Method for quickly separating and culturing fibroblast from human skin tissues
A fibroblast, separation and culture technology, applied in the field of rapid separation and culture of human skin tissue-derived fibroblasts, can solve the problems of reduced activity of human skin fibroblasts, low adherence rate of tissue blocks, low cell activity, etc. Digestion time, increased speed and survival, and the effect of eliminating cell aggregation
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[0030] In this embodiment, a method for rapidly separating and culturing fibroblasts derived from human skin tissue comprises the following steps:
[0031] Step 1. Take the fresh foreskin tissue discarded after surgery in adults or children, wash it with saline containing 100 U / mL penicillin and 100 U / mL streptomycin for 2 to 3 times in a biological safety cabinet, and then remove it with sterile scissors and tweezers. Adipose tissue on the underside of the foreskin and cut into 0.5cm 2 small pieces of foreskin;
[0032] Step 2. Put the small pieces of foreskin obtained in Step 1 into a dispase II solution with a mass concentration of 0.4%, and then place it at 37° C. and digest it for 1 hour under the condition of shaking in a water bath;
[0033] Step 3, adding twice the volume of normal saline to the dispase II solution digested in step 2 to terminate the digestion, then remove the epidermis of the prepuce with sterile scissors and tweezers to obtain the dermal tissue, and...
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