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Method for determining selenium form in high-selenium fungi

A high selenium and fungus technology, applied in the field of analysis and testing, can solve the problem of low extraction rate of selenium, and achieve the effects of improving extraction rate, ensuring stability and mild reaction conditions

Inactive Publication Date: 2016-01-27
SUZHOU INST FOR ADVANCED STUDY USTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is exactly to provide a kind of assay method of selenium form in high selenium fungus, aims to solve the problem that the extracting rate of selenium is not high in utilizing traditional survey form method process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the embodiment of selenium form determination of high-selenium Ganoderma lucidum sample

[0029] The total selenium content of the selected high-selenium ganoderma lucidum mycelia is 209.8 mg / kg (WW).

[0030] (1) Pretreatment of high-selenium Ganoderma lucidum samples: Wash high-selenium Ganoderma lucidum samples obtained from liquid deep selenium-enriched fermentation culture with 18.2MΩ ultrapure water to remove residual inorganic selenium on the surface;

[0031] (2) Sample wall breaking treatment: Weigh 10g of fresh Ganoderma lucidum, add 50mL of 0.01mol / L sodium hydroxide solution, and stir on a magnetic stirrer for 20min. Then centrifuge at a speed of 5000rpm for 15 minutes to remove the alkali solution, take the precipitated part and put it into a dialysis bag for dialysis treatment with ultrapure water to remove residual sodium hydroxide in the sample;

[0032] (3) Sample ball milling: Weigh 1 g of the above-mentioned sample that has been subject...

Embodiment 2

[0039] Embodiment 2: high-selenium Ganoderma lucidum sample selenium speciation embodiment

[0040] The total selenium content of the selected high-selenium ganoderma lucidum mycelia is 209.8 mg / kg (WW).

[0041] (1) Pretreatment of high-selenium Ganoderma lucidum samples: Wash high-selenium Ganoderma lucidum samples obtained from liquid submerged fermentation with 18.2MΩ ultrapure water to remove residual inorganic selenium on the surface;

[0042] (2) Sample wall breaking treatment: Weigh 10g of fresh Ganoderma lucidum, add 100ml of 0.05mol / L sodium hydroxide solution, place on a magnetic stirrer and stir for 20min. Then centrifuge at a speed of 5000rpm for 15 minutes to remove the alkali solution, take the precipitated part and put it into a dialysis bag for dialysis treatment with ultrapure water to remove residual sodium hydroxide in the sample;

[0043] (3) Sample ball milling: Weigh 2g of the above-mentioned sample that has been subjected to alkali wall breaking treatm...

Embodiment 3

[0050] Embodiment 3: the embodiment of selenium speciation determination of high selenium yeast sample

[0051] The total selenium content of the selected high-selenium yeast is 2000mg / kg (DW).

[0052] (1) Sample wall breaking treatment: Weigh 10g dry yeast powder, add 100ml 0.01mol / L sodium hydroxide solution, place on a magnetic stirrer and stir for 20min. Then centrifuge at a speed of 5000rpm for 15 minutes to remove the alkali solution, take the precipitated part and put it into a dialysis bag for dialysis treatment with ultrapure water to remove residual sodium hydroxide in the sample;

[0053] (2) Sample ball milling: Weigh 1g of the above-mentioned sample that has been subjected to alkali wall-breaking treatment, add 50ml of PBS buffer solution with a pH value of 7.5 for ball milling, the ball milling frequency is 30Hz, and the ball milling time is 4h;

[0054] (3) Sample enzymatic hydrolysis: Take 5ml of the above-mentioned ball-milled sample in a 15ml centrifuge tub...

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Abstract

The invention discloses a method for determining selenium form in high-selenium fungi. The method comprises performing weak-base wall breaking processing and ball milling on a high-selenium sample, then performing enzymolysis by utilizing the processed sample and employing a step-by-step continuous extraction process, enabling enzymatic hydrolyzate obtained in enzymatic reaction at each step to pass a membrane, and applying to a machine of HPLC-HG-AFS and determining the selenium form. By employing the method for determining the selenium form in high-selenium fungi samples, the extraction rate is up to 50-80%, also the selenium form of the sample itself is stable, and variation of selenium form does not happen during reaction.

Description

technical field [0001] The invention relates to a method for determining the form of selenium in high-selenium fungi, belonging to the field of analysis and testing. Background technique [0002] Selenium is an essential trace element for the human body and an essential component of glutathione peroxidase. Selenium has an antioxidant effect and is closely related to human health. Selenium has anti-cancer, heart protection, anti-hepatic necrosis, prevention and treatment of myopia and cataracts, It has multiple pharmacological effects such as detoxification, improving immunity, delaying aging and enhancing reproductive function. According to incomplete statistics, there are more than 40 countries and regions lacking selenium in the world, and 72% of the regions in my country are selenium-deficient areas. Scientists pay attention. [0003] Fungi are eukaryotic organisms. The most common fungi are all kinds of mushrooms. In addition, fungi also include molds and yeasts. The c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 尹雪斌袁林喜侯遇珠
Owner SUZHOU INST FOR ADVANCED STUDY USTC
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