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A kind of allogeneic bone powder and its preparation method and application

A technology of allograft and bone powder, which is applied in prosthetics, medical science, etc., can solve the problems of autologous bone autograft transplantation limitations, etc., and achieve enhanced osteogenesis and cartilage repair ability, low calcium, and small particle size. Effect

Active Publication Date: 2019-04-05
蒋青 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the occurrence of complications in the donor site and the shape and size of the autogenous bone are often inconsistent with the defect in the recipient site make autologous bone transplantation limited in the bone field.

Method used

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  • A kind of allogeneic bone powder and its preparation method and application
  • A kind of allogeneic bone powder and its preparation method and application
  • A kind of allogeneic bone powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1. Take 36 long bones from the limbs of New Zealand white rabbits, cut the long bones into bone pieces, wash them, put them into a screw grinder together with liquid nitrogen, and grind them.

[0070] 2. Wash the crushed rabbit bone fragments with distilled water, centrifuge at 3000rpm / min for 5 minutes, and discard the upper liquid.

[0071] 3. Dissolve DNA hydrolase (D4527, Sigma Aldrich, USA) and RNA hydrolase (R5503, Sigma Aldrich, USA) at 0.006wt% and 0.02wt% respectively in sterile phosphate buffered saline (sterile PBS) to prepare solution, and the rabbit bone fragments obtained in step 2 were subjected to an enzymatic reaction at 37°C and incubated for 2-3 hours.

[0072] 4. After the enzymatic reaction is finished, rinse with distilled water and centrifuge at a speed of 500-2000rpm / min for 5 minutes, discard the supernatant, and repeat this step 2-3 times.

[0073] 5. Contain 1% Triton x-100 (polyethylene glycol octyl phenyl ether, X100, Sigma Aldrich, USA) wa...

Embodiment 2

[0080] 1. Take 36 limb long bones of New Zealand white rabbits, cut the long bones into bone pieces and wash them with distilled water.

[0081] 2. Contain 1% Triton x-100 (polyethylene glycol octyl phenyl ether, X100, Sigma Aldrich, USA) washing solution and 1% EDTA chelating agent containing 1% pH by volume configuration Mix the solution and incubate with the rabbit bone fragments obtained in step 1 at 4°C for 72 hours.

[0082] 3. Rinse the bone chips obtained in step 2 thoroughly with distilled water for 2-3 times.

[0083] 4. A solution containing 15% EDTA chelating agent with a pH of 7.2 is configured by volume, and the rabbit bone fragments obtained in step 3 are decalcified with the EDTA chelating agent solution for 120 hours.

[0084] 5. Rinse the bone chips obtained in step 4 thoroughly with distilled water for 2-3 times.

[0085] 6. Dissolve DNA hydrolase (D4527, Sigma Aldrich, USA) and RNA hydrolase (R5503, Sigma Aldrich, USA) at 0.006wt% and 0.02wt% respectively...

Embodiment 3

[0089] 1. Take 8 long bones from the limbs of miniature pigs, cut the long bones into bone pieces, wash them and put them into a screw grinder together with liquid nitrogen to grind them.

[0090] 2. Wash the crushed pork bone fragments with distilled water, centrifuge at 3000rpm / min for 5 minutes, and discard the upper liquid.

[0091] 3. Dissolve DNA hydrolase (D4527, Sigma Aldrich, USA) and RNA hydrolase (R5503, Sigma Aldrich, USA) at 0.006wt% and 0.02wt% respectively in sterile phosphate buffered saline (sterile PBS) to prepare solution, and the porcine bone fragments in step 2 were subjected to an enzymatic reaction at 37°C and incubated for 2-3 hours.

[0092] 4. After the enzymatic reaction, rinse with distilled water and centrifuge at 500-2000rpm / min for 5 minutes, discard the supernatant, and repeat this step 2-3 times.

[0093] 5. Contain 1% Triton x-100 (polyethylene glycol octyl phenyl ether, X100, Sigma Aldrich, USA) washing solution and 1% EDTA chelating agent w...

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Abstract

Disclosed are an allogeneic bone and / or cartilage bone meal and a preparation method and use thereof, comprising the following steps: (1) pulverizing an allogeneic bone and / or cartilage; (2) blending the pulverized allogeneic bone and / or cartilage and a nuclease to conduct an enzymatic reaction; (3) incubating and washing the pulverized allogeneic bone and / or cartilage with a detergent and an optional first chelating agent; (4) optionally conducting a decalcification treatment with a second chelating agent on the pulverized allogeneic bone and / or cartilage; and (5) grinding the allogeneic bone and / or cartilage after same is treated in step (2), step (3) and optional step (4). Also disclosed are a gel composition, biological scaffolds and a biological gel material containing the bone meal prepared from the above-mentioned method, and a preparation method and use thereof.

Description

technical field [0001] This application relates to, but is not limited to, the field of biomaterials. Background technique [0002] Large segmental bone defects caused by trauma, bone tumors, infection, congenital bone defects and other reasons are common and difficult problems in clinical repair and reconstruction surgery. For patients with clinical bone defects, autologous bone transplantation is the best choice to avoid the spread of infectious diseases and severe immune reactions after transplantation. [0003] However, the occurrence of donor site complications and the shape and size of autologous bone are often inconsistent with the defect in the recipient site make autologous bone transplantation limited in the field of bone. Allograft bone is used in the field of bone as an alternative to autologous bone. [0004] At present, related documents related to allograft bone are as follows: CN 200610122058.9, CN 200710107105.7 and CN 201010231771.3, etc. Contents of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/52A61L27/44
CPCA61L27/36A61L27/44A61L27/50A61L27/52
Inventor 蒋青滕华建李澜
Owner 蒋青
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