Production method of calcified layer-containing bionic tissue engineering bone cartilage integrated support
A technology of tissue engineering bone and calcified cartilage, which is applied in the field of medical materials, can solve the problems of poor repair tissue mechanical properties, poor mechanical properties, poor cartilage integration, etc., and achieve increased osteoinductivity, increased stiffness, and good bone tissue biophase capacitive effect
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Embodiment 1
[0073] A method for preparing a biomimetic tissue-engineered osteochondral integrated scaffold containing a calcified layer. The prepared hyaline cartilage layer, calcified cartilage layer and subchondral bone layer are vacuum-heated and granulated into a dense calcified cartilage layer and two layers. On the side is a composite scaffold integrating porous hyaline cartilage layer and subchondral bone layer.
[0074] among them,
[0075] Using polylactic acid (PLLA) as the matrix, calcium polyphosphate short fibers (CPPf) as the reinforcement, and sodium chloride as the porogen, the hyaline cartilage layer and subchondral bone layer are prepared by solvent casting method;
[0076] Using polylactic acid (PLLA) as the matrix and calcium polyphosphate long fibers (CPPf) as the reinforcement, a dense calcified cartilage layer (CPPf / PLLA film) is prepared by the film lay-up method.
[0077] The calcified cartilage layer film is prepared according to the following steps:
[0078] Select ...
Embodiment 2
[0098] A preparation method of a biomimetic tissue-engineered osteochondral integrated scaffold containing calcification layer is basically the same as that in Example 1, except that:
[0099] The weight ratio of calcium polyphosphate fiber: polylactic acid is 40:60. The viscosity average molecular weight of the polylactic acid is 500,000. The daily degradation rate of the calcium polyphosphate fiber is 4%. The particle size of the porogen NaCl is 800 μm.
[0100] The preparation of the calcified cartilage layer film is the same as in Example 1.
[0101] The preparation of the hyaline cartilage layer and subchondral bone layer is the same as in Example 1.
[0102] When preparing the stent by lamination, the specific process parameters are: temperature 200°C, holding time 3h, pressure 12MPa, and pressure holding time 12min.
Embodiment 3
[0104] A preparation method of a biomimetic tissue-engineered osteochondral integrated scaffold containing a calcified layer is basically the same as that in Example 1, except that:
[0105] The weight ratio of calcium polyphosphate fiber: polylactic acid is 30:70. The viscosity average molecular weight of the polylactic acid is 400,000. The daily degradation rate of the calcium polyphosphate fiber is 0.8%. The particle size of the porogen NaCl is 100 μm.
[0106] The preparation of the calcified cartilage layer film is the same as in Example 1.
[0107] The preparation of the hyaline cartilage layer and subchondral bone layer is the same as in Example 1.
[0108] When preparing the stent by lamination, the specific process parameters are: temperature 200°C, holding time 2h, pressure 15MPa, and pressure holding time 10min.
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