvinyl alcohol (1%) and stir to evaporate the solvent, freeze-dry after centrifugation, and sterilize and encapsulate to finally obtain a small-pore poly(4-hydroxybutyrate) microsphere preparation with a particle size of 100- 200μm, the average pore size is 2-10μm.

Embodiment 2

[0035] Embodiment 2 prepares macroporous poly(4-hydroxybutyrate) microspheres

[0036] Weigh 200 mg of poly(4-hydroxybutyrate), with a weight average molecular weight of 100,000 g / mol, add 4 mL of chloroform to dissolve it to obtain solution A.

[0037] Weigh 20 mg of ammonium bicarbonate and add 2 mL of ultrapure water to dissolve it to obtain solution B.

[0038] The solutions A and B were mixed and homogenized for 5 min to obtain the primary emulsion.

[0039] Pour the primary emulsion into polyvinyl alcohol (1%) aqueous solution, stir to evaporate the solvent, freeze-dry after centrifugation.

[0040] The porous poly(4-hydroxybutyrate) microspheres were hydrolyzed with sodium hydroxide (0.1M) solution, washed with ultrapure water, centrifuged, freeze-dried, sterilized and encapsulated to obtain macroporous poly(4- Hydroxybutyrate) microsphere preparation, the particle size is 300-500 μm, and the average pore size is 20-50 μm.

Embodiment 3

[0041] Example 3 Preparation of porous polyethylene glycol-poly(4-hydroxybutyrate) diblock copolymer microspheres

[0042] Weigh 200 mg of polyethylene glycol-poly(4-hydroxybutyrate) diblock copolymer with a number average molecular weight of 9300 g / mol, add 2 mL of chloroform to dissolve it to obtain solution A.

[0043] Weigh 10 mg of ammonium bicarbonate and add 1 mL of ultrapure water to dissolve it to obtain solution B.

[0044] The solutions A and B were mixed and homogenized for 10 min to obtain the primary emulsion.

[0045] Pour the primary emulsion into an aqueous solution of polyvinyl alcohol (2%), stir to evaporate the solvent, freeze-dry after centrifugation, and sterilize and package to obtain polyethylene glycol-poly(4-hydroxybutyrate) diblock copolymer porous microspheres The preparation has a particle size of 50-100 μm and an average pore size of 2-10 μm.

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Abstract

The invention discloses an injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation without stem cell or growth factor loading. The injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation is a porous microsphere constructed by poly(4-hydroxybutyrate) and a copolymer thereof through a pore-foaming agent. The invention further provides a preparation method of the microsphere and application of the microsphere in bone defect repairing. The prepared porous microsphere has the mechanical property similar to that of an extracellular matrix, can be used for enhancing progenitor cell aggregation through the porous structure of the microsphere on the premise of without stem cell or growth factor loading, so that the internal repairing capacity of bones can be supported, and intracartilage ossification bone repairing can be completed through a single material.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an injectable poly(4-hydroxybutyrate) (P4HB) porous microsphere preparation free of stem cells and growth factors. Background technique [0002] Bone defects caused by factors such as trauma, infection, or bone tumors make it difficult for patients to achieve bone repair and healing by themselves. Treatment of such bone defects is a great clinical challenge. Clinically, autologous bone, allogeneic bone or artificial bone materials are often used to repair bone defects. Autogenous bone is the gold standard for clinical treatment of bone defects, but it has disadvantages such as additional trauma, fewer sources, increased blood loss, or increased risk of surgical infection. Although the source of allograft bone is abundant, it will bring about serious immune response, disease cross-infection and slow healing. Artificial bone material is a bone tissue replacement material with s...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/50A61L27/56
CPCA61L27/18A61L27/50A61L27/56A61L2430/02A61L2400/06C08L67/04
Inventor 李志波袁帅帅沈勇阚泽
Owner QINGDAO UNIV OF SCI & TECH
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