Tendon anti-adhesion film prepared from acellular matrix and preparation method thereof

A technology of acellular matrix and anti-adhesion film, applied in medical science, surgery, etc., to promote tendon repair, prevent tissue adhesion, and prolong degradation time

Inactive Publication Date: 2019-07-23
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on tendon anti-adhesion membrane prepared from acellular matrix

Method used

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  • Tendon anti-adhesion film prepared from acellular matrix and preparation method thereof
  • Tendon anti-adhesion film prepared from acellular matrix and preparation method thereof
  • Tendon anti-adhesion film prepared from acellular matrix and preparation method thereof

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Embodiment

[0031] Examples The preparation method and biological performance experimental study of tendon anti-adhesion membrane.

[0032] 1. The specific steps for preparing the anti-adhesion membrane from the bovine Achilles tendon acellular matrix are as follows.

[0033] (1) Take fresh beef Achilles tendon, remove accessory fat, etc., mechanically crush it into small pieces.

[0034] (2) Shake and rinse the crushed animal tissue with deionized water for 5 times.

[0035] (3) Freeze at -80°C for 15 minutes, then bath at 37°C for 20 minutes. Repeat the freezing and thawing process for 5 cycles.

[0036] (4) Shaking treatment with 0.1% sodium lauryl sulfate solution by mass concentration for 6 hours, rinse with deionized water.

[0037] (5) Freeze-dry the obtained extracellular matrix.

[0038] (6) Grind the freeze-dried extracellular matrix with a high-speed homogenizer, dissolve it in a 1mol / L acetic acid solution, and pour it into a flat-bottomed mold at 30°C to dry to form a film.

[0039] (7) ...

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Abstract

The invention relates to the field of biological material, and particularly relates to an acellular matrix tendon anti-adhesion film and a preparation method thereof. The method disclosed by the invention is characterized in that mammalian tendons are used, and an acellular matrix tendon anti-adhesion film is prepared by a process of raw material treatment, decellularization, matrix dissolution, low-temperature drying film formation, neutralization water washing, and the like. The acellular matrix tendon anti-adhesion film provided by the invention eliminates the immunogenicity of heterologousand foreign tissues, retains the active components of the tendon extracellular matrix, directly wraps a tendon injury part, provides a good microenvironment for tendon repair, prevents adhesion of tendons to surrounding tissues, and promotes tendon repair. In addition, the method for preparing the tendon anti-adhesion film has low cost.

Description

Technical field [0001] The invention belongs to the field of biological materials, and relates to the preparation of tissue matrix materials, in particular to an acellular matrix tendon anti-adhesion membrane and a preparation method thereof. Background technique [0002] Tendon adhesion is a common complication after tendon injury. It is the proliferation and invasion of surrounding tissues during the repair of tendon injury, which seriously affects the functional recovery of patients. The long-term immobilization after tendon repair often causes tendon adhesion. With the development and in-depth research on tendon healing mechanism and tissue engineering, finding and developing effective methods to prevent and treat tendon adhesion has become a hot spot in this field. [0003] The absorbable medical membrane can separate the surgical wound from the surrounding tissues through physical isolation to prevent fibroblast invasion, thereby effectively protecting the wound and preventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/00A61L31/14
CPCA61L31/005A61L31/148
Inventor 敖强陶美含
Owner 中国医科大学
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