78results about How to "Fast combination" patented technology

The invention provides a porous dental implant capable of carrying a drug sustained release system. The porous dental implant comprises an implant body. An external thread connected with alveolar bone is arranged on the outer surface of the implant body. Porous structures are formed among adjacent threads of the external thread. Pores used for storing sustained release drugs are formed in the porous structures. The invention provides a preparing method of the porous dental implant capable of carrying the drug sustained release system. According to the porous dental implant capable of carrying the drug sustained release system and the preparing method thereof, bonding strength is effectively improved, bonding speed is increased, and a porous structure can be achieved easily.

The invention relates to the technical field of detection of ischemia modified albumin content, and in particular to a detection kit for ischemia modified albumin. The kit includes a reagent 1 and a reagent 2. The reagent 1 contains a buffer, cobalt chloride, a stabilizer and a preservative; and the reagent 2 contains a buffer, dithiothreitol, a stabilizer, a reducing protective agent and a preservative. The kit can accelerate the binding efficiency of cobalt ions with normal albumin, and the cobalt ions fully integrate with normal albumin in a certain period of time, so as to ensure the accuracy of the detection result on a sample by the reagent; the kit protects the stability of dithiothreitol in the solution, and does not affect the binding of dithiothreitol with cobalt ions, so as to ensure that an open reagent bottle can be stably kept in a dark place for 30 days at room temperature and a closed reagent bottle can be stably kept at 2-8 DEG C for 12 months, and fully meet the needs of clinical laboratory; and the kit accelerates the combination of dithiothreitol with cobalt ions, so that the reagents reach reaction endpoints as soon as possible, thereby ensuring the efficient detection effect of reagents and significantly improving the accuracy of reagent detection.

The invention discloses a preparation method of surface imprinted material for matrine substance separation and purification, which aims to solve the problem of low efficiency of the surface imprinted material for separating matrine, and comprises the following steps that: silica particles are activated; a silica particle surface chemical bond is connected with silane coupling agent containing double bonds; the surface of the silica particles is chemically grafted with polymethylmethacrylate; and silica particle surface molecular imprinted polymer is prepared. The invention prepares the surface imprinted material which has high molecular association speed, strong identification selectivity and high association capacity to the matrine substance with the silica particles as a carrier, the methacrylate as functional macromolecular material to be grafted with the particles or the surface of the particles and the double-bond silane coupling agent as cross linker; and the surface imprinted material prepared through the method is applicable to the requirements of industrial production, has high material utilization rate, low cost, good selectivity to matrine substance molecules, very strong identification and association properties, and very good desorption performance.

The invention relates to methods for preparing and using a sudan red magnetic molecularly imprinted polymer. The methods aim to solve the problems that the conventional separated / purified sudan red I magnetic molecularly imprinted polymer has a 'solution polymerization' phenomenon in the synthesis process and the obtained separated / purified sudan red I magnetic molecularly imprinted polymer has a non-uniform spatial network structure. The preparation method comprises the following steps of: 1, preparation of Fe3O4; 2, preparation of magnetic SiO2; 3, preparation of aminated magnetic SiO2; 4, preparation of initiator / magnetic SiO2; and 5, polymerization reaction. The using method comprises the following steps of: adding the sudan red magnetic molecularly imprinted polymer into a sample solution containing sudan red, and then sequentially performing oscillatory reaction, magnetic separation and elution. The method is mainly used for preparing the sudan red magnetic molecularly imprinted polymer.

The invention discloses a multistage through-hole porous tooth implantation body. The multistage through-hole porous tooth implantation body comprises a gingiva passing through segment and a screw thread segment; the gingiva passing through segment is of a smooth solid structure, and is arranged on the upper part of the screw thread segment; the multistage through-hole porous tooth implantation body also comprises a porous structure segment; the porous structure segment is arranged on the lower part of the screw thread segment; the hole diameter of transverse through holes on the porous structure segment from the lower part to the upper part is increased successively; the transverse through holes with the same hole diameter are arranged in a radiation manner along the periphery of the porous structure segment. The multistage through-hole porous tooth implantation body is capable of increasing combination strength, increasing combination speed, and is provided with the porous structure conveniently.

The invention belongs to the field of the glycoproteomics and glycomics, and relates to a method for rapid enzymolysis release, solid phase concentration and mass spectrometry of N-glycan. The methodcomprises the following steps of absorbing a solution containing a glycoprotein sample by using a degreasing cotton material, and performing high-temperature rapid PNGase F enzymolysis on the cotton to release N-glycan; adjusting solution environment after the enzymolysis is finished, and adsorbing free glycan on the degreasing cotton material by utilizing the high-density hydroxy and hydrogen bond of the glycan and like effects on the surface of the degreasing cotton material, and then cleaning and removing non-glycan molecules such as protein, polypeptide and like which are not bonded with the degreasing cotton material, and finally eluting the adsorbed glycan with the aqueous solution, and feeding into mass spectrometry. Through the method disclosed by the invention, the used material is cheap and easy to obtain, the preparation is easy, the operation is simple and convenient, the high-speed enzymolysis of the N-glycoprotein and the high-selective concentration and high-sensitive mass spectrometry of the N-glycan can be realized.

The invention discloses a preparation method of a new material for separating chiral aspartic acid, and solves the problems of difficulty and low efficiency of L-Asp separation currently. The method comprises the following steps of: activation of silica gel particles; chemical bonding of the silica gel particles with a double bond-containing silane coupling agent; chemical grafting of the silica gel particles with polydimethylaminoethyl methacrylate; and preparation of silica gel particle surface molecularly imprinted polymer. The invention adopts silica gel particles as carriers, adopts dimethylaminoethyl methacrylate as a functional macromolecular material grafted on the particles or the particle surfaces, and adopts ether dihalide as a cross-linking agent to prepare the surface imprinted material with high molecular binding speed, strong recognition selectivity, high binding capacity to L-Asp; the surface imprinted material prepared by the invention is suitable for the requirements of industrial production, and has high material utilization rate, low cost, and very good selectivity, recognition and bonding performance to L-Asp molecules.

The invention relates to a titanium metal osseointegration implant, which comprises an implant body, wherein the ragged coarsening bonding surface is formed on the surface of the implant body through sandblasting, shot blasting, plasma titanium spraying or electrolytic corrosion, and the coarsening bonding surface on the implant body surface forms the clean bonding surface having micro pores through acid liquid corrosion. According to the present invention, after the acid corrosion treatment on the implant, the surface impurities can be removed, the osseointegration strength can be increased, and the infection risk can be reduced. The titanium metal osseointegration implant surface treatment method comprises: 1, forming a ragged coarsening bonding surface on the surface of an implant through sandblasting, shot blasting, plasma titanium spraying or electrolytic corrosion; and 2, carrying out acid corrosion on the implant so as to form the clean coarsening bonding surface on the surface. In addition, the implant is treated so as to form the coarsening bonding surface on the surface, and then the acid corrosion treatment is performed, such that the impurities on the implant surface can be removed, the cleaning degree of the implant can be improved, and the infection risk can be reduced.

The invention discloses a method for labeling protein through quantum dots and belongs to the field of nano-biotechnology. The method is characterized by expressing a Histag on protein, obtaining a protein dimer containing two Histags through protein dimerization, mixing the protein with the quantum dots according to a certain ratio, and oscillating to enable the two Histags to combine with the quantum dots simultaneously so as to improve the stability of combination. The method is simple in operation, and the quantum dots can be applied to biomarker widely as a nano fluorescent probe.

The invention relates to the dental implanting field, in particular to a surface treatment method applied to a titanium or titanium alloy dental implant. The surface treatment method comprises the following steps: performing sand spraying treatment on the titanium or titanium alloy dental implant; and using a phosphoric acid / oxalic acid mixed solution to perform acid etching treatment after sand spraying is accomplished, thereby obtaining the titanium or titanium alloy dental implant with a multistage hole structure. The surface treatment method has the advantages that the process is simple, is safe, is environmentally friendly and is beneficial for reducing cost. The implant treated by the process defines the multistage hole structure comprising 10-50-[mu]m large holes and 2-5-[mu]m smallholes, so that residual particles are greatly reduced (average particle residues smaller than 5 / mm<2>), surface biological activity can be improved, adhesion, on the implant surface, of osteoblast ispromoted, bone combining speed is favorably increased, and success probability of dental implanting is improved.

The invention belongs to the technical field of desulfurization and particularly relates to a high airspeed iron desulfurizing agent, a preparation method and application of the desulfurizing agent. The desulfurizing agent provided by the invention takes activated zinc oxide with nanoparticle small size effect as an additive; the additive is fully utilized; when hydrogen sulfide molecules escape under the condition that a hydroxyl ferric oxide component is combined with hydrogen sulfide till reaching a saturated state, the activated zinc oxide can react with the escaping hydrogen sulfide; a compound active center is formed under the interaction of the hydroxyl ferric oxide and the activated zinc oxide, and the desulfurizing precision is effectively improved; meanwhile, the compound active center also can effectively accelerate the combining of the desulfurizing agent and the hydrogen sulfide molecules and the unit handling capacity of the desulfurizing agent can be increased. The desulfurizing agent provided by the invention can meet the high airspeed desulfurizing requirement; the desulfurizing precision is high; the hydrogen sulfide content in the desulfurized gas is smaller than 0.1ppm; the penetrating sulfur capacity under the airspeed of 6000h-1 under room temperature can reach up to 33%.

The invention discloses a novel annular polypeptide ligand (ATTO-H6) used for modifying quantum dots, and belongs to the technical field of nano-biology. The novel annular polypeptide ligand comprises a dye ATTO 590 (1) used for undergoing FRET together with quantum dots, a sequence (2) used for polypeptide cyclization, histidine intervening sequences (3) used for combining the quantum dots, and sequences (4) used for polypeptide folding, and all sequences are combined through peptide bonds. The ligand is simple to synthesize, and the combination of six histidine intervening sequences and the quantum dots greatly improves the combination rate and stability of the ligand and the quantum dots, so the ligand can be widely used in biological marking as a fluorescence nano-probe.

The invention discloses a pre-film cleaning treatment method of a circulating water system. Organic phosphine is adopted to replace sodium hexametaphosphate as a pre-film agent for cleaning pre-films.The pre-film phase is not influenced by turbidity of water after cleaning and iron ions; the better pre-film effect can be achieved; and a replacement step after cleaning can be saved. Meanwhile, a lot of waste water generated by replacement is reduced; and fresh industrial water and the treatment cost caused by pollution discharge are saved.

The invention discloses a method for marking a protein polymer by using quantum dots and belongs to the field of a nano-biotechnology. The method is characterized by the following steps: selecting a fluorescent protein dimer (TIP1-mCherry dimer) containing a histidine tag sequence (Histag); and mixing protein and the quantum dots according to a certain ratio and coupling to form a protein-quantum dot composite, wherein the structure of the composite is proved to be very stable through imidazole substitution. According to the method, systematic research is performed on the influence of the assembling characteristic of the protein with a three-dimensional structure and the quantum dots, so the combining stability of the protein and the quantum dots is improved and the marked protein polymer can serve as a nanometer fluorescent probe widely applied to a biomarker.

The invention provides preparation and application methods of a carbon-nanotube-base magnetic bactericide molecularly-imprinted polymer, relating to preparation and application methods of bactericide molecularly-imprinted polymers. The invention aims to solve the problems of time consuming, high organic solvent consumption and low selectivity in the sample pretreatment technique in the existing bactericide residue analysis, the problem of the solution polymerization phenomenon in the existing bactericide molecularly-imprinted polymer synthesis process, and the problem that the solution separation process needs the vacuum filtration or centrifuging step. The preparation method comprises the following steps: 1. activating carbon nanotubes; 2. hydroxylating the carbon nanotubes; 3. preparing the magnetic carbon nanotubes; 4. preparing the initiator / magnetic carbon nanotubes; and 5. carrying out polymerization reaction. The application method comprises the following steps: adding the carbon-nanotube-base magnetic bactericide molecularly-imprinted polymer into a bactericide-containing sample solution, and sequentially carrying out oscillation, magnetic resolution and elution.

The invention provides a technology for improving activity of an enzyme-labeled working fluid anti-human IgA alkaline phosphatase. The technology comprises preparing an enzyme-labeled secondary antibody working solution according to the characteristics of anti-human IgA alkaline phosphatase self. The improvement comprises successively adding the following substances into the prepared enzyme-labeled secondary antibody: (1) adding a low-concentration high-molecular polymer, and dissolving the added high-molecular polymer in the prepared enzyme-labeled secondary antibody working solution; and (2) then adding anti-human IgA alkaline phosphatase into the enzyme-labeled secondary antibody working solution added with the high-molecular polymer, and stirring to fully dissolve the anti-human IgA alkaline phosphatase, so as to obtain an enzyme-labeled secondary antibody working solution with activity-improved anti-human IgA alkaline phosphatase. According the method, by adding the low-concentration high-molecular polymer, the effects of improving the reaction activity of anti-human IgA alkaline phosphatase and improving the detection sensitivity are realized.

The invention discloses an ecological bed for pretreating coking wastewater. The ecological bed is cylindrical; the cylindrical ecological bed is provided with an annular water inlet, a first-stage magnetic field generator, a second-stage magnetic field generator and an iron-carbon filler filling the space between the first-stage magnetic field generator and the second-stage magnetic field generator; and a plurality of water inlets are uniformly distributed in the lower part of the first-stage magnetic field generator. Water passes through the iron-carbon filler, then flows across the second-stage magnetic field generator, and is discharged out of the ecological bed from a water outlet, wherein the first-stage magnetic field generator and the second-stage magnetic field generator are connected in series through a wire and then are connected with an external power supply. According to the ecological bed, the magnetic field generators are additionally arranged to two sides of the iron-carbon filler, and after a magnetic field is added to the iron-carbon filler, the iron-carbon filler generates micro-vibration under the action of the alternating magnetic field, so that the situation of material hardening and blocking is effectively avoided. In addition, the magnetic field can enable adhesion of a polymer on a biological film surface on the filler to be better, meanwhile, activityof enzymes is improved, and treatment efficiency of the filler is further improved.

The invention provides a treatment process for a dental implant surface. According to the treatment process for the dental implant surface, the dental implant surface is treated through the steps of pretreatment, sand blasting treatment, acid etching treatment, cleaning, color spraying detection, and secondary cleaning, uniformity of pits in the dental implant surface is improved, the treatment process for the dental implant surface is characterized in that a multi-stage hole structure with biological activity is formed on the surface of a pure titanium implant, so that the biological activityof the pure titanium dental implant is improved, stability of the implant is improved, the combination speed of the dental implant and a bone can be increased, the bonding strength of the implant anda bone interface can be improved, the treatment process for the dental implant surface has the advantages of being simple and efficient in process and low in cost, abrasive paper grinding pretreatment is carried out before the dental implant surface is processed, so that forming uniformity of the multi-stage hole structure is improved.

The invention relates to the technical field of water treatment equipment, particularly to circulating filtration and sedimentation equipment for water treatment. According to the invention, a rotating shaft which is coaxial with a sedimentation barrel is rotatably mounted on the pipe wall of the lower part of a first connecting pipe; the top end of the rotating shaft extends upwards into the first connecting pipe; a turbine is fixedly mounted at the top end of the rotating shaft; the bottom end of the rotating shaft extends downwards into the sedimentation barrel; a stirring shaft is fixedlymounted at the bottom of the rotating shaft; a plurality of stirring blades are fixedly arranged on a shaft body of the stirring shaft; a second connecting pipe communicating with the interior of a filtering barrel is fixedly installed at the bottom end of the filtering barrel; the tail end of the outer side of the second connecting pipe is fixedly connected to a water inlet of a first water pump;a water outlet of the first water pump is connected with one end of the first connecting pipe; and a diversion mechanism is fixedly installed at the other end of the first connecting pipe. With the equipment, water can be filtered multiple times, and sewage is stirred in a water flow driving mode, so more energy is saved; and meanwhile, impurities in the sedimentation barrel can be automaticallytreated, and more convenience is obtained.

The invention discloses aspergillus niger capable of rapidly degrading zearalenone and application of the aspergillus niger, and belongs to the field of microorganisms. According to the invention, a strain of capable of effectively degrading the zearalenone is screened out by performing capable of effectively degrading zearalenone is screened out on original aspergillus niger. The strain has highbinding speed with zearalenone and high degradation efficiency, and can degrade 95% or more of the zearalenone within 28h; a relatively high degradation effect can be achieved by using a relatively small amount of zearalenone; degradation of the same concentration of zearalenone needs 1 / 10 of the use amount of the original strain, and the strain is a food-grade strain; and the aspergillus niger can be used for degrading zearalenone in cereals such as corn and barley.

The invention discloses an organic fertilizer for preventing soil hardening damage and a production method thereof. The organic fertilizer comprises the following raw materials in parts by weight: 10-14% of humus soil and 5-10% of quick lime. 8-12% by weight of turf ash; 6-8% by weight of plant ash; 4-7% by weight of pond sludge; 2-4% by weight of a soil loosening agent; 30-40% by weight of a slow-release nutrient material; 3-6% by weight of a biodegradable resin; 3-6% by weight of modified zeolite spheres; 2-4% by weight of pesticide degrading bacteria; 1-3% by weight of soil probiotics; 0.08-1% by weight of a wedelia chinensis extracting solution; 1-4% by weight of crotalaria sessiliflora; 0.2 to 0.6% by weight of urea; the soil conditioner has a strong advantage combination effect, the comprehensive performance of the product can be greatly improved, the salinity and alkalinity of soil are reduced while the soil fertility is improved, and the physical and chemical properties of the soil are improved.

The invention discloses a preparation method of a new material for separating and purifying chiral tetramisole (TM), which is used for solving the problem of low efficiency of the existing surface imprinted material for separating L-TM. The preparation method comprises the following steps: activating silica gel particles, and connecting the surfaces of the silica gel particles with an amino-containing silane coupling agent through chemical bonds; chemically grafting the surfaces of the silica gel particles with polymethylacrylic acid; and preparing a silica gel particle surface molecular imprinted polymer (SMIP). In the method, the silica gel particles are taken as a carrier, methacrylic acid is taken as a functional macromolecular material grafted with the surfaces of the particles or microspheres, and the amino-containing silane coupling agent is taken as a cross-linking agent to obtain the surface imprinted material which has the advantages of fast binding speed, strong recognitionand selectivity and high binding capacity on L-TM molecules. The surface imprinted material prepared by the method has the advantages of high material utilization ratio, low cost, and good selectivity and recognition and associativity on the L-TM molecules, thus meeting the requirements for industrial production.

The invention discloses a water saving sampling type water source purifying device, which comprises a water tank. A water cavity with an upward opening is arranged in the water tank. The right side ofthe water tank is provided with a base. A measuring box is fixed on the top end of the base. In the measuring box, the top wall is provided with a water inlet with an upward opening; between the water inlet and a water cavity, a siphon is arranged and is communicated with the water inlet and the water cavity; and titration cavities are symmetrically arranged on the left end wall and right end wall of the water inlet. A medicament is used to carry out reactions with ions to generate precipitates so as to measure the concentration of harmful ions in water; through stirring, silts in water and water are mixed to form turbid water, and then light penetration detection is carried out to measure the content of silts in water. The detection range is wide, and the water quality detection effect is good. Furthermore, when the device finds that the water quality is bad, the device can automatically purify the water source, the automation degree is high; the sensitivity is high; at the same time, the ion measurement is accurate, the measurement error is small, the generated precipitates can be uniformly recovered and processed, and the device is green, environmentally friendly, safe, and reliable.

The invention discloses a preparing method for an antibacterial nanofiltration membrane with an ultrafiltration membrane as a base membrane. The preparing method includes the following steps that 1, the ultrafiltration membrane with charge performance is soaked in a poly (allylamine hydrochloride) solution in vacuum, and sodium hydroxide is added dropwise to adjust the pH value of the solution to obtain a polycation composite membrane; 2, the polycation composite membrane is washed with deionized water and then soaked in a polyethylene sulfate solution, and meanwhile an ammonium nitrate solution is added to obtain the supramolecular polymer composite membrane; 3, the supramolecular polymer composite membrane is washed with deionized water and then soaked in an epsilon-polylysine solution. The preparing method of the novel nanofiltration membrane high in separating property and bacterium resistance is high in membrane preparing efficiency, short in membrane preparing time, environmentally friendly and efficiency as the water is used as solvent in the preparation process without organic solvent.

The invention discloses a cultivation method of Morchella esculenta, and the cultivation method includes the following steps: S1. preparing a Morchella esculenta strain: the Morchella esculenta strainis composed of a type A strain and a type B strain, wherein the type A strain includes the following raw materials in percentages by weight (a total of 100%): 65-75% of wheat grains, 20-30% of rice husks, 1-5% of gypsum, 0.2-1% of K2(subscript)PO4(subscript) and 0.2-1% of MgSO4(subscript); and the type B strain includes the following raw materials in percentages by weight (a total of 100%): 82-88% of cotton seed hulls, 5-10% of three powder, 0.5-2% of brown sugar, 0.5-2% of gypsum, 1.5-4% of quicklime, 0.2-1% of K2(subscript)PO4(subscript) and 0.2-1% of MgSO4(subscript); S2, sowing the type Astrain, spraying special strain nutrient solution for the Morchella esculenta, and carrying out soil covering; S3, sowing the type B strain on a ridge surface after soil covering, carrying out mulchfilm covering, and then carrying out a nutrition bag treatment; and S4, carrying out mushroom inducing and cultivating treatments, thereby obtaining the Morchella esculenta. The cultivation method ofthe Morchella esculenta provided by the invention solves technical problems of high and stable yield of artificial cultivation of the Morchella esculenta by optimizing the composition formula and thesowing mode of the Morchella esculenta, and makes the nutritional value and the flavor of the cultivated Morchella esculenta similar to wild Morchella esculenta at the same time.

The invention discloses a clutch. The clutch comprises a pressing plate, a friction plate assembly and a central sleeve. The pressing plate is provided with a positioning guide part in radial positioning and axial guide fit with the central sleeve near an external spline of the central sleeve; and the pressing plate is meshed with the central sleeve through a bevel meshing pair. According to the clutch, the centering precision of the clutch is improved, vibration generated when the clutch is combined and separated is reduced, and the control stability of the clutch is guaranteed; meanwhile, the central sleeve and the pressing plate are associated through the bevel meshing pair, and when set relative rotation occurs, the bevel meshing pair can increase the output torque of the clutch and increase the reserve coefficient of the clutch, so that the force transmission capacity is improved, the sliding abrasion time of the clutch is shortened, and fault ablation is avoided; and a positioning guide mechanism can enable the alignment of the bevel meshing pair, meshing and pressing processes are smooth, and vibration, clamping stagnation and ablation of the clutch in the jointing process can be helpfully avoided, so that the service life of the clutch can be helpfully prolonged, and the overall performance of the clutch can be improved.

The invention relates to an organic fertilizer for ameliorating salinity and alkalinity of soil and eliminating soil hardening and a preparation method of the fertilizer. The organic fertilizer for ameliorating salinity of the soil and alkalinity and eliminating soil hardening provided by the invention reduces salinity and alkalinity of the soil while improving fertility of the soil, improves physical and chemical properties of the soil, increases a granular structure of the soil, increases permeability of the soil, improves water retention, fertilizer retention and gas permeability of the soil, greatly increases available nutrients that can be directly absorbed by plants in the soil, eliminates soil hardening, improves resistance to insects and stress of the plants, significantly improvescomprehensive productive capacity of cultivated land, and can be widely used in the technical field of agricultural fertilizers.

The invention relates to a preparation method of a nano composite membrane taking polyvinyl alcohol (PVA) doped with a two-dimensional nano material as a modification layer, and belongs to the technical field of membrane preparation. An ultrafiltration membrane or a microfiltration membrane is used as a base membrane, PVA doped with a two-dimensional nano-material is used for modifying the base membrane, the binding force of the two-dimensional nano material and the base membrane is improved by utilizing the binding performance of PVA, the problem that the nano-material in the composite membrane doped with the nano material is easy to fall off is solved, and a cross-linking agent is used for cross-linking; the stability of the nano material is further enhanced, PVA is prevented from swelling and dissolving, then interfacial polymerization is carried out on the modified base membrane, and the compact and ultrathin separation layer is prepared. The modification layer prepared on the surface of the base membrane regulates the surface appearance of the base membrane, better regulates the interfacial polymerization reaction process and the skin layer structure of the membrane, and greatly improves the rejection rate and flux of the composite membrane.

The invention relates to the technical field of immunoassay detection, and particularly discloses an antigen-antibody detection method which comprises the following steps: a characteristic response frequency band determination step: determining a characteristic response frequency band F1 of a chip of a preset type through different background solutions, and determining a characteristic response frequency band F2 caused by a sample through a sample solution; a selection step: judging whether the characteristic response frequency band F1 and the characteristic response frequency band F2 coincide or not; and a detection step: if coincidence exists, adding a sample on the chip, selecting a specific frequency band based on coincidence under a specific voltage, carrying out measurement under an acceleration condition, and determining the content of a target object in the sample or carrying out qualitative judgment according to the change rate of an electric signal value obtained by measurement. By adopting the method provided by the invention, the universality of chip immunodetection can be improved.