The invention discloses a vitrified cryoprotective solution for articular cartilage and a cartilage preservation method. Each 1000ml vitrified cryoprotectant solution contains: 99.9% dimethyl sulfoxide 150-250ml, acetamide 110-200g, 1,2-propylene glycol 70 ~120ml, polyethylene glycol 10~80g, trehalose 20~100g, the rest is deionized water. Soak the osteochondral pieces in gradient vitrification protection solution at 4°C for 10 minutes, seal them in refrigerated tubes, and put them in liquid nitrogen for storage; take them out before transplantation for rewarming, rewarm in a water bath at 37°C for 10 seconds, and then Put them into 10% and 5% sucrose solutions at 37°C successively and stay for 5 minutes each, and finally rinse with phosphate buffer solution for 3 times before transplantation. The invention enables long-term preservation of articular cartilage tissue, maintains a relatively high survival rate of cartilage cells, improves the preservation effect of osteochondral grafts, and is used for cartilage tissue bank and clinical cartilage transplantation.