111results about How to "Facilitate enzymatic reactions" patented technology

The invention relates to instant plant total-nutrient food replacement powder which comprises the following raw materials in parts by weight: 10-98 parts of grains, fruits and vegetables, and nut powder, and 0-10 parts of a natural sweetening agent. According to the instant plant total-nutrient food replacement powder, the grains, the fruits and the vegetables, and nuts are mixed and blended, treated by virtue of microwaves, subjected to enzymolysis, and once extruded and cooked, and crushed to prepare the instant plant total-nutrient food replacement powder. The instant plant total-nutrient food replacement powder is complete in nutrients, convenient to prepare, and convenient to digest and absorb, and has the advantages of that the processing period is greatly shortened, the energy consumption is reduced, the cost is lowered, and the storage is easy since microorganisms are killed.

The invention discloses a soybean peptide for promoting lactobacillus proliferation and improving lactobacillus survivability as well as a preparation method and an application thereof, and belongs to the field of deep processing of soybean proteins. The preparation method comprises the following steps: extruding soybean protein at high temperature; damaging a tertiary structure for maintaining the protein through shearing and friction dual functions, thereby enabling the hydrophobic group of the soybean protein to spread; cutting the denatured protein into small molecule peptides by utilizing protease; and performing centrifugal separation, filtering, drying and the like to obtain the soybean peptide. The yield of the soybean peptide reaches up to 72%; and the soybean peptide is low in production cost and can be used for effectively promoting the proliferation of lactobacillus, shortening the fermentation time and improving the survivability of the lactobacillus in a storage period. In addition, the soybean peptide can be applied to fermented dairy products or other foods. Therefore, the soybean peptide is a health food ingredient with high quality.

The invention discloses a method for industrially producing ethyl alcohol through crop straw. The method includes the following steps of pulverizing the crop straw, adding the pulverized crop straw and water into a preprocessing reactor in proportion to be preprocessed, guiding the materials into an enzymolysis tank, conducting cooling, adjusting the pH, adding an enzyme preparation for enzymolysis, guiding the materials into a fermentation tank, adding yeast for ethyl alcohol fermentation production, guiding the materials into a distilling device to be distilled to obtain ethyl alcohol and waste slag liquid, inoculating high-temperature activated sludge to the waste slag liquid for anaerobic fermentation to obtain biogas and digestive slag liquid, making the biogas combusted to generate vapor or used as a production raw material and supplied outward to an urban gas pipe network, squeezing the digestive slag liquid to obtain clear liquid and solid slag, discharging the clear liquid after the clear liquid is processed to reach the standard, combusting the solid slag to generate vapor, and reusing the vapor in the steps of the method. The whole method is advanced in process, high inethyl alcohol yield, sufficient in raw material utilization, low in cost and suitable for industrial production.

The invention discloses a preparation process of almond blood pressure reducing functional oligopeptides. The process comprises the following steps of (1) performing pretreatment: drying and smashing almond meal, performing milling a colloid mill, then, performing freezing and microwave thawing, and next performing leaching centrifugation to obtain protein precipitation; (2) performing complex enzyme ultrasound enzymolysis: performing ultrasonic treatment at 50KHZ and 200W by complex enzyme, and performing an enzymatic hydrolysis reaction for 1 to 2 hours; (3) performing high-temperature enzyme deactivation; (4) performing centrifugal separation to obtain almond peptide raw liquid; (5) adding active carbon powder accounting for 1 to 3 percent of the weight of the almond peptide raw liquid, and performing decoloration and debittering treatment to obtain almond peptide fine filter liquid; and (6) performing vacuum concentration and spraying drying on the almond peptide fine filter liquid to obtain finished product almond oligopeptides. The preparation process of the almond blood pressure reducing functional oligopeptides has the advantages that the almond meal is used as the raw material; the freezing and microwave thawing pretreatment is used for being combined with biological composite enzymatic hydrolysis and ultrasonic treatment technologies; the almond oligopeptides with high yield and high hydrolysis degree are prepared; the impurities are few; no bad odor exists; the product purity is high; and the efficacy is good.

The invention discloses a method for preparing konjac mannan oligosaccharide. The method comprises the following steps of: 1) uniformly mixing konjak and water, cutting and emulsifying; homogenizing under the pressure of 160 to 250 MPa and the pressure of 450 to 650 MPa in turn; and drying to obtain coarse konjak powder; and 2) performing enzyme hydrolysis reaction on the slurry of the coarse konjak powder and endo-mannase to obtain the generation liquid of the konjac mannan oligosaccharide, and continuously taking the generation liquid of the konjac mannan oligosaccharide out; replenishing the slurry of the coarse konjak powder once every 3 to 6 hours of the reaction; after replenishing for 9 to 20 times, reacting for 3 to 6 hours and stopping; and collecting and merging the generation liquid of the konjac mannan oligosaccharide, and drying to obtain the konjac mannan oligosaccharide. By the method, the effect and utilization rate of the mannase can be improved and the target productof the mannan oligosaccharide can be obtained to the maximum extent, and the purity of the product is over 90 percent.

The invention belongs to the technical field of feed quality detection, and particularly to a method for determining the selenium form in a selenium-rich feed, more particularly to a method for determining the selenium form in a selenium-rich feed by combining an ultrasonic probe-assisted enzymolysis technology and high-performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). According to the present invention, with the method, the content of the extracted selenium can be accurately determined, and the contents of the five forms of the selenium substances commonly contained in the selenium-rich feed can be effectively separated, such that the selenium form in the selenium-rich feed can be accurately analyzed, and the selenium-rich feed product can be rapidly and safely selected and used.

The invention discloses a method for determining selenium form in high-selenium fungi. The method comprises performing weak-base wall breaking processing and ball milling on a high-selenium sample, then performing enzymolysis by utilizing the processed sample and employing a step-by-step continuous extraction process, enabling enzymatic hydrolyzate obtained in enzymatic reaction at each step to pass a membrane, and applying to a machine of HPLC-HG-AFS and determining the selenium form. By employing the method for determining the selenium form in high-selenium fungi samples, the extraction rate is up to 50-80%, also the selenium form of the sample itself is stable, and variation of selenium form does not happen during reaction.

The invention discloses an efficient composite carbon source for denitrification treatment of low-carbon-nitrogen-ratio wastewater and a preparation method thereof, and belongs to the field of water treatment. The preparation is prepared from, by weight, 10-25% of glucose, 3-5% of molasses, 1-5% of sodium citrate, 10-20% of glycerol, 3-5% of sodium formate, 3-8% of sodium acetate, 1-3% of inorganic phosphorus salt, 0.5-1% of microelements, 0.5-2% of growth factors and the balance water; the composite biological carbon source preparation disclosed by the invention can meet the nutrition required by microorganisms in different growth stages in a microbiota, realizes efficient denitrification of wastewater, and can improve the growth rate of the microbiota and the utilization rate of a carbonsource matrix by adding inorganic phosphorus salt, trace elements, growth factors and the like according to the quality of the wastewater, thereby further improving the biological denitrification efficiency.

The invention discloses a method for preparing dietary fiber flour from sweet potato starch production waste. According to the method, a dietary fiber is prepared from sweet potato residues and liquid waste generated in the sweet potato starch production process; the sweet potato residues and nutrient substances contained in the liquid waste are also utilized to cultivate acetobacter xylinum as a carbon source and a partial nitrogen source, and are fermented to prepare bacterial cellulose; the raw materials are fully utilized; meanwhile, methods for carrying out ultra-low temperature frozen pretreatment on the sweet potato residues and carrying out high-pressure gas impact-assisted mixed enzymolysis are adopted in the preparation process; and the yield of the dietary fiber flour is increased and the purity of the dietary fiber flour is improved. According to the method, the sweet potato starch production waste can be fully utilized; the yield of the dietary fiber is increased, and the purity of the dietary fiber is improved; and the pollution to environment caused by sweet potato starch production is reduced.

The invention discloses a preparation method of V-shaped granular porous starch, belonging to the technical field of starch modification. The method comprises the following steps: firstly, treating starch by adopting a high-temperature alcohol method to prepare V-type starch; and then carrying out enzymolysis on the V-type starch in an ethanol system to prepare the V-type porous starch, wherein the temperature of the high-temperature alcohol method is 100-150 DEG C, and a mixed enzyme solution of alpha-amylase and saccharifying enzyme is adopted in the enzymolysis. The V-shaped porous starch is prepared by jointly using the high-temperature high-pressure alcohol method and an enzymolysis method to treat the starch; and the porous starch has a single-spiral V-shaped crystal structure and a higher specific surface area, so the water absorption and oil absorption capacities of the porous starch are obviously improved compared with porous starch prepared by a traditional method.

The invention discloses a preparing method for low-irritability infant nutritive rice flour. The preparing method includes the steps that organic rice serves as a main raw material, and smashing, screening, pulp mixing, stirring and ultrasonic treatment are conducted; a pH value of pulp is adjusted, trypsin and neutral protease are added, centrifugation is conducted, supernatant is filtered with an ultrafiltration membrane, a solution being smaller than 10 kDa and centrifugal solid are mixed and stirred, the pH value of the pulp is adjusted again, and mesophilic alpha-amylase is added for enzymolysis; the mixture is subjected to roller drying to obtain rice slices, cured grain powder, fruit powder, whole milk powder, soy isolate protein, concentrated whey protein, maltodextrin, fructo-oligosaccharide, galactooligosaccharide, probiotics, mineral substances and multivitamins are added, and are ground and mixed evenly, and sterilization and filling are conducted. The low-irritability infant nutritive rice flour prepared with the method is used for infants of 6 months or above to eat, the problems that an ingredient is single, functional ingredients are insufficient, polypeptide is bitter, the taste is poor, and digestion is difficult are solved, the immunity can be improved, sensitization is reduced, and the rice flour has wide application in the infant food field.

The invention relates to a method for promoting the decomposition of straw lignocellulose. The method comprises the following steps of: cutting up straws; placing in an oven at the temperature of between 55 and 65 DEG C for 5 to 7 hours; grinding by using a grinder until the particle size is 160 to 200 meshes; and grinding for 2 to 8 hours by using a nano ball mill. By the method, the structure of the straw lignocellulose can be relatively thoroughly broken, so that the particle sizes of the straws is more than the nano level, a subsequent enzymolysis reaction is facilitated, and the concentration of reducing sugar in the enzymolysis reaction is obviously improved and reaches 16mg.ml<-1>.

The formula of Laba bean food includes Laba bean, pungent and scenting agent, oil, seasonings and additive with the additive is one of chili, sugar, preserved ham and preserved fish. The Laba bean is produced with soybean and through cooking, cooling, fermentation with pure fermenting agent, canning and sterilizing. The Laba bean food may in different flavor for meeting the requirement of different people.

The invention provides a probiotics-enzyme fused fermentation culture capable of improving intestinal functions of livestock and poultry and a preparation method thereof, and belongs to the field of biological feed preparation. The probiotics-enzyme fused fermentation culture is characterized in that soybean meal, corn, bran, rice husk powder and molasses in different parts by mass are used as fermentation substrates, and the formula combination of compound probiotics and a compound enzyme preparation is optimized, and the culture is obtained by a probiotics-enzyme fused fermentation culture method. The preparation method is simple to operate, easy to implement and low in cost, and the prepared fermentation culture is rich in probiotics and metabolites, and has the functions of promoting animal ingestion, improving production performance of livestock and poultry, reducing intestinal diarrhea and other diseases.

The invention discloses a method for processing black tea. The method comprises the steps of picking, spreading for cooling, withering, rolling, fermenting, drying and packaging. Picked fresh tea leaves wither for 3-5 hours at 20-23 DEG C after being spread for cooling, the withered tea leaves are placed into a twisting machine to be twisted for 60-80 minutes and are fermented for 3-5 hours, the fermented tea leaves are dried for 20-30 minutes at 60-80 DEG C, and the tea leaves are dried for 10-20 minutes at 110-120 DEG C to enable the water content to reach 7% or below as to obtain finished raw tea. Shapeless tea leaves and impurities of the finished raw tea are removed, and the finished raw tea is packaged by bark paper or cotton cloth to be placed in a packing case or a packing box and stored at a cool and dry place. The black tea processing technology is simple and practical and large scale production can be achieved.

The invention provides a drawing protein for artificial meat and a preparation method of the drawing protein for the artificial meat. The preparation raw material of the drawing protein for the artificial meat comprises 4 to 5 parts of a vegetable protein, 4 to 5 parts of vital gluten, 1 to 2 parts of starch, 0.03 to 0.06 part of TG enzymes and 0.6 to 1 part of water in parts by weight. The drawing protein for the artificial meat provided by the invention has the advantages that through the specific proportion of the vegetable protein, the vital gluten, the starch, the TG enzymes and the water, all of the ingredients are compatible with each other; the produced drawing protein achieves a good shaping effect; and after rehydration, the protein is tough and elastic.

The invention relates to a deodorized garlicin extraction method. The method comprises the following steps: selecting high-quality garlic as a raw material, adding water for immersion, peeling the cuticle, flushing, crushing, adding to an enzymatic hydrolysis apparatus, adding water, adding an enzyme activator Fe<2+> enzyme, and carrying out enzymatic hydrolysis of alliinase in garlic under controlled conditions; carrying out steam heating distillation extraction, and cooling through a condenser to obtain an oil-water mixed solution; allowing the obtained oil-water mixed solution to stand for layering, and taking the obtained lower layer oil to obtain an oil-water distillate containing garlicin; adding salad oil for extraction deodorization; and allowing the obtained mixture to stand, and taking the bottom layer oil phase liquid to obtain a deodorized garlicin stock solution. The method has the advantages of reasonable technology, short extraction time, no organic solvent residual, good deodorization effect and high extraction efficiency; and the garlicin prepared through the method reserves all nutrition components in the raw material and has a good product safety.

The invention discloses a method for quickly separating and culturing fibroblast from human skin tissues. The method comprises the following steps of cleaning and shearing fresh foreskin tissues afteroperation of adults or children, and sequentially adopting solutions of dispase II and dispase I to digest under the condition of oscillation in a water bath at the temperature of 37 DEG C; and collecting skin tissue blocks through centrifuging; enabling a culture medium to resuspend, and culturing in a culture box with CO2 (carbon dioxide) volume concentration of 5% at the temperature of 37 DEGC. The method has the advantages that the temperature of the digestion function of the dispase I and the dispase II is increased from 4 DEG C to 37 DEG C, the activities of the dispase I and the dispase II are respectively improved, the contact areas between the dispase I as well as the dispase II and a corresponding primer are increased by the oscillation in the water bath, the enzymatic reaction is favorably performed, the digestion time of the dispase I as well as the dispase II is greatly shortened, and the separating and culture speed of the fibroblast is improved; the damage of enzyme to tissue cells is decreased, the wall attaching rate of the cells is improved, and the primary fibroblast can complete passage after culturing for 6 to 8 days.

The invention relates to a method for efficiently producing biodiesel from kitchen grease. The method comprises the following steps: (S1) pretreatment: carrying out hydrothermal treatment on kitchen waste, and carrying out three-phase separation on the treated kitchen waste; and (S2) preparing the biodiesel from grease: carrying out ester exchange reaction with short chain alcohol under the action of an enzyme catalyst so as to prepare rough biodiesel, and distilling an obtained product, so as to obtain refined biodiesel, wherein the step (S2) is carried out in an electric field. According to the method, biodiesel is prepared by virtue of an enzymic method, and electric field treatment is carried out during the preparation of the biodiesel, so that the yield of the biodiesel is remarkably increased.

The invention discloses a method for measuring the form of selenium in selenium-rich spiral seaweed. Wall breaking treatment is carried out on a selenium-rich spiral seaweed sample through lysozyme, then the treated sample is subjected to enzymolysis in a substep continuous extracting mode, and after enzymatic hydrolysate obtained through enzymatic reactions at all steps passes through a film, the form of selenium is measured through an HPLC-HG-AFS upper computer. The method is used for measuring the form of selenium in the selenium-rich spiral seaweed sample, the extraction ratio is high, the form of selenium of the sample is stable, and the form of selenium cannot change in the reaction process.

The invention discloses a plant hydrolat and a preparation method thereof. The preparation method comprises the following steps that S1, raw materials are treated; S2, steam distillation is carried out; S3, condensate is collected; S4, oil-water separation is carried out; S5, ultrafiltration of a crude hydrolat is carried out; S6, sterilization is caried out; S7, mixing is carried out; and S8, filling and delivery are carried out. The prepared plant hydrolat has high essential oil content, has a good moisturizing effect on skin after being used, and can achieve the effects of improving skin closed acne, shrinking pores and improving skin fineness.

The invention relates to a method for producing L-4-hydroxyisoleucine by a microbial enzyme conversion method. The method comprises the following steps: 1, culturing a mature bacterial strain; 2, grafting mature escherichia coli recombinant bacteria which are capable of inducing production of L-4-hydroxyisoleucine into a fermentation tank, and carrying out fermentation culture, wherein the temperature of the fermentation process is controlled in two stages, i.e., 4 to 36 DEG C in the earlier stage and 30 to 32 DEG C in the later stage; 3, collecting thallus after fermentation, adding phosphate buffer and washing, then adding alpha-oxoglutarate, isoleucine, ferrous sulfate, magnesium sulfate and ascorbic acid, and carrying out forced ventilation for catalysis; 4, removing bacteria from the catalytic liquid with a ceramic membrane, filtering with an ultrafiltration membrane, decoloring with activated carbon, desalting with faintly acid cation exchange resin, adsorbing effluent with 732 cation exchange resin and eluting with ammonia water, carrying out evaporation concentration and recrystallization to obtain the L-4-hydroxyisoleucine. The preparation method disclosed by the invention is simple and efficient in reaction system and simple and feasible in extraction process. The conversion rate is up to over 95.4 percent, and the purity of the product can reach over 98 percent.

The invention discloses a preparation method of mulberry leaf oolong tea. The preparation method comprises the following steps: picking mulberry leaves, cutting the mulberry leaves, carrying out ultrahigh pressure treatment, picking tea leaves, carrying out sun withering, carrying out enzyme treatment, carrying out green making, carrying out fixation and primarily drying. The preparation method has the advantages that by virtue of the ultrahigh pressure treatment, some cells are broken and the release of endogenous substances in the mulberry leaves is promoted, so that the preparation method is conducive to the reduction of the herbal flavor of the mulberry leaves and to the promotion of following enzymatic reaction. With the addition of a complex enzyme preparation, the decomposition of substances such as cellulose and protein into various soluble sugars and amino acids as well as the release of aroma substances can be promoted, the herbal smell of the mulberry leaves is remarkably eliminated, the aroma of the mulberry tea is enhanced and the taste of the tea is improved. By adding the tea leaves in the process of carrying out the green making on the mulberry leaves, the fermentation of the mulberry leaves is promoted by virtue of polyphenol substances and polyphenol oxidase in the tea leaves, so that the unique quality and aroma of the oolong tea are generated. The oolong tea prepared by the invention has the green appearance, is in the form of a screw, compact and uniform in texture, and has the fragrance of flowers when the tea is dried; and after being brewed, the oolong tea has the brightly yellow and green soup color, and tastes refreshing and mellow.

The invention discloses an external application medicinal salt bag which comprises a heating bag, a crude salt bag and a medicinal powder bag; 1000g of large granular crude salt is contained in the crude salt bag; 160g of traditional Chinese medicine powder is contained in the medicinal powder bag; after being mixed, the large granular crude salt and the traditional Chinese medicine powder are heated in the heating bag and then externally applied, wherein the traditional Chinese medicine powder comprises 30g of rhizoma zingiberis, 20g of radix clematidis, 15g of monkshood, 15g of ligusticum wallichii, 15g of prepared frankincense, 15g of prepared myrrh, 10g of radix angelicae pubescentis, 10g of asarum, 10g of camphor, 10g of carthamus tinctorius and 10g of garden balsam stem. According to the invention, thermal therapy and the transdermic absorption effect of medicines are effectively combined; the first-pass effect of liver and gastrointestinal inactivation are avoided; the constant blood concentration or pharmacological effect can be kept easily; the action time is prolonged; the medicine delivery frequency is reduced; the medicine application compliance of patients is increased; therefore, the treatment effect is increased; permeation of the effective components of medicines can be promoted by using a salt bag to hot compress; furthermore, local enzymatic reaction can be promoted; and thus, the medicinal components can take effect on affected area directly and effectively.

The invention relates to a preparation process of an emblic leafflower fruit enzyme product with mixed bacteria segmented fermentation and a prepared enzyme product, and belongs to the technical fieldof fruit and vegetable fermentation products. The preparation process comprises: (1) selection of raw materials; (2) superficial cutting treatment of fruit; 3) mold aerobic pre-fermentation; (4) anaerobic fermentation of lactic acid bacteria and yeast; (5) coarse filtration; (6) preparation of the enzyme product. Compared with the existing emblic leafflower fruit enzyme production technology, thepreparation process achieves the effect that the production equipment cost is reduced by more than 30% for the same amount of the enzyme produced, the enzyme yield is increased by more than 8%, and the fermentation efficiency is reduced from the existing 615 days and 309 days to 80-100 days, the emblic leafflower fruit enzyme production efficiency is greatly improved, and the quality and the yield of the emblic leafflower fruit enzyme are greatly improved.

The invention discloses a method for extracting collagen peptides from flounder scales. The method comprises pretreatment, extraction of collagen, extraction of the collagen peptides and purification,and specifically comprises the following steps: firstly, soaking the flounder scales in diluted hydrochloric acid to remove purities, performing extraction with an acetic acid solution, adding pepsase for enzymolysis to prepare the collagen, adding papain and anisyl acetone into the collagen solution for enzymolysis, and performing ultrafiltration to prepare the collagen peptides. The method hasthe beneficial effects that: a twice-enzymolysis method for preparing the collagen peptides from the flounder scales is low in cost, relatively high in enzymolysis efficiency and relatively short in reaction time, and the collagen peptides is uniform in molecular weight and relatively high in activity.

The present invention discloses a processing technology of dendrobium huoshanense flower tea. The processing method comprises the following steps: (1) color protecting: pickled dendrobium huoshanense flowers are washed using warm water and a color-protecting agent is used to conduct an atomizing treatment; (2) vacuum withering: the color-protected dendrobium huoshanense flowers are put into a vacuum tank to be subjected to a withering treatment respectively at a vacuum degree of 100% and 90%-95%; (3) magnetized oscillating: a magnetizer and ultrasonic wave are respectively used to treat the dendrobium huoshanense flowers; (4) stir-frying at a low temperature: firstly, mixed flowers are subjected to a low temperature stir-frying, the color-protecting agent spraying treated dendrobium huoshanense flowers are added, and the dendrobium huoshanense flowers are subjected to a slow stir-frying; and (5) freezing and drying: the stir-fried dendrobium huoshanense flowers are subjected to a freezing treatment, the frozen dendrobium huoshanense flowers are dried, and the dried dendrobium huoshanense flowers are sealedly packaged to prepare the dendrobium huoshanense flower tea. The prepared dendrobium huoshanense flower tea by the method has characteristics of being high in a polysaccharide content, high in a volatile oil content, long in shelf life, etc., and is natural in color and luster, and mellow and delicate in fragrance after being soaked.

The invention discloses an ultrasonic-assisted rubber seed oil extraction method which comprises the following steps: step one, preparation of rubber seed cake raw materials; step two, preparation of rubber seed crude oil; step three, acidizing: the rubber seed oil is subjected to acidizing, and acidized rubber seed oil is obtained; step four, first enzymolysis, first rubber seed oil is prepared; step five, second enzymolysis, second rubber seed oil is obtained; step six, third enzymolysis, third rubber seed oil is obtained; step seven, vacuum drying: the third rubber seed oil is subjected to vacuum drying treatment, and the finished product rubber seed oil is obtained. According to the ultrasonic-assisted rubber seed oil extraction method, the phospholipid content in the rubber seed oil ranges from 5.28 mg / Kg to 9.42 mg / Kg, the rubber seed oil is safe and environment-friendly, the preparation is convenient, and the oil quality is good.