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Tissue engineering composite stent for osteochondral repair and preparation method thereof

A tissue engineering and composite scaffold technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of slow integration of the two interfaces, repair failure, instability, etc., and achieve beneficial technical effects, wide application prospects, good biological degradability effect

Inactive Publication Date: 2018-12-28
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of this, the present invention aims at the slow integration of the two interfaces in the existing osteochondral repair process, potential unstable factors such as the instability of the graft in the early stage of implantation in the transplant bed, which may lead to repair failure, and provides a A tissue engineering composite scaffold for osteochondral repair and its preparation method

Method used

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  • Tissue engineering composite stent for osteochondral repair and preparation method thereof
  • Tissue engineering composite stent for osteochondral repair and preparation method thereof

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preparation example Construction

[0025] Its concrete preparation method is:

[0026] Step 1. Add the osteoblast suspension or stem cell suspension to the cell-adhesive porous scaffold material, incubate in a 37°C incubator for 0.5-2h, add the corresponding cell culture medium for 14-28 days, and replace it every other day A culture medium to obtain a cell-porous material complex;

[0027] The cell-adhesive porous scaffold material is a porous scaffold containing collagen, gelatin, fibronectin or cell-adhesive polypeptide; for example, gelatin sponge, hyaluronic acid-gelatin composite porous scaffold, fibronectin-based porous scaffold, and the like.

[0028] Osteoblasts are MC3T3-E1, hFOB1.19, etc.; stem cells are mesenchymal stem cells (MSC), embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC), etc.;

[0029] Step 2, resuspending chondrocytes in a hydrogel macromer solution to obtain a cell suspension; wherein the hydrogel macromer is methacrylated hyaluronic acid, acrylated hyaluronic acid, ...

Embodiment 1

[0035] Step 1: Cut the gelatin porous material (gelatin sponge) into small pieces of 0.5cmx0.5cmx0.5cm, place them in a sterile petri dish, and irradiate with ultraviolet light overnight. MC3T3-E1 cells were trypsinized and resuspended in proliferation medium (DMEM plus 10% serum) at a cell density of 10 7 pieces / ml. Add the cell suspension evenly to the gelatin porous material that has been irradiated with UV rays before, squeeze the sponge with a pipette tip to make the gelatin porous material fully absorb the cell suspension, and put the gelatin porous material containing the cell suspension in a 37°C incubator for 2 hours , and then transferred to a 24-well plate; add osteoinductive medium (DMEM medium plus 10% serum, 100nM dexamethasone, 50ug / mL vitamin C, 10mM β-sodium glycerophosphate) for a total of 14 days, and change it every other day Culture solution to obtain MC3T3-E1-gelatin porous material complex;

[0036] Step 2, resuspending the P1 generation chondrocytes i...

Embodiment 2

[0041] Step 1: Cut the gelatin porous material (gelatin sponge) into small pieces of 0.5cmx0.5cmx0.5cm, place them in a sterile petri dish, and irradiate with ultraviolet light overnight. MC3T3-E1 cells were trypsinized and resuspended in proliferation medium (DMEM plus 10% serum) at a cell density of 10 7pieces / ml. The cell suspension was evenly added to the gelatin porous material that had been irradiated by UV light before, and the gelatin porous material containing the cell suspension was placed in a 37°C incubator for 0.5h, and then transferred to a 24-well plate; osteoinductive medium (DMEM 10% serum, 100nM dexamethasone, 50ug / mL vitamin C, 10mM β-sodium glycerol phosphate) were added to the culture medium for a total of 18 days, and the culture medium was changed every other day to obtain the MC3T3-E1-gelatin porous material complex;

[0042] Step 2, resuspending the P1 generation chondrocytes in a 2% (w / v) methacrylated hyaluronic acid solution containing a photoiniti...

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Abstract

The invention discloses a tissue engineering composite stent for osteochondral repair. The stent is obtained by adding a cell suspension dropwise to a cell-porous material composite and then conducting gelating. The cell-adhesive porous stent is a porous stent containing collagen, gelatin, fibronectin or cell-adhesive polypeptide, and all the materials are commonly used biomedical materials with good biocompatibility and are biodegradable in vivo. Hydrogel macromonomer sodium alginate, hyaluronic acid, agarose and chitosan also have good biocompatibility and biodegradability. Polyethylene glycol diacrylate is a biological material that is bio-inert and can be used in vivo, does not cause blood clotting reaction in vivo and is excreted through the kidneys. The composite stent can be used asa bone-cartilage tissue engineering / repair stent material, and has beneficial technical effects and wide application prospects.

Description

technical field [0001] The invention belongs to the field of macromolecule materials, and in particular relates to a tissue engineering composite support for osteochondral repair and a preparation method thereof. Background technique [0002] Full-thickness articular cartilage damage is often accompanied by subchondral bone sclerosis or cystic degeneration. In the later stage of osteonecrosis, the articular cartilage structure can be destroyed due to the collapse of the articular surface. All of them show simultaneous destruction of osteochondral bone. Currently, it is difficult to treat this kind of cartilage combined with subchondral bone injury, and the effect is not good. The development of tissue engineering in the past ten years has provided feasible measures for osteochondral repair. When simple tissue engineered cartilage is used to repair joint osteochondral defects, the healing interface between the graft body and the transplant bed is the integration of the carti...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/52A61L27/38A61L27/20A61L27/18
CPCA61L27/18A61L27/20A61L27/3817A61L27/3852A61L27/52A61L27/56A61L2430/06C08L5/08C08L5/04C08L5/12C08L71/02
Inventor 范长江赵中溢
Owner QINGDAO UNIV
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