Osteochondral microfluidic chip culture equipment, osteochondral microfluidic chip analysis equipment including the same

Abstract

The present invention provides an osteochondral microfluidic chip culture device and an osteochondral microfluidic chip analysis device containing the same; the osteochondral microfluidic chip culture device includes an osteochondral culture layer and communicates with the osteochondral culture layer The nutrient solution channel and the cell metabolic fluid channel connected with the osteochondral culture layer. The osteochondral microfluidic chip culture equipment provided by the present invention realizes the construction of the three-layer structure of cartilage, bone and calcified layer and the subsequent culture process, fully embodies the characteristics of bionics, can simulate the real osteochondral growth environment, and can simultaneously realize bone and cartilage regeneration and subsequent analysis.

Description

technical field [0001] The invention belongs to the technical field of culture equipment, and relates to an osteochondral microfluidic chip culture equipment and an osteochondral microfluidic chip analysis equipment comprising the same. Background technique [0002] Osteochondral defect is a common clinical disease, mostly caused by trauma or bone disease, which seriously affects the life of patients. With the deepening of tissue engineering osteochondral research, the integrated construction of composite osteochondral scaffolds has achieved good results in animal experiments, but there is still a lack of efficient analysis of stem cell-induced osteochondral differentiation and osteochondral damage mechanisms. means and research methods. [0003] Based on this, in-depth research on the morphology, structure and physiological functions of normal human osteochondral found that osteochondral includes cartilage, bone and calcified layers. There is no blood, lymph and nerves in...

AI Technical Summary

Problems solved by technology

CN101314765A discloses a method and a bioreactor for culturing artificial cartilage or osteocartilage in vitro with different curved surfaces, including an O 2 , CO 2 Conditions such as temperature regulation, cell culture incubator, and perfusion to keep the culture medium fresh and the addition of active factors to the culture medium will fix the culture blanks with different curved surfaces on the bottom plate of the culture room. During the culture, the culture is simultaneously subjected to The rolling load and the sliding load act at the same time, and the culture is cultivated into cartilage tissue or osteochondral tissue; the culture process is complicated and the conditions are not easy to control

CN104593259A discloses a tissue-engineered osteochondral double-chamber cultivation system, which is composed of a bone cultivation circulation system and a cartilage cultivation circulation system that are respectively connected to the osteochondral double-chamber cultivation chamber and are independent from each other. The osteochondral double-chamber cultivation chamber consists of an external layer chamber, inner layer chamber, osteochondral bionic integrated support, the outer layer chamber and inner layer chamber are separated from each other by the osteochondral bionic integrated support to form mutually independent bone cultivation chamber and cartilage cultivation chamber, the bone The cultivation circulation system communicates with the bone cultivation chamber, and the cartilage cultivation circulation system communicates with the cartilage cultivation chamber, which can be used for in vitro cultivation of tissue-engineered osteochondral, but the cultivated osteochondral lacks a calcified layer, and at the same time, the experimental equipment is too large to Simultaneous cultivation and research of multiple samples in parallel is difficult to achieve real-time observation and analysis for the study of the mechanism of stem cell-induced osteochondral differentiation and osteochondral damage, and because the bionic integrated scaffold is large, the amount of cells that need to be cultured is relatively large. The cycle is too long

Images