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Bi-phase bone cartilage repairing support and preparing method thereof

A technique for osteochondral and preparation steps, which is applied in the field of biphasic osteochondral repair scaffolds and its preparation, can solve the problems of complex preparation methods and poor biocompatibility, and achieve good biocompatibility

Active Publication Date: 2016-07-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the osteochondral repair scaffold prepared by the above method can simulate the multilayer structure of cartilage tissue, the preparation method is complicated and the biocompatibility is poor

Method used

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  • Bi-phase bone cartilage repairing support and preparing method thereof
  • Bi-phase bone cartilage repairing support and preparing method thereof

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Experimental program
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Effect test

Embodiment 1

[0026] (1) 1g of chitosan was added to 50ml of 3% (v / v) acrylic acid solution, stirred and reacted at 50°C for 3d, the reaction solution was dialyzed for 3d with a dialysis bag with MWCO of 8000-14000, and then freeze-dried to obtain carboxyethyl shell glycans;

[0027] (2) Dissolve 1g sodium hyaluronate in 100ml deionized water, add 0.535gNaIO 4 , react in the dark at room temperature for 3 hours, add 1.5ml of ethylene glycol to terminate the reaction for 1 hour, dialyze the reaction solution with a dialysis bag with a MWCO of 3500 for 3 days, and freeze-dry to obtain oxidized hyaluronic acid;

[0028] (3) Dissolve carboxyethyl chitosan and oxidized hyaluronic acid in PBS respectively to make a 2% g / mL solution. After fully dissolving, pour the two solutions into a cylindrical mold in equal volume and shake in a vortex. Mix evenly in the container to obtain a cylindrical cartilage layer hydrogel;

[0029] (4) Dissolve carboxyethyl chitosan and oxidized hyaluronic acid in PB...

Embodiment 2

[0033] (1) 1g of chitosan was added to 50ml of 3% (v / v) acrylic acid solution, stirred and reacted at 50°C for 3d, the reaction solution was dialyzed for 3d with a dialysis bag with MWCO of 8000-14000, and then freeze-dried to obtain carboxyethyl shell glycans;

[0034] (2) Dissolve 1g sodium hyaluronate in 100ml deionized water, add 0.535gNaIO 4 , react in the dark at room temperature for 3 hours, add 1.5ml of ethylene glycol to terminate the reaction for 1 hour, dialyze the reaction solution with a dialysis bag with a MWCO of 3500 for 3 days, and freeze-dry to obtain oxidized hyaluronic acid;

[0035] (3) Carboxyethyl chitosan and oxidized hyaluronic acid were dissolved in PBS respectively to form 3% and 5% g / mL solutions. After fully dissolving, the two solutions were poured into cylindrical moulds, Mix evenly in a vortex shaker to obtain a cylindrical cartilage layer hydrogel;

[0036] (4) Dissolve carboxyethyl chitosan and oxidized hyaluronic acid in PBS respectively to...

Embodiment 3

[0038] (1) 1g of chitosan was added to 50ml of 3% (v / v) acrylic acid solution, stirred and reacted at 50°C for 3d, the reaction solution was dialyzed for 3d with a dialysis bag with MWCO of 8000-14000, and then freeze-dried to obtain carboxyethyl shell glycans;

[0039] (2) Dissolve 1g sodium hyaluronate in 100ml deionized water, add 0.535gNaIO 4 , react in the dark at room temperature for 3 hours, add 1.5ml of ethylene glycol to terminate the reaction for 1 hour, dialyze the reaction solution with a dialysis bag with a MWCO of 3500 for 3 days, and freeze-dry to obtain oxidized hyaluronic acid;

[0040] (3) Dissolve the sterilized carboxyethyl chitosan and oxidized hyaluronic acid in sterile PBS respectively to prepare 3% and 5% g / mL solutions. After fully dissolving, the mouse bone marrow Stem cells (ATCC, CRL-2623) were added to the carboxyethyl chitosan solution, the two solutions were poured into a cylindrical mold in equal volume, and mixed evenly in a vortex shaker to o...

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Abstract

The invention belongs to the technical field of biomedical materials and discloses a bi-phase bone cartilage repairing support and a preparing method thereof.The preparing method comprises the steps that chitosan and hyaluronic acid are modified to obtain carboxyethyl-chitosan and oxidation hyaluronic acid, carboxyethyl-chitosan and oxidation hyaluronic acid are solved in a PBS buffer solution, then the obtained two solutions are mixed to obtain uniform cartilage layer hydrogel, nano-hydroxyapatite is added into the carboxyethyl-chitosan solution and the oxidation hyaluronic acid solution, and after ultrasonic dispersion and uniforming, the two solutions are mixed to obtain the uniform subchondral bone layer hydrogel; finally, the cartilage layer hydrogel and the subchondral bone layer hydrogel are transversely incised, new interfaces of the two kinds of hydrogel are glued to obtain the bi-phase bone cartilage repairing support.According to the preparing method, the biocompatibility of the adopted materials is good, operation is easy, and the cartilage layer hydrogel and the subchondral bone layer hydrogel can be firmly combined.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a two-phase osteochondral repair bracket and a preparation method thereof. Background technique [0002] Articular cartilage is a highly differentiated connective tissue that absorbs stress, dampens shock, and provides a low-friction environment for joints. However, trauma and degenerative osteoarthritis easily cause articular cartilage defects, and with the arrival of an aging society, the incidence rate is increasing year by year. Articular cartilage defects include upper cartilage defects, full-thickness cartilage defects and osteochondral defects. Due to the characteristics of articular cartilage without blood vessels, nerves, and low metabolism, it is difficult to supply nutrients, which makes the ability of cartilage tissue to repair itself very limited. [0003] The current clinical methods for treating cartilage defects include: bone plate drill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/50A61L27/52
CPCA61L27/12A61L27/20A61L27/50A61L27/52A61L2430/06C08L5/08
Inventor 曹晓东游柏浩董华李庆涛
Owner SOUTH CHINA UNIV OF TECH
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