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Method for cutting propagation by utilizing blueberry tissue culture seedlings

A technology of cutting propagation and tissue culture seedlings, which is applied in vegetative propagation, horticultural methods, botanical equipment and methods, etc., can solve the problems of low rooting rate, achieve the effects of long time, saving production costs, and mass reproduction

Active Publication Date: 2018-07-24
黑龙江朴厚农林科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with tissue culture, cutting propagation is widely used in plant seedling breeding because of its simplicity, short seedling cultivation cycle, and fast rooting speed. However, conventional cutting propagation methods are limited by the number of cuttings and the time for obtaining materials. The low rooting rate of blueberry direct cuttings is the bottleneck restricting the development of clonal production using blueberry cuttings

Method used

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  • Method for cutting propagation by utilizing blueberry tissue culture seedlings
  • Method for cutting propagation by utilizing blueberry tissue culture seedlings
  • Method for cutting propagation by utilizing blueberry tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The establishment of embodiment 1 blueberry tissue culture seedling cutting propagation method

[0034] 1 Materials and methods

[0035] 1.1 Cultivation of blueberry tissue culture seedlings

[0036] 1.1.1 Materials and pre-processing

[0037] This experiment was carried out in the laboratory of Horticulture Branch of Heilongjiang Academy of Agricultural Sciences from November 2014 to September 2016. The test materials were collected in the blueberry garden of the Horticultural Branch of Heilongjiang Academy of Agricultural Sciences in November 2014. The blueberry varieties were Berkeley, Meideng and Sunrise.

[0038]Take the 1-year-old branches of the three varieties collected back to the laboratory, promote the germination of axillary buds by hydroponics, cut off when the axillary buds germinate and grow to about 2cm, put them in an ultra-clean workbench, and soak them in 75v / v% alcohol 30s, rinsed with sterile water for 4 times and then transferred to 0.1w / w% HgCl...

Embodiment 2

[0108] Example 2 Research on Morphology and Anatomy in the Rooting Process of Blueberry Cuttings

[0109] Using blueberry Maideng tissue culture seedlings as experimental materials, the changes of external morphology and rooting anatomy during the development of adventitious roots of cuttings were studied.

[0110] 1 Cutting Propagation Method of Blueberry Mayden Tissue Culture Seedlings

[0111] Adopt the best technical scheme that embodiment 1 establishes to carry out, specifically comprise the following steps:

[0112] (1) The 1-year-old branches of blueberry Mayden collected were hydroponically promoted to germinate the axillary buds, cut off when the axillary buds germinated and grew to 2cm, soaked in 75v / v% alcohol for 30s, rinsed with sterile water for 4 times, and then transferred to 0.1 w / w%HgCl 2 solution, disinfected for 8 minutes, and finally rinsed with heavy distilled water for 5 times, put it into the ultra-clean workbench after disinfection, and received it i...

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Abstract

The invention discloses a method for cutting propagation by utilizing blueberry tissue culture seedlings. The method disclosed by the invention utilizes the blueberry tissue culture seedlings as cuttings for cutting propagation, combines a rooting culture stage of subculture sprouts in a bottle with transplanting domestication of rooting seedlings, reduces a tissue culture step, saves production costs and greatly shortens rooting time, thereby shortening a seedling raising cycle; and the method explores conditions affecting rooting of cutting of the blueberry tissue culture seedlings through researching influences of different conditions on the rooting of the cutting of the blueberry tissue culture seedlings, thereby improving a rooting rate by utilizing artificial regulation and control.The invention establishes a novel method capable of rapidly reproducing the blueberry seedlings in quantity, and the method provided by the invention can meet heavy demand for blueberries by markets.

Description

technical field [0001] The invention relates to a method for tissue culture of blueberries, in particular to a method for cutting propagation of blueberry tissue culture seedlings, and belongs to the technical field of agricultural planting. Background technique [0002] Blueberry (blueberry), belongs to Rhododendronaceae (Eri-caceae) Vaccinium (Vaccinium) plant, has another name called lingonberry, blueberry, is a perennial small berry shrub fruit tree. [0003] Blueberry fruit is rich in sugar, acid, protein, vitamins, mineral elements, in addition to anthocyanins, arbutin, niacin, tannic acid, flavonoids, antioxidants, folic acid and other substances. The fruit has health functions and medicinal value such as protecting eyesight, relieving visual fatigue, delaying aging, anti-cancer, enhancing human immunity, and softening blood vessels. In addition, blueberry fruit also has extremely high economic value. In addition to being eaten fresh, the fruit can also be processed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01H4/00A01G24/22A01G24/15C05G3/00
CPCA01H4/001A01H4/008C05D9/00C05D9/02C05G3/00C05G5/23C05F11/00C05F11/02
Inventor 杨瑞华闫绍鹏王柏林鲁会玲覃杨叶万军王禹董畅任爱华刘万达肖丽珍王雪娇
Owner 黑龙江朴厚农林科技有限公司
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