Method for extracting collagen peptide from flounder scales

A technology of collagen peptides and collagen, which is applied in the field of extracting collagen peptides from flatfish scales, can solve the problems of environmental pollution and waste of resources, and achieve the effects of high activity, preventing catalysis, and reducing reaction time

Inactive Publication Date: 2018-06-19
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish scales are usually discarded as waste, which not only pollutes the environment, but also wastes resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for extracting collagen peptides from flounder scales, comprising the following steps:

[0014] 1) Clean the scales of flounder, soak in 0.5mol / L dilute hydrochloric acid solution for 45 minutes, wash with clean water to neutrality after treatment, then dry, pulverize, and pass through an 80-mesh sieve; 2) Accurately weigh the pretreatment The treated fish scale samples were added with 6 times the amount of 3% acetic acid solution, and then extracted at 2 °C for 12 hours; the acid extract was centrifuged at 2 °C and 8500 r / min for 20 minutes, and the supernatant was collected; The ratio of 1g:10mL is immersed in 3g / L pepsin solution, pH is adjusted to 1.2, temperature is 10℃, frequency is 40kHz, density is 0.50W / cm 2 3) Adjust the collagen solution to a concentration of 1.5%, adjust the pH to 6.2, then add papain with 4% by weight of collagen solution solute, and then add fennel with 5.0% by weight of papain Acetone, enzymatically hydrolyzed at 60 °C for 25 mi...

Embodiment 2

[0016] The method for extracting collagen peptides from flounder scales includes: pretreatment, extracting collagen, extracting collagen peptides, and purifying, and specifically includes the following steps:

[0017] 1) Pretreatment: Clean the scales of flounder, soak in 0.8mol / L dilute hydrochloric acid solution for 60 minutes, wash with clean water to neutrality after treatment, then dry, pulverize, and pass through a 100-mesh sieve; pretreatment can be removed The sludge, mucus, metal ions and other impurities in fish scales can reduce the impact of impurities on the extraction process and improve the extraction efficiency;

[0018] 2) Collagen extraction: Accurately weigh the pretreated fish scale samples, add 8 times the amount of 5% acetic acid solution, and then extract at 4°C for 24 hours; centrifuge the acid extract at 4°C and 9000 r / min For 40 minutes, the supernatant was collected; the remaining residue was immersed in a 5g / L pepsin solution at a ratio of 1g:15mL, ...

Embodiment 3

[0022] The method for extracting collagen peptides from flounder scales includes: pretreatment, extracting collagen, extracting collagen peptides, and purifying, and specifically includes the following steps:

[0023] Pretreatment: Clean the scales of flounder, soak them in 0.6mol / L dilute hydrochloric acid solution for 50 minutes, wash with clean water to neutrality after treatment, then dry, pulverize, and pass through an 80-mesh sieve; pretreatment can remove fish scales The sludge, mucus, metal ions and other impurities can reduce the influence of impurities on the extraction process and improve the extraction efficiency;

[0024]Collagen extraction: Accurately weigh the pretreated fish scale samples, add 7 times the amount of 4% acetic acid solution, and extract at 3°C ​​for 16 hours; centrifuge the acid extract for 30 minutes at 3°C ​​and 8600 r / min , collect the supernatant; the remaining residue is immersed in 4g / L pepsin solution at the ratio of 1g:12mL, adjust the pH...

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PUM

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Abstract

The invention discloses a method for extracting collagen peptides from flounder scales. The method comprises pretreatment, extraction of collagen, extraction of the collagen peptides and purification,and specifically comprises the following steps: firstly, soaking the flounder scales in diluted hydrochloric acid to remove purities, performing extraction with an acetic acid solution, adding pepsase for enzymolysis to prepare the collagen, adding papain and anisyl acetone into the collagen solution for enzymolysis, and performing ultrafiltration to prepare the collagen peptides. The method hasthe beneficial effects that: a twice-enzymolysis method for preparing the collagen peptides from the flounder scales is low in cost, relatively high in enzymolysis efficiency and relatively short in reaction time, and the collagen peptides is uniform in molecular weight and relatively high in activity.

Description

technical field [0001] The invention relates to the field of collagen peptide extraction, in particular to a method for extracting collagen peptide from flounder scales. technical background [0002] Flounder, also known as flounder, is a collective name for the bony fish class flounder. The body is very flat, oblong, oval or long tongue-shaped, and the maximum body length can reach 5 meters. The body of the adult fish is asymmetrical. Both eyes are located on the left or right side of the head. Mouth slightly protruding. Fins generally lack spines. The base of the dorsal and anal fins is long, connected or not connected to the caudal fin. Widely distributed in warm and hot waters of all oceans, mainly feeding on benthic invertebrates and fish. There are a wide variety of species, there are more than 540 species in the world, and 120 species are produced in China. Inhabits the sandy bottom of the shallow sea, feeding on small fish and shrimp. They are particularly su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/36
CPCC07K14/78C12P21/06
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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