The invention relates to PTC (Positive Temperature Coefficient) heat-conducting ink and a preparation method thereof, and relates to the technical field of heat-conducting ink. The PTC heat-conducting ink comprises conductive particles, a heat-conducting filler A, a coupling agent and a solution, and the mass ratio of the conductive particles to the heat-conducting filler A to the coupling agent to the solution is (1-1.2): (0.5-2): (0.003-0.01): (4.5-6); the conductive particles comprise a conductive filler B, a semi-crystalline polymer and a solvent, the conductive filler B is one or a combination of more of carbon black, graphite, graphene, titanium diboride and metal micro powder, and the solvent is one or a combination of more of xylene, ethyl acetate, N, N-dimethylformamide and ethylene glycol butyl ether acetate; the heat-conducting filler A is one or a combination of more of aluminum oxide, zinc oxide, magnesium oxide, aluminum nitride and boron nitride; the solution comprises a binder and a solvent. The PTC heat-conducting ink disclosed by the invention is low in temperature control, good in heat conductivity, high in PTC ratio, stable in room-temperature resistance and fast in response; and the preparation method is simple to operate and can be suitable for large-scale production.