The invention relates to the field of fish survival keeping transportation, in particular to a fish ice temperature water-free survival keeping transportation device. The device comprises a box body, a box cover, an oxygen inflating hole and a fish bed, wherein the box body is in a double-layer structure, the box cover is matched with the box body and is movably connected with the box body, the oxygen inflating hole is positioned at the bottom of the side surface of the box body, and the fish bed is positioned inside the box body and is matched with the box body. The fish ice temperature water-free survival keeping transportation device is characterized in that the fish bed is at least in one layer, the double-layer box body comprises an outer layer box body made of thermal isolation materials and an inner layer box body made of heat insulation materials, the thermal isolation materials are composite materials made of aluminum foils and kraft paper, the heat insulation materials are rock wool, and polystyrene foam plastics are arranged between the outer layer box body and the inner layer box body. The fish ice temperature water-free survival keeping transportation device has the advantages that the structure is simple, convenience and practicability are realized, the survival keeping transportation device is suitable for large-scale industrialized production, the energy consumption is not needed, the cost is reduced, and the survival rate of fish bodies is high.