46results about How to "Meet high demand" patented technology

The invention relates to a preparation method of azilsartan, comprising the following steps of: (1) preparing ethoxybenzimidazole-7-methyl carboxylate; (2) preparing 2-cyan-4'-bromomethyl biphenyl; (3) dissolving the ethoxybenzimidazole-7-methyl carboxylate and the 2-cyan-4'-bromomethyl biphenyl into ethanol; adding potassium carbonate to react to obtain 1-[(2'-cyan diphenyl-4-yl)methyl]-2-ethoxybenzimidazole-7-methyl carboxylate; (4) suspending the 1-[(2'-cyan diphenyl-4-yl)methyl]-2-ethoxybenzimidazole-7-methyl carboxylate in water; adding hydroxylamine hydrochloride, sodium hydroxide and tetrabutylammonium fluoride; heating and reflowing, and then cooling; adding the sodium hydroxide and ethyl chloroformate, heating and reflowing to obtain azilsartan methyl ester; and (5) hydrolyzing the azilsartan methyl ester to obtain a product. The preparation method of the azilsartan, disclosed by the invention, has the advantages of being short in process route, high in yield, and safe and reliable; and the purity of the azilsartan obtained by using the method is high.

The invention discloses a metal flange coated with an insulating ceramic coating and a preparation method thereof. The insulating ceramic coating comprises the following material components in percentage by weight: 0 to 50 percent of TiO2 and the balance of Al2O3, wherein the content of impurities is less than or equal to 2.0 percent. The coating position comprises all outer surfaces of a metal flange substrate. The coating has a thickness between 0.1and 0.6mm, a surface roughness less than or equal to 3.2mu m and a porosity less than 15 percent. The preparation method comprises the steps of preparing the metal flange substrate, preparing the coating and processing the coating. In the metal flange coated with the insulating ceramic coating and the preparation method thereof, dissimilar metal flanges are in a reliable electrical insulation state, electrochemical corrosion is prevented, a working temperature of the insulating flange is improved from about 300 DEG C permitted in the prior art to nearly 900 DEG C, the defects of the conventional flange insulation technology can be overcome, and the requirements of petroleum, chemical industry, electric power, ships and the like on the insulating metal flange are met.

The invention discloses a bathroom composite hot water supply system. The bathroom composite hot water supply system comprises a solar heat collection system, a solar heat collection water tank, a heat pump heating water tank, a water source heat pump, a heat pump and an emergency heating water tank, wherein the solar heat collection water tank is connected with the solar heat collection system, the heat pump heating water tank and the water source heat pump are connected with the solar heat collection water tank, and the heat pump and the emergency heating water tank are connected with the heat pump heating water tank respectively. The emergency heating water tank is connected with more than one air energy heat pump and connected to a shower pipe in a bathroom through a pipeline. Both the solar heat collection water tank and the water source heat pump are connected with a waste heat recovery box. A control valve is installed on a cold water inlet of the waste heat recovery box. By the adoption of the bathroom composite hot water supply system, the solar heat collection system and air energy are combined for use, so that the problem in the status quo that energy is insufficient is solved. The system further has the effect of emergency heating, so that the system can be used in special cases. The system has the advantages of saving energy, having environment friendliness significance, meeting current low-carbon life requirements, achieving the goal of continuously and abundantly supplying hot water, meeting heavy demands for hot water, and achieving recovering and reusing heat energy in waste water.

The invention relates to a method for preparing a tissue engineering human corneal stroma carrier bracket from seawater fish collagen. The method comprises the following steps: completely dissolving non-enzymolysis codfish skin collagen freeze-dried powder into hydrochloric acid; centrifuging and removing precipitate; filling supernate into culture board pores respectively and freeze-drying; adding a 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide / N-hydroxysuccinimide crosslinking agent containing chondroitin sulfate and crosslinking; stabilizing the crosslinking effect by using an alkaline solution of Na2HPO4; cleaning by using 2M of NaCl, 1M of NaCl and distilled water sequentially; and freeze-drying to obtain the tissue engineering human corneal stroma carrier bracket. The process is scientific and reasonable; the prepared carrier bracket can be applied to mass production; heavy demand on the carrier brackets due to large-scale reconstruction of tissue engineering human corneal stromata can be met; conditions are created for sight recovery of blind people suffering from human corneal stroma diseases through clinical corneal transplantation; and the preparation cost of the carrier bracket and the application cost of the carrier bracket to in vitro reconstruction of the tissue engineering human corneal stroma and clinical treatment are low.

The invention refers to a balsamroot reproducing method by seeds. It largely raises the reproducing speed by reproducing seeds, meeting the large seedling demands in the production practice and the research. It can produce huge ecological, economic and social benefit.

The invention discloses a method for finishing leather imitating a calfskin style. The method comprises the steps of primary embossing, polishing, dye water spraying, bottom sealing agent spraying, bottom slurry spraying, secondary embossing, surface slurry spraying, three-time embossing, bottom-layer brightening liquid spraying, primary ironing, four-time embossing, top-layer brightening liquid spraying and secondary ironing which are implemented in sequence. The leather with the calfskin style is prepared by using a cow leather billet with a large size and a rough grain surface through a finishing process, and the prepared leather is bright and transparent in appearance, dark in color, fine in grain surface, clear in pores, soft and smooth in hand feeling, plump in body and bone, wear-resistant in coating and high in strength; the preparation method is simple in finishing process and low in cost; the difficulties of purchasing calfskin billets are solved, and a large amount of market demands for the calfskin leather are met. The method disclosed by the invention is suitable for manufacturing the leather with the calfskin style.

The invention relates to a small-DNA-molecular-weight standard, and a standard plasmid and a preparation method thereof. Small DNA sequences with the length of 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp and the like are constructed onto a plasmid by using overlapping polymerase chain reaction, restriction endonuclease digestion, DNA connection and other methods. After the plasmid is completely digested by single restriction enzyme, and seven uniform-brightness strips can be obtained through enzyme digestion. The small-DNA-molecular-weight standard is used for preparing a DNA standard reference substance, and is used for indicating the relative value of DNA molecular weight in agarose electrophoresis.

The invention relates to a solid instant tea containing Qimen black tea, which comprise the Qimen black tea, kudzuvine root, dried longan pulp, spine date seeds and bamboo leaves, wherein the weight percent of the Qimen black tea in the solid instant tea is 35-65%, the weight percent of the kudzuvine root in the solid instant tea is 14-25%, the weight percent of the dried longan pulp in the solid instant tea is 8-15%, the weight percent of the spine date seeds in the solid instant tea is 8-15%, and the weight percent of the bamboo leaves in the solid instant tea is 5-10%. The invention further provides a preparation method of the solid instant tea. The solid instant tea mainly has the functions of regulating the center of five Zang-organs and six Fu-organs and the small intestine, and pesticide residues, heavy metals and other harm substances in tea leaves and herbal plants are effectively removed during the production process, thereby being more in line with higher requirements of modern people on characteristics of greenness, environment friendliness, convenience, quickness, nutrition, health, fashion and the like.

The invention provides an industrial method for improving applicability of senior tobacco leaves through module group secondary rebaking. Senior stock tobacco leaves are subjected to function module grouping, grouping adopts a Schmuck value as a main reference condition, the tobacco leaves with a Schmuck value of 2.8-3.6 are A module Schmuck tobacco leaves with high applicability, the tobacco leaves with a Schmuck value of 2.3-2.8 are B module Schmuck tobacco leaves with medium applicability, and the tobacco leaves with a Schmuck value of 2.3 or below are C module Schmuck tobacco leaves with low applicability; through the grouped function modules, the tobacco leaves are subjected to feed preparation according to a tobacco leaf formula, and are sent to a leaf storage cabinet through vacuum wetting and primary conditioning till the leaf water content is 15%-18% and through secondary conditioning till the leaf water content is 17%-20%, the tobacco leaves in the leaf storage cabinet are subjected top leaf secondary rebaking, a second area temperature key in rebaking is controlled at 70-88 DEG C, temperatures in a first area, a third area and a fourth area are matched and adjusted according to the temperature in the second area, rebaking is completed, and the tobacco leaves are packed in boxes for standby use. According to the industrial method for improving the applicability of the senior tobacco leaves through module group secondary rebaking, the senior stock tobacco leaves can be massively utilized, the stock and the raw material cost are saved, and the tobacco quality is not lowered when the method is applied to a tobacco formula.

The invention discloses a method for cutting propagation by utilizing blueberry tissue culture seedlings. The method disclosed by the invention utilizes the blueberry tissue culture seedlings as cuttings for cutting propagation, combines a rooting culture stage of subculture sprouts in a bottle with transplanting domestication of rooting seedlings, reduces a tissue culture step, saves production costs and greatly shortens rooting time, thereby shortening a seedling raising cycle; and the method explores conditions affecting rooting of cutting of the blueberry tissue culture seedlings through researching influences of different conditions on the rooting of the cutting of the blueberry tissue culture seedlings, thereby improving a rooting rate by utilizing artificial regulation and control.The invention establishes a novel method capable of rapidly reproducing the blueberry seedlings in quantity, and the method provided by the invention can meet heavy demand for blueberries by markets.

The invention provides a cold stamping film formula with high cold stamping firmness and a preparation process thereof, and relates to the technical field of cold stamping printing. The cold stampingfilm formula with high cold stamping firmness comprises a release layer, a laser layer and a gum layer laminated in sequence; the release layer comprises water-based cellulose resins, water, normal propyl alcohol, isopropanol and anhydrous ethanol; the laser layer comprises acrylic resins, chlorine vinegar resins, butanone, butyl ester, ethyl ester, defoaming agents and flattening agents; and thegum layer comprises polyurethane resins, ethyl ester, butanone, silicon powder, defoaming agents and flatting agents. The preparation method of the cold stamping film with high cold stamping firmnesscomprises the following steps: raw materials are mixed to obtain the release layer; release coating liquid is coated on the surface of a PET substrate for drying through a coating machine; the raw materials are mixed to obtain the laser layer; color layer coating liquid is coated on the surface of the release layer for drying through the coating machine; the raw materials are mixed to obtain the gum layer; and gum coating liquid is coated on the surface of a color layer for drying through the coating machine to obtain the cold stamping film. The cold stamping film is stamped to achieve good flatness, high cold stamping firmness and good ink adhesion.

The invention discloses a solid instant tea containing Longjing tea, which comprises the following components in percentage by weight: 35-65% of Longjing tea, 14-25% of cassia seed, 8-15% of pawpaw, 8-15% of wolfberry fruit and 5-10% of chrysanthemum. The invention also provides a method for preparing the solid instant tea. The solid instant tea provided by the invention has the function of regulating the liver in internal organs of a human body during the production process, harmful substances such as pesticide residue, heavy metals and the like in tea leaves and herbaceous plants are effectively removed, and therefore, the higher requirements of modern people on the characteristics such as environmental protection, convenience and quickness, nutrition and health, fashion and the like of drink products are fitted.

The invention discloses water and fertilizer integration special fertilizer for grapes. The water and fertilizer integration special fertilizer for grapes comprises drip irrigation fertilizer, fertilizer applied along with water and spraying fertilizer. The drip irrigation fertilizer is mainly made of urea, ammonium nitrate and potassium chloride. The fertilizer applied along with water is mainly made of monopotassium phosphate, potassium nitrate and phosphoric acid. The spraying fertilizer is mainly made of medium trace element compounds. The invention further provides an application method of the special fertilizer. Compared with the prior art, the obtained water and fertilizer integration special fertilizer for grapes is convenient to use, interaction between the kinds of fertilizer or pipeline blockage caused by the fertilizer can be effectively avoided, besides, nutritional requirements of grapes at different stages can be met just by adjusting the dosage of the kinds of fertilizer, and the cost is further lowered on the basis of the prior art.

The invention discloses a scutellaria baicalensis cultivation technology, and particularly relates to a cultivation method for qualified scutellaria baicalensis planted in the north and transplanted to the south. The cultivation method mainly comprises the steps of land selection and furrowing preparation before seed sowing, land selection and furrowing preparation before seed sowing, seed sowing in the first ten-day period of April, seedling management, transplanting and field planting, field management and harvesting. The seedling time of the scutellaria baicalensis is April in the current year, and the harvesting time is April in the next year. The growth cycle of the scutellaria baicalensis is half a year shorter than the two-year growth cycle of scutellaria baicalensis planted in the origin province of Gansu, so that the scutellaria baicalensis supply time is shortened, and the scutellaria baicalensis production quantity is increased. In addition, due to the ridge cultivation technology and the proper temperature (the mean monthly temperature of the Guangdong area in winter is about 14.8 DEG C and the humidity of the Guangdong area in winter is about 71.6), growth and extension of main roots of the scutellaria baicalensis are ensured, the yield of the scutellaria baicalensis is improved (by 10% to 20%), and according to the content determination, the baicalin content of the scutellaria baicalensis planted in Guangdong is obviously higher than that of the scutellaria baicalensis planted in Gansu. The finger-print reflects that the planted scutellaria baicalensis is not greatly different from the scutellaria baicalensis produced in Gansu, and the heavy market requirement can be met.

The invention relates to a method for preparing polymer monomer 3,4-ethylenedioxythiophene. The method includes firstly dissolving ethyl chloroacetate and sodium sulphide in acetone to react to obtain diethyl thioglycolate; secondly, dissolving the diethyl thioglycolate to ethanol, adding sodium ethoxide and diethyl oxalate into the ethanol, heating and reflowing to obtain ethanol solution of 2,5-dioctyl phthalate ethyl ester-3,4-thiophene glycol sodium; thirdly, adding dichloroethane and tetrabutylammonium bromide into the ethanol solution of the 2,5-dioctyl phthalate ethyl ester-3,4-thiophene glycol sodium, heating and reflowing to obtain 3,4-ethylenedioxy-2,5-dioctyl phthalate ethyl ester thiophene; fourthly, dissolving the 3,4-ethylenedioxy-2,5-dioctyl phthalate ethyl ester thiophene in mixed solvent, adding sodium chloride, heating and reacting, and finally reducing pressure and rectifying. The method for preparing polymer monomer 3,4-ethylenedioxythiophene is few in procedures, low in cost, safe and reliable in technique, simple and fast in post-treatment method, and products are high in yield and purity.

The invention provides a process for obtaining a high-purity adeno-associated virus vector. The process comprises the following steps: A) collecting an adeno-associated virus vector for culturing a supernatant, filtering to remove cell debris, and using a clarified sample for chromatographic purification; B) purifying by a cation column; C) purifying by an adsorption gel filtration column; D) purifying by an anion exchange column; and E) concentrating by an ultrafiltration membrane. In the process provided by the invention, purification is carried out from cell culture of the supernatant, so that a harvesting process of the adeno-associated virus vector is greatly simplified; the process does not involve difficult-to-amplify unit operation such as cracking, centrifugation, precipitation and ultracentrifugation, thereby having great significance for large-scale production and promotion of clinical application of adeno-associated viruses.

The invention discloses baikhovi tea-containing solid instant tea. The baikhovi tea-containing solid instant tea consists of the following ingredients in percentage by weight: 35 to 65 percent of the baikhovi tea, 14 to 25 percent of yam, 8 to 15 percent of grosvener siraitia, 8 to 15 percent of white hyacinth bean and 5 to 10 percent of mulberry leaf. The invention also provides a preparation method of the baikhovi tea-containing solid instant tea. The baikhovi tea-containing solid instant tea has the function of adjusting lung and largegut in internal organs of a human body. In the production process of the baikhovi tea-containing solid instant tea, harmful substances such as pesticide residues, heavy metals and the like are effectively removed from the tea and herbaceous plants; and higher requirements, such as the characteristics of environmental friendliness, convenience, instantness, trophism, healthiness, fashionableness and the like, of modern people on beverage products are met.

The invention relates to a lamp dot matrix control device and method and an LED stage lamp. The lamp dot matrix control device comprises a processing module, a filling module, a triggering module anda direct memory access module. The processing module is used for receiving channel function data sent by a light console and calculating each PWM output value according to the channel function data; the channel function data comprises a lamp channel function and a channel value; the filling module is electrically connected with the processing module and is used for converting the PWM output valueinto binary data and storing the binary data in a memory to form a memory array in the memory; the triggering module is used for periodically generating trigger signals; and the direct memory access module is electrically connected with the triggering module and is used for receiving a trigger signal and transmitting the memory array to an external GPIO interface according to the triggering signal, so that a single MCU microprocessor can output a plurality of PWM signal circuits, and meanwhile, the use quantity and the production cost of the MCU microprocessor are reduced.

The invention relates to a bamboo-stump cultivation method for wild Ganoderma lucidum. Ganoderma lucidum cultivation is carried out through carrying out culture medium preparation, carrying out mycelium culture, carrying out environment setting, carrying out Ganoderma lucidum fruiting body cultivation and carrying out spore powder collection. The method has the beneficial effects that the Ganoderma lucidum cultivation is carried out through selecting bamboo stumps, remaining after bamboos in wild bamboo groves are hewn, as carriers, wild natural-environment cultivation is achieved, and Ganoderma lucidum absorbs wild natural nutritional ingredients and is abundant in nutritional ingredient content, so that the formation state of effective ingredients of Ganoderma lucidum bodies is good, and cultivated Ganoderma lucidum can have the effects of wild Ganoderma lucidum; the medicinal value is high, and the cultivation process is simple, so that the problems of the artificially-cultivated Ganoderma lucidum at present that the medicinal value is low and the cultivation process is complicated are solved, and the yield of Ganoderma lucidum can also be increased; and spore powder can be extensively collected, so that the income of Ganoderma lucidum cultivation is increased, the economic benefit is increased, the heavy demand of markets are met, and the popularization is facilitated.

The invention provides a coriolus versicolor cultivation method. Coriolus versicolor is cultivated through the steps of stock culture, spawn culture, coriolus versicolor cultivation, mycelium culture and coriolus versicolor sporocarp culture. The coriolus versicolor cultivation method has the advantages that wild coriolus versicolor mycelia are taken from field dead wood as strains, on the one hand, the cultivation speed of coriolus versicolor can be increased, the cultivation efficiency of coriolus versicolor can be improved, the cultivation periods of coriolus versicolor can be increased, and on the other hand, cultivated coriolus versicolor can achieve the effect of wild coriolus versicolor; in the steps, the field natural growing environment of coriolus versicolor can be simulated, formation of effective ingredients of coriolus versicolor is facilitated, cultivated coriolus versicolor achieves the effect of wild coriolus versicolor, the yield is increased, heavy demands of the market can be met, the income brought by coriolus versicolor cultivation is increased, economic benefits are increased, and popularization is facilitated.

The invention discloses a planting method for improving yield of radix sophorae flavescentis, and belongs to the technical field of planting of Chinese herbal medicine. Planting of radix sophorae flavescentis is implemented by four steps of: 1, selection and preparation of land; 2, selection and processing of seeds; 3, sowing; and 4, field management. According to the planting method disclosed by the invention, crop yield of radix sophorae flavescentis can be effectively improved, investment cost is low, yield of crops per mu is 2,000 to 2,100kg per mu, yield is increased by 33.3% to 40%, economic benefits are obviously improved, the planting method can be popularized in a large area, and heavy demands of people for radix sophorae flavescentis are met.

The invention relates to a system for synchronously realizing RFID label integrated testing and program downloading in an RFID label card automation production. The system comprises a host computer, a downloading apparatus and an electronic label detection apparatus. The downloading apparatus comprises a downloading main circuit provided with a multipath downloading interface and a chip circuit board provided with a plurality of program downloading holes. The electronic label detection apparatus is connected with the downloading main circuit. The invention also relates to a method for synchronously realizing the RFID label integrated testing and the program downloading in the RFID label card automation production. By applying the system and method provided by the invention, the chip program downloading and the electronic label integrated testing are simultaneously carried out, the production efficiency is enhanced, the practicality and the safety are improved, the weakness of double-frequency active label card automation production is overcome, the production cost is reduced, and the quality requirement is ensured. Therefore, the system and the method have a wider application scope.

The invention relates to the technical field of efficient crop cultivation, in particular to a light, simple and efficient millet cultivation technology. The technology comprises the steps that 1, deep tillage and immediate raking and furrow collection are performed in the soil preparation process; 2, balanced fertilization is performed, and equal stress is laid on base fertilizer, seed fertilizer and additional fertilizer; 3, during seed sowing, seed grains and bean pulp are mixed and sown; 4, water and fertilizer planning and management are performed in the elongation-heading stage; 5, mechanical harvesting is performed. The technology has the advantages that the depth of the deep tillage soil is 25-35 cm, the basement soil permeability is improved, root growth is facilitated, drainage is facilitated, the emergence rate can be improved, and seedlings are strengthened; farmyard manure and phosphorus potassium fertilizer are matched to be used as a base fertilizer, and the fertilizer efficiency is improved; the seed grains and the bean pulp are sown at the same time, seedling thinning can be achieved, and the labor cost is effectively lowered; nitrogen fertilizer is postponed, dressing is performed in the elongation-heading stage, water and fertilizer are planned and managed, kernels per spike are increased, and increment is facilitated; timely harvesting is achieved, mechanical operation is achieved, the labor cost is lowered, and millet planting income of a farmer is increased. The novel technology capable of saving the cost and improving the effect saves labor and force, and is easy and convenient to implement and efficient.

The invention discloses a touch screen display. The touch screen display comprises a base support, the upper end of the base support is provided with a switch, the upper end of the switch is provided with a touch display screen, the touch display screen comprises a liquid crystal panel layer, a transparent insulation layer, a transparent conductive layer and a touch line layer which are successively attached, the periphery of the touch display screen is provided with a housing, and the touch display screen is externally provided with an anti-reflection protection film. According to the invention, under the condition that the requirement for the same appearance and structure is met, the cost is lower, the production power is higher, and the touch screen display can meet vast demands of low and medium-end consumers and has an anti-reflection function.

The present invention provides a 2, 5-bis (benzoxazole-2-) furan preparation method, which is characterized in that furan-2, 5-dicarboxylic acid and ortho-aminophenol are subjected to a condensation reaction in a solvent under the action of a catalyst, the solvent is 1-methylnaphthalene, and the catalyst is boric acid. When the method provided by the invention is used for producing the 2, 5-bis (benzoxazole-2-) furan, the yield of the 2, 5-bis (benzoxazole-2-) furan can reach 95% or above, and industrial mass production can be realized. Therefore, the method provided by the invention can meet the large demand of the market on the 2, 5-bis (benzoxazole-2-) furan serving as a degradable fluorescent whitening agent.

The invention provides a whole-stack hoisting device and method for aerated bricks. The whole-stack hoisting device for the aerated bricks comprises a supporting base and a steel bar sleeve cage withupper and lower openings in a cage structure, wherein the steel bar sleeve cage comprises an upper end hoisting assembly, a lower end supporting assembly and a plurality of vertically arranged steel bar vertical columns, the upper end hoisting assembly comprises four hoisting cross rods enclosing a rectangular frame, the lower end supporting assembly is parallel to the upper end hoisting assemblyand correspondingly arranged, the lower end supporting assembly comprises four supporting cross rods enclosing a rectangular frame, and the steel bar vertical columns are respectively connected with the hoisting cross rods and the supporting cross rods; and the supporting base is detachably connected with the steel bar sleeve cage. According to whole-stack hoisting device and method for the aerated bricks, the device can be manufactured by processing steel bars on a construction site, the structure is simple, the manufacturing time is short, the device is not limited by the environment of theconstruction site, the device is far lower than a bucket and a caterpillar transport vehicle in overall manufacturing cost, the whole-stack hoisting of the aerated bricks is facilitated, and the replacement of the specification of the steel bar sleeve cage according to hoisting amount requirements is facilitated at the same time.

The invention provides a method for producing container-packed aloe leaf pulp. By the method, container-packed aloe leaf pulp containing large amount of aloe leaf pulp can be easily produced within a short time. The method comprises, mixing aloe leaf pulp with syrup to prepare syrup-kept aloe leaf pulp; stirring the syrup-kept aloe leaf pulp while uniformly heating the aloe leaf pulp at a temperature of 88 to 100 DEG C for 3 to 10 minutes for sterilization; packing all the components of the syrup-kept aloe leaf pulp into a container, and cooling; wherein, the heating and cooling are performed to obtain an area, which is enclosed by the a temperature time curve and a time line when the temperature of the syrup is 70 DEG C, of 8-43 (DEG C.hr), the temperature time curve representing the variation of the temperature (DEG C) of the syrup with the time when the syrup-kept aloe leaf pulp is subjected to heating and cooling.

The invention relates to a simple method for preparing liquid needle mushroom spawns, which is characterized in that a sterile operation method is adopted to inoculate prepared mycelium suspension into a triangular flask in which sterilized liquid spawn culture medium is contained, and then the triangular flask is placed on a magnetic stirrer in a constant-temperature incubator for constant-temperature incubation to form yellow small fungus balls, i.e. the liquid spawns. By using conventional microorganism culture equipment for preparing the liquid needle mushroom spawns, the mass production of the liquid needle mushroom spawns can be conveniently conducted by mushroom farmers; and by using the liquid spawns to substitute for solid spawns to realize the culture of needle mushroom fruit bodies, the spawn running time can be shortened, the production cost can be reduced, the production efficiency can be improved and the great market demand can be satisfied.